Early colonization of barley roots by Pseudomonas fluorescens studied by immunofluorescence technique and confocal laser scanning microscopy

FEMS Microbiology Ecology

Volumn 23, Issue 4, 1997, Pages 353-360

  Hansen, Michael ^a   Kragelund, Lene ^a   Nybroe, Ole ^a   Sørensen, Jan ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Confocal laser scanning microscopy; Immunofluorescence; Pseudomonas fluorescens; Root colonization

Indexed keywords

BARLEY; IMMUNOFLUORESCENCE; LASER SCANNING MICROSCOPY; RHIZOPLANE; ROOT COLONIZATION;

ARTICLE; BACTERIAL COLONIZATION; CONFOCAL LASER MICROSCOPY; IMMUNOFLUORESCENCE TEST; NONHUMAN; PLANT ROOT; PRIORITY JOURNAL; PSEUDOMONAS FLUORESCENS;

HORDEUM VULGARE; POACEAE; PSEUDOMONAS FLUORESCENS;

EID: 0030854009     PISSN: 01686496     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-6496(97)00037-8     Document Type: Article

References (23)

1. Bennett, R.A. and Lynch, J.M. (1981) Bacterial growth and development in the rhizosphere of gnotobiotic cereal plants. J. Gen. Microbiol. 125, 95-102.
2. Rovira, A.D., Newman, E.I., Bowen, H.J. and Campbell, R. (1974) Quantitative assessment of the rhizoplane microflora by direct microscopy. Soil Biol. Biochem. 6, 211-216.
3. Newell, S.Y., Fallon, R.D. and Tabor, P.S. (1986) Direct microscopy of natural assemblages. In: Bacteria in Nature, Vol. 2, Methods and Special Applications in Bacterial Ecology (Poindexter, J.S. and Leadbetter, E.R., Eds.), pp. 1-48. Plenum Press, New York.
4. Foster, R.C. and Rovira, A.D. (1973) The rhizosphere of wheat roots studied by electron microscopy of ultra-thin sections. Bull. Ecol. Res. Commun. Stockholm 17, 93-102.
5. Foster, R.C. and Rovira, A.D. (1976) Ultrastructure of wheat rhizosphere. New Phytol. 76, 343-352.
6. Campbell, R. and Porter, R. (1982) Low temperature scanning electron microscopy of microorganisms in soil. Soil Biol. Biochem. 14, 241-145.
7. Costerton, J.W., Nickel, J.C. and Ladd, T.I. (1986) Direct microscopy of natural assemblages. In: Bacteria in Nature, Vol. 2, Methods and Special Applications in Bacterial Ecology. (Poindexter, J.S. and Leadbetter, E.R., Eds.), pp. 49-84. Plenum Press, New York.
8. Heijnen, C.E., Page, S. and van Elsas, J.D. (1995) Metabolic activity of Flavobacterium strain P25 during starvation and after introduction into bulk soil and the rhizosphere of wheat. FEMS Microbiol. Ecol. 18, 129-138.
9. Kragelund, L. and Nybroe, O. (1996) Competition between Pseudomonas fluorescens Ag1 and Alcaligenes eutrophus JMP134 (pJP4) during colonization of barley roots. FEMS Microbiol. Ecol. 20, 41-51.
10. Shank, S.C., Smith, R.L., Weiser, G.C., Zuberer, D.A., Bouton, J.H., Quesenberry, K.H., Tyler, M.E., Milam, J.R. and Littell, R.C. (1979) Fluorescent antibody technique to identify Azospirillum brasilense associated with roots of grass. Soil Biol. Biochem. 11, 287-295.
11. Assmus, R., Hutzler, P., Kirchhof, G., Amann, R., Lawrence, J.R. and Hartmann, A. (1995) In situ localization of Azospirillum brasilense in the rhizosphere of wheat with fluorescently labelled, rRNA-targeted oligonucleotide probes and scanning confocal laser microscopy. Appl. Environ. Microbiol. 61, 1013-1019.
12. Osborn, R.M., Schroth, M.N., Hancock, J.G. and Hendson, M. (1989) Dynamics of sugar beet seed colonization by Pythium ultimum and Pseudomonas species: Effects of seed rot and damping off. Phytopathology 79, 709-716.
13. Loper, J.E. and Schroth, M.N. (1986) Influence of bacterial sources of indole-3-acetic acid on root elongation of sugar beet. Phytopathology 76, 386-389.
14. Sørensen, J., Skouv, J., Jørgensen, A. and Nybroe, O. (1992) Rapid identification of environmental isolates of Pseudomonas aeruginosa, P. fluorescens and P. putida by SDS-PAGE analysis of whole-cell protein patterns. FEMS Microbiol. Ecol. 101, 41-50.
15. Wilson, M. and Lindow, S.E. (1994) Coexistence among epiphytic populations mediated through nutritional ressource partitioning. Appl. Environ. Microbiol. 60, 4468-4477.
16. Harboe, N.M.G. and Ingild, A. (1983) Immunization, isolation of imunoglobulins and antibody titre determination. Scand. J. Immunol. 17, 345-351.
17. Kragelund, L., Leopold, K. and Nybroe, O. (1996) Outer membrane protein heterogeneity within Pseudomonas fluorescens and P. putida and use of an OprF antibody as a probe for rRNA homology group I pseudomonads. Appl. Environ. Microbiol. 62, 480-485.
18. Nybroe, O., Johansen, A. and Laake, M. (1990) Enzyme-linked immunosorbent assays for detection of Pseudomonas fluorescens in sediment samples. Lett. Appl. Microbiol. 11, 293-296.
19. Johnson, G.D. and de Noguira Araujo, G.M. (1981) A simple method of reducing the fading of immunofluorescence during microscopy. J. Immunol. Methods 43, 349-350.
20. Schloter, M., Borlinghaus, R., Bode, W. and Hartmann, A. (1993) Direct identification, and localization of Azospirillum in the rhizosphere of wheat using fluorescence-labelled monoclonal antibodies and confocal scanning laser microscopy. J. Microsc. 171, 173-177.
21. Scher, F.M., Kloepper, J.W., Singleton, C., Zaleska, I. and Laliberte, M. (1988) Colonization of soybean roots by Pseudomonas and Serratia species: Relationship to bacterial motility, chemotaxis and generation time. Phytopathology 78, 1055-1059.
22. Misaghi, I.J., Olsen, M.W., Bilotte, J.M. and Sonoda, R.M. (1992) The importance of rhizobacterial mobility in biocontrol of bacterial wilt of tomato. Soil Biol. Biochem. 24, 287-293.
23. Vande Broek, A. and Vanderleyden, J. (1996) The role of bacterial motility, chemotaxis and attachment in bacteria-plant interactions. Mol. Plant-Microbe Interact. 6, 800-810.