A simple and efficient PCR method for the specific detection of Pseudomonas syringae pv phaseolicola in bean seeds

World Journal of Microbiology and Biotechnology

Volumn 13, Issue 4, 1997, Pages 463-467

  Mosqueda Cano, G ^a   Herrera Estrella, L ^a  

^a Laboratorio Nacional de Genómica para la Biodiversidad   (Mexico)

Author keywords

Halo blight; Pathogen detection; PCR; Phaseolus vulgaris; Pseudomonas syringae

Indexed keywords

BACTERIA (MICROORGANISMS); PHASEOLUS (ANGIOSPERM); PHASEOLUS VULGARIS; PSEUDOMONAS; PSEUDOMONAS SYRINGAE; PSEUDOMONAS SYRINGAE PV. PHASEOLICOLA;

EID: 0030854085     PISSN: 09593993     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018588503396     Document Type: Article

References (17)

1. Bej, A.K., Dicesare, J.L., Heff, L. & Atlas, R.M. 1991 Detection of E. coli and Shigella spp. in water using the polymerase chain reaction and gene probes for uid. Applied and Environmental Microbiology 57, 1013-1017.
2. Chowdhury, K. 1991 One step 'miniprep' method for the isolation of plasmid DNA. Nucleic Acids Research 19, 2792-2796.
3. Deblaere, R., Bytebier, B., de Greve, H., Deboeck, F., Schell, J., van Montagu, M. & Leemans, J. 1985 Efficient octopine Ti plasmid-derived vectors for Agrobacterium-mediated gene transfer to plants. Nucleic Acids Research 13, 4777-1788.
4. Guthrie, J.W., Huber, D.M. & Fenwick, H.S. 1965 Serological detection of halo-blight. Plant Disease Reports 49, 297-299.
5. Herrera-Estrella, L. & Simpson, J. 1988 In Foreign gene expression in plants, ed, Shaw, C.H. pp. 131-160. Oxford: IRL Press.
6. Lahman, L.K. & Schaad, N.W. 1985 Evaluation of the "Dome Test" as a reliable assay for seedborne bacterial blight pathogens of beans. Plant Disease. 69, 680-683
7. Lelliot, R.A. & Stead, D.E. 1987 Methods for the diagnosis of bacterial diseases of plants. In Methods in plant pathology, Vol 2, ed Preece T.F. pp. 176-177. Oxford: Blackwell Scientific Publications.
8. Maniatis, T., Fritz, E.F. & Sambrook J. 1982 Molecular Cloning. A Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
9. Mohan, S.K. & Schaad, N.W. 1987 An improved agar plating assay for detecting Pseudomonas syringae pv. syringae and P. s. pv. phaseolicola in contamined bean seed. Phytopathology 77, 1390-1395.
10. Mosqueda-Cano, G., van den Broeck, G., Saucedo, O., Bailey, A.M., Alvarez-Morales, A. & Herrera-Estrella, L. 1990 Isolation and characterization of the gene from Pseudomonas syringae pv. phaseolicola encoding the phaseolotoxin-insensitive ornithine carbamoyltransferase. Molecular and General Genetics 222, 461-466.
11. Patil, S.S., Kolattukudy, P.E. & Dimond, A.E. 1970 Inhibition of ornithine carbamoyltransferase from bean plants by the toxin of Pseudomonas phaseolicola. Plant Physiology 46, 752-753.
12. Prosen, D., Hatziloukas, E., Schaad, N.W. & Panopoulos, N.J. 1993 Specific detection of Pseudomonas syringae pv. phaseolicola DNA in bean seed by polymerase chain reaction based amplification of a phaseolotoxin gene region. Phytopathology 83, 965-970.
13. Schaad, N.W., Cheong, S.S., Tamaki, E., Hatziloukas, E. & Panopulos, N. J. A. 1995 Combined biological and enzymatic amplification (BIO-PCR) technique to detect Pseudomonas syringae pv. phaseolicola in bean seed extracts. Phytopathology 85, 243-248.
14. Schwartz, H.F & Pastor-Corrales, M.A. (eds). 1989 Bean production problems in the tropics. 2nd edn. Colombia: Cali.
15. Staskawicz, B.J., Panopoulos, N.J & Hoogenraad, N.J. 1980 Phaseolotoxin-insensitive ornithine carbamoyltransferase of Pseudomonas syringae pv. phaseolicola: Basis for immunity to phaseolotoxin. Journal of Bacteriology 142, 720-723.
16. Steffan, R.L. & Atlas, R.M. 1991 Polymerase chain reaction: Applications in environmental microbiology. Annual Review of Microbiology 45, 137-161.
17. Van Vuurde, J.W.L., van den Bovenkamp, G.A & Birnbaum, Y. 1983 Immunofluorescence microscopy and enzyme-linked immunosorbent assay as potential routine tests for the detection of Pseudomonas syringae pv. phaseolicola and Xanthomonas campestris pv. phaseoli in bean seed. Seed Science and Technology 11, 547-559.