The effect of freeze-thawing on the release of intracellular proteins from Escherichia coli by means of a bead mill

World Journal of Microbiology and Biotechnology

Volumn 13, Issue 4, 1997, Pages 475-477

  Bakir, U ^a   Hamamci, H ^a  

^a MIDDLE EAST TECHNICAL UNIVERSITY   (Turkey)

Author keywords

Bead milling; Disruption; E. coli; Freeze thawing

Indexed keywords

ESCHERICHIA COLI;

EID: 0030856595     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018592604304     Document Type: Article

References (7)

1. Agervist, I. & Enfors, S-O. 1990 Characterization of E. coli cell disintegrates from a bead mill and high pressure homogenizers. Biotechnology and Bioengineering 36, 1083-1089.
2. Bakir, U. Koçak, H.A. & Hamamci, H. 1988 The construction and use of bead mill for the extraction of intracellular proteins. Doga 12, 170-175.
3. Cole, M., Savidge, T. & Vanderhaeghe, H. 1975 Penicillin Acylase (Assay). Methods in Enzymology 43, 698-720.
4. Johnson, B. H. & Hecht, M.H. 1994 Recombinant proteins can be isolated from E. coli cells by repeated cycles of freezing and thawing. Bio/Technology 12, 1357-1360.
5. Paoletti, L. C., Short, A. & Blakemore, R.P. 1987 Freeze-thawing of Aquaspirillium magnetotacticum cells selectively releases periplasmic proteins. Applied and Environmental Microbiology 53, 2590-2592.
6. Van Gaver, D. & Huyghebaert, A. 1991 Optimization of yeast cell disruption with a newly designed bead mill. Enzyme and Microbiological Technology 13, 665-671.
7. Woodrow, J.R. & Quirk, A.V. 1982 Evaluation of the potential of a bead mill for the release of intracellular bacterial enzymes. Enzyme and Microbial Technology 4, 385-389.