Expression and chloroplast-targeting of active phosphoenolpyruvate synthetase from Escherichia coli in Solanum tuberosum

Plant Science

Volumn 127, Issue 2, 1997, Pages 191-205

  Panstruga, Ralph ^a   Hippe Sanwald, Sigrun ^b   Lee, Yeon Kyeong ^c   Lataster, Marco ^a   Lipka, Volker ^a   Fischer, Rainer ^a   Liao, Yu Cai ^a   Häusler, Rainer E ^d   Kreuzaler, Fritz ^a   Hirsch, Heinz Josef ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

^b UNIVERSITY OF KIEL   (Germany)

^c Korea University   (South Korea)

^d UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Chloroplast transit sequence; Immunogold labeling; LT embedding; P(i) dikinase; Phosphoenolpyruvate synthetase; Pyruvate; Transgenic potato

Indexed keywords

PHOSPHOENOLPYRUVATE; SYNTHETASE;

ARTICLE; CHIMERA; CHLOROPLAST; ENZYME INDUCTION; ENZYME SUBUNIT; ESCHERICHIA COLI; IMMUNOBLOTTING; NONHUMAN; PROMOTER REGION; PROTEIN TARGETING; TRANSGENIC PLANT;

ESCHERICHIA COLI; SOLANUM TUBEROSUM;

EID: 0030852922     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-9452(97)00134-9     Document Type: Article

References (37)

1. G.E. Edwards and M.S.B. Ku, Biochemistry of C3-C4 intermediates, in: M.D. Hatch, N.K. Boardman (Eds.), Photosynthesis. The Biochemistry of Plants, vol. 10, Academic Press, New York, 1987, pp. 275-325.
2. A. Shomer-Ilan, S. Beer, Y. Waisel, Biochemical basis of ecological adaptation, in: M. Gibbs, E. Latzko (Eds.), Encyclopedia of Plant Physiology, vol. 6, Photosynthesis II. Photosynthetic Carbon Metabolism and Related Processes, Springer, Berlin, 1979, pp. 190-201.
3. A.R. Reddy, V.S.R. Das, Pyruvate phosphate dikinase-modulated fructose-2,6-bisphosphate content in Sorghum leaves in relation to varying light intensity, Biochem. Intern. 20 (1990) 25-35.
4. K. Aoyagi, J.A. Bassham, Pyruvate orthophosphate dikinase of C3 seeds and leaves as compared to the enzyme from maize, Plant Physiol. 75 (1984) 387-392.
5. M. Niersbach, F. Kreuzaler, R.H. Geerse, P.W. Postma, H.J. Hirsch, Cloning and nucleotide sequence of the Escherichia coli K-12 ppsA gene, encoding PEP synthase, Mol. Gen. Genet. 231 (1992) 332-336.
6. C. Koncz, J. Schell, The promoter of TL-DNA gene 5 controls the tissue specific expression of chimeric genes carried by a novel type of A grobacterium binary vector, Mol. Gen. Genet. 204 (1986) 383-396.
7. D. Hanahan, Studies on transformation of Escherichia coli with plasmids, J. Mol. Biol. 166 (1983) 557-580.
8. A.H. Goldie, B.D. Sanwal, Genetic and physiological characterization of Escherichia coli mutants deficient in phosphoenolpyruvate carboxykinase activity, J. Bacteriol. 141 (1980) 1115-1121.
9. J.M. Short, J.M. Fernandez, J.A. Sorge, W.D. Huse, Lambda ZAP: A bacteriophage Lambda expression vector with in vivo excision properties, Nucleic Acids Res. 16 (1988) 7583-7600.
10. R. Töpfer, V. Matzeit, B. Gronenborn, J. Schell, H.H. Steinbiss, A set of plant expression vectors for transcriptional and translational fusions, Nucleic Acids Res. 15 (1987) 5890.
11. I. Becker, Konstruktion chimärer Phosphoenolpyruvat-Carboxylase Gene und ihre Expression in Solanum tuberosum, Dissertation, RWTH Aachen, 1990.
12. C. Fritz, F.P. Wolter, V. Schenkemeyer, T. Herget, P.H. Schreier, The gene family encoding the ribulose-(1,5)-bisphosphate carboxylase/oxgenase (Rubisco) small subunit of potato, Gene 137 (1993) 271-274.
13. M.D. Valdés-Hevia, R. de la Guerra, C. Gancedo, Isolation and characterization of the gene encoding phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae, FEBS Lett. 258 (1989) 313-316.
14. R. Höfgen, L. Willmitzer, Storage of competent cells for Agrobacterium tranformation, Nucleic Acids Res. 16 (1988) 9877.
15. S. Knapp, G. Coupland, H. Uhrig, P. Starlinger, F. Salamini, Transposition of the maize transposable element Ac in Solanum tuberosum, Mol. Gen. Genet. 213 (1988) 285-290.
16. 2-Stoffwechsels, Dissertation. RWTH Aachen, 1995.
17. C. Gebhardt, E. Ritter, T. Debener, U. Schachtschnabel, B. Walkemeier, H. Uhrig, F. Salamini, RFLP analysis and linkage mapping in Solanum tuberosum, Theor. Appl. Genet. 78 (1989) 65-75.
18. C. Puissant, L.M. Houdebine, An improvement of the single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction, BioTechniques 8 (1990) 148-149.
19. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular cloning: A laboratory manual, 2nd ed., Cold Spring Harbor Press, New York, 1989.
20. S.G. Barlett, A.R. Grossman, N.-H. Chua, In vitro synthesis and uptake of cytoplasmically-synthesized chloroplast proteins, in: M. Edelman, R.B. Hallick, N.-H. Chua (Eds.), Methods in Chloroplast Molecular Biology, Elsevier, Amsterdam, 1982 pp. 1081-1091.
21. R.A. Cooper, H.L. Kornberg, Phosphoenolpyruvate synthetase, in: J.M. Lowenstein (Ed.), Methods of Enzymology, vol XIII, Academic Press, New York, 1969, pp. 309-314.
22. H. Teraoka, K. Izui, H. Katsuki, Phosphoenolpyruvate carboxylase of Escherichia coli: multiple conformational states elicited by allosteric effectors, Biochemistry 13 (1974) 5121-5128.
23. R.C. Leegood, Enzymes of the Calvin cycle, in: P.J. Lea (Ed.), Methods in Plant Biochemistry, vol. 3, Academic Press, New York, 1990, pp. 15-37.
24. D.J. Arnon, Copper enzymes in chloroplasts. Polyphenol oxidase in β-vulgaris, Plant Physiol. 24 (1949) 1-15.
25. 700, the functional plastoquinone pool, and the ratio of the photosystems in spinach chloroplasts, J. Biol. Chem. 259 (1984) 14003-14010.
26. K.M. Berman, M. Cohn, Phosphoenolpyruvate synthetase of Escherichia coli, J. Biol. Chem. 245 (1970) 5309-5318.
27. S. Hippe-Sanwald, K.H. Marticke, M.J. Kieliszewski, S.C. Somerville, Immunogold localization of THRGP-like epitopes in the haustorial interface of obligate, biotrophic fungi on monocots, Protoplasma 178 (1994) 138-155.
28. S. Hippe, K. Döring, F. Kreuzaler, In situ localization of a foreign protein in transgenic plants by immunoelectron microscopy following high pressure freezing, freeze substitution and low temperature embedding, Eur. J. Cell Biol. 50 (1989) 230-234.
29. A.R. Kermode, Mechanisms of intracellular protein transport and targeting in the plant cells, Crit. Rev. Plant Sci. 15 (1996) 285-423.
30. K. Cline, M. Werner-Washburne, J. Andrews, K. Keegstra, Thermolysin is a suitable protease for probing the surface of intact pea chloroplasts, Plant Physiol. 75 (1984) 675-678.
31. M.A. Hayat, Colloidal gold, vol 1 and 2. Academic Press, New York, 1989.
32. G. von Heijne, J. Steppuhn, R.G. Herrmann, Domain structure of mitochondrial and chloroplast targeting peptides, Eur. J. Biochem. 180 (1989) 535-545.
33. Y. Gavel, G. von Heinje, A conserved cleavage-site motif in chloroplast transit peptides, FEBS Lett. 261 (1990) 455-458.
34. G.E. Edwards, S.C. Huber, C4 metabolism in isolated cells and protoplasts, in: M. Gibbs, E. Latzko (Eds.), Encyclopedia of Plant Physiology, vol. 6, Photosynthesis II. Photosynthetic carbon metabolism and related processes, Springer, Berlin, 1979, pp. 102-112.
35. D.M. Stark, K.P. Timmerman, G.F. Barry, J. Preiss, G. Kishore, Regulation of the amount of starch in plant tissues by ADP-glucose pyrophosphorylase, Science 258 (1992) 287-292.
36. K. Aoyagi, J.A. Bassham, Pyruvate orthophosphate dikinase in wheat leaves, Plant Physiol. 73 (1983) 853-854.
37. M.D. Valdés-Hevia, R. de la Guerra, C. Gancedo, Isolation and characterization of the gene encoding phosphoenolpyruvate carboxykinase from Saccharomyces cerevisiae, FEBS Lett. 258 (1989) 313-316.