Epitope and mimotope for an antibody to the Na,K-ATPase

Protein Science

Volumn 6, Issue 7, 1997, Pages 1537-1548

  Pacholczyk, Tadeusz ^a   Sweadner, Kathleen J ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Antibody mapping; Epitope determination; Membrane protein structure; Phage display of peptides; Random fragment library screening; Synthetic fusion proteins

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); BETA GALACTOSIDASE; CHIMERIC PROTEIN; COMPLEMENTARY DNA; EPITOPE; HYBRID PROTEIN; MONOCLONAL ANTIBODY; PROLINE;

AMINO TERMINAL SEQUENCE; ARTICLE; CONSENSUS SEQUENCE; ENZYME ANALYSIS; ENZYME DEGRADATION; NONHUMAN; PRIORITY JOURNAL;

EID: 0030854894     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560060718     Document Type: Article

References (38)

1. Amit AG, Mariuzza RA, Phillips SE, Poljak RJ. 1986. Three-dimensional structure of an antigen-antibody complex at 2.8Å resolution. Science 233:747-753.
2. Arystarkhova E, Sweadner KJ. 1996. Isoform-specific monoclonal antibodies to Na-K-ATPase α subunits: Evidence for a tissue-specific post-translational modification of the α subunit. J Biol Chem 271:23407-23417.
3. Balass M, Heldman Y, Cabilly S, Givol D, Katchalski-Katzir E, Fuchs S. 1993. Identification of a hexapeptide that mimics a conformation-dependent binding site of acetylcholine receptor by use of a phage-epitope library. Proc Natl Acad Sci USA 90:10638-10642.
4. Ball T, Vrtala S, Sperr WR, Valent P, Susani M, Kraft D, Valenta R. 1994. Isolation of an immunodominant IgE hapten from an epitope expression cDNA library. Dissection of the allergic effector reaction. J Biol Chem 269:28323-28328.
5. Bottger V, Bottger A, Lane EB, Spruce BA. 1995. Comprehensive epitope analysis of monoclonal anti-proenkephalin antibodies using phage display libraries and synthetic peptides: Revelation of antibody fine specificities caused by somatic mutations in the variable region genes. J Mol Biol 247:932-946.
6. Brines ML, Gulanski BI, Gilmore-Hebert M, Greene AL, Benz EJ Jr, Robbins RJ. 1991. Cytoarchitectural relationships between ouabain binding and mRNA for isoforms of the sodium pump catalytic subunit in rat brain. Brain Res Mol Brain Res 10:139-150.
7. Cwirla SE, Peters EA, Barrett RW, Dower WJ. 1990. Peptides on phage: A vast library of peptides for identifying ligands. Proc Natl Acad Sci USA 57:6378-6382.
8. Daly SE, Lane LK, Blostein R. 1996. Structure/function analysis of the amino-terminal region of the alpha1 and alpha2 subunits of Na,K-ATPase. J Biol Chem 39:23683-23689.
9. Devlin JJ, Panganiban LC, Devlin PE. 1990. Random peptide libraries: A source of specific protein binding molecules. Science 249:404-406.
10. Felsenfeld DP, Sweadner KJ. 1988. Fine specificity mapping and topography of an isozyme-specific epitope of the Na,K-ATPase catalytic subunit. J Biol Chem 263:10932-10942.
11. Hoess R, Brinkmann U, Handel T, Pastan I. 1993. Identification of a peptide which binds to the carbohydrate-specific monoclonal antibody B3. Gene 128:43-49.
12. +)ATPase. Biochemistry 25:569-573.
13. +-ATPase. Meth Enzymol, 156:291-301.
14. Kay BK, Adey NB, He YS, Manfredi JP, Mataragnon AH, Fowlkes DM. 1993. An M13 phage ligrary displaying random 38-amino acid peptides as a source of novel sequences with affinity to selected targets. Gene 128:59-65.
15. Landon M. 1977. Cleavage at aspartyl-prolyl bonds. Meth Enzymol 47:145-149.
16. Lischwe MA, Ochs D. 1982. A new method for partial peptide mapping using N-chlorosuccinimide/urea and peptide silver staining in sodium dodecyl sulfate-polyacrylamide gels. Anal Biochem 127:453-457.
17. Lutsenko S, Kaplan JH. 1995. Organization of P-type ATPases: Significance of structural diversity. Biochemistry 54:15607-15613.
18. +)-ATPase alpha and alpha(+) isozymes are the products of different genes. Biochem Biophys Res Commun 132:164-169.
19. +-ATPase using a random peptide library. Protein Sci 2:2103-2111.
20. Marchuk D, Drumm M, Saulino A, Collins FS. 1990. Construction of T-vectors, a rapid and general system for direct cloning of unmodified PCR products. Nucleic Acids Res 19:1154.
21. McGrail KM, Phillips JM, Sweadner KJ. 1991. Immunofluorescent localization of three Na,K-ATPase isozymes in the rat central nervous system: Both neurons and glia can express more than one Na,K-ATPase. J Neurosci 17:381-391.
22. McLafferty MA, Kent RB, Ladner RC, Markland W. 1993. M13 bacteriophage displaying disulfide-constrained microproteins. Gene 125:29-36.
23. Mehra V, Sweetser D, Young RA. 1986. Efficient mapping of protein antigenic determinants. Proc Natl Acad Sci USA 55:7013-7017.
24. Oldenburg KR, Loganathan D, Goldstein IJ, Schultz PG, Gallop MA. 1992. Peptide ligands for a sugar-binding protein isolated from a random peptide library. Proc Natl Acad Sci USA 59:5393-5397.
25. Roberts D, Guegler K, Winter J. 1993. Antibody as a surrogate receptor in the screening of a phage display library. Gene 128:61-69.
26. Scott JK, Smith GP. 1990. Searching for peptide ligands with an epitope library. Science 249:386-390.
27. +-ATPase alpha-subunit from rat brain. Biochemistry 25:8125-8132.
28. +)-ATPase. J Biol Chem 261:16788-16791.
29. Shull MM, Pugh DG, Lingrel JB. 1989. Characterization of the human Na,K-ATPase alpha 2 gene and identification of intragenic restriction fragment length polymorphisms. J Biol Chem 264:17532-17543.
30. Shyjan AW, Levenson R. 1989. Antisera specific for the alpha 1, alpha 2, alpha 3, and beta subunits of the Na,K-ATPase: differential expression of alpha and beta subunits in rat tissue membranes. Biochemistry 25:4531-4535.
31. Stephen CW, Helminen P, Lane DP. 1995. Characterisation of epitopes on human p53 using phage-displayed peptide libraries: Insights into antibody-peptide interactions. J Mol Biol 245:58-78.
32. +-ATPase from mammalian axolemma. Meth Enzymol 156:65-71.
33. +-ATPase. Biochim Biophys Acta 988:185-220.
34. Sweadner KJ, Gilkeson RC. 1985. Two isozymes of the Na,K-ATPase have distinct antigenic determinants. J Biol Chem 260:9016-9022 .
35. +-ATPase alpha-subunit isoforms in evolution. Am J Physiol 259:C619-C630 .
36. Urayama O, Shutt H, Sweadner KJ. 1989. Identification of three isozyme proteins of the catalytic subunit of the Na,K-ATPase in rat brain. J Biol Chem 264:8271-8280.
37. Urayama O, Sweadner KJ. 1988. Ouabain sensitivity of the alpha 3 isozyme of rat Na,K-ATPase. Biochem Biophys Res Commun 156:796-800.
38. +-ATPase alpha-and beta-subunit isoforms within the rat central nervous system. Proc Natl Acad Sci USA 88:7425-7429.