Evaluation of fracture planes and cell morphology in complementary fractures of cultured cells in the frozen-hydrated state by field-emission secondary electron microscopy: Feasibility for ion localization and fluorescence imaging studies

Journal of Microscopy

Volumn 186, Issue 3, 1997, Pages 232-245

  Chandra, S ^a   Morrison, G H ^a  

^a Department of Molecular Biology and Genetics   (United States)

Author keywords

Atomic force microscopy; Calcium; Electron probe elemental analysis; Field emission secondary electron microscopy; Fluorescence imaging; Frozen hydrated cells; Ion microscopy; Laser scanning confocal microscopy; Near field scanning optical microscopy; SIMS

Indexed keywords

CALCIUM; CELL MEMBRANES; CONFOCAL MICROSCOPY; CYTOLOGY; ELECTRONS; FIELD EMISSION MICROSCOPES; FLUORESCENCE IMAGING; FRACTURE; HYDRATION; IONS; SCANNING ELECTRON MICROSCOPY; SECONDARY EMISSION; SURFACE ANALYSIS;

ATOMIC-FORCE-MICROSCOPY; ELECTRON PROBE; ELECTRON PROBE ELEMENTAL ANALYSE; FIELD- EMISSION SECONDARY ELECTRON MICROSCOPY; FLUORESCENCE IMAGING; FROZEN-HYDRATED CELL; ION MICROSCOPY; LASER SCANNING CONFOCAL MICROSCOPY; NEARFIELD SCANNING OPTICAL MICROSCOPY; SECONDARY ELECTRON MICROSCOPY;

ATOMIC FORCE MICROSCOPY;

ANIMAL CELL; ARTICLE; ATOMIC FORCE MICROSCOPY; CELL ULTRASTRUCTURE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ELECTRON MICROSCOPY; ELECTRON PROBE MICROANALYSIS; FREEZE FRACTURE; IMAGE QUALITY; KIDNEY CELL; NONHUMAN; PRIORITY JOURNAL; SCANNING NEAR FIELD OPTICAL MICROSCOPY; SWINE;

ANIMALIA; SUS SCROFA;

EID: 0030852567     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1997.2030763.x     Document Type: Article

References (16)

1. Chandra, S., Fewtrell, C., Millard, P.J. Sandison, D.R., Webb, W.W. & Morrison, G.H. (1994) Imaging of total intracellular calcium and calcium influx and efflux in individual resting and stimulated tumor mast cells using ion microscopy. J. Biol. Chem. 269, 15186-15194.
2. Chandra, S., Kable, E.P.W., Morrison, G.H. & Webb, W.W. (1991) Calcium sequestration in the Golgi apparatus of cultured mammalian cells revealed by laser scanning confocal microscopy and ion microscopy. J. Cell Sci. 100, 747-752.
3. Chandra, S. & Morrison, G.H. (1985) Imaging elemental distribution and ion transport in cultured cells with ion microscopy. Science, 228, 1545-1544.
4. Chandra, S. & Morrison, G.H. (1995) Imaging ion and molecular transport at subcellular resolution by secondary ion mass spectrometry. Int. J. Mass Spectrom. Ion Proc. 143, 161-176.
5. Chandra, S., Morrison, G.H. & Wolcott, C.C. (1986) Imaging intracellular elemental distribution and ion fluxes in cultured cells with ion microscopy: A freeze-fracture methodology. J. Microsc. 144, 15-37.
6. Goldberg, M.W. & Allen, T.D. (1992) High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores. J. Cell Biol. 119, 1429-1440.
7. Johnson, L.V., Walsh, M.L. & Chen, L.B. (1980) Localization of mitochondria in living cells with rhodamine 123. Proc. Natl. Acad. Sci. U.S.A. 77, 990-994.
8. + pump sites in cultured pig kidney cells. Am. J. Physiol. 236 (3), C157-C162.
9. Nagatani, T., Saito, S., Sato, M. & Yamada, M. (1987) Development of an ultra high-resolution scanning electron microscope by means of a field emission source and in-lens system. Scanning Microsc. 1, 901-909.
10. Pscheid, P., Schudt, C. & Plattner, H. (1981) Cryofixation of monolayer cell cultures for freeze-fracturing without chemical pre-treatments. J. Microsc. 121, 149-167.
11. Ris, H. & Malecki, M. (1993) High-resolution field emission scanning electron microscope imaging of internal cell structures after epon extraction from sections: a new approach to correlative ultrastructural and immunocytochemical studies. J. Struct. Biol. 111, 148-157.
12. Somlyo, A.P., Somlyo, A.V. & Shuman, H. (1979) Electron probe analysis of vascular smooth muscle: composition of mitochondria, nuclei. and cytoplasm. J. Cell. Biol. 81, 316-335.
13. Wells, K.S., Sandison, D.R., Strickler J. & Webb, W.W. (1990) Quantitative fluorescence imaging with laser scanning confocal microscopy. Handbook of Biological Microscopy (ed. by J. B. Pawley), pp. 27-39. Plenum, New York.
14. 11SH, at subcellular resolution in tissue cultures using ion microscopy. Cancer Res. 52, 5219-5222.
15. 1 cells. Am. J. Physiol. 268 (Cell Physiol. 37), C1133-C1140.
16. Zierold, K. (1981) X-ray microanalysis of tissue culture cells in SEM and STEM. Scanning Electron Microsc. II, 409-418.