Modification of an in situ renaturation method for analysis of protein kinase activity with multiple substrates

BioTechniques

Volumn 23, Issue 4, 1997, Pages 652-657

  Fox, Theodore C ^a   Rumpho, Mary E ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN KINASE;

ANAEROBIC GROWTH; ANALYTIC METHOD; ARTICLE; ENZYME ACTIVITY; ENZYME SUBSTRATE; ENZYME SUBUNIT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN DENATURATION; PROTEIN MODIFICATION; TECHNIQUE;

EID: 0030851806     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97234bm24     Document Type: Article

References (6)

1. Cooper, J.A., B.M. Sefton and T. Hunter. 1983. Detection and quantification of phosphotyrosine in proteins. Methods Enzymol. 99:387-405.
2. Fallon, K.M. and A.J. Trewavas. 1994. Phosphorylation of a renatured protein from etiolated wheat leaf protoplasts is modulated by blue and red light. Plant Physiol. 105:253-258.
3. Geahlen, R.L., M. Anostario, Jr., P.S. Low and M.L. Harrison. 1986. Detection of protein kinase activity in sodium dodecyl sulfate-polyacrylamide gels. Anal. Biochem. 153: 151-158.
4. Lacks, S.A. and S.S. Springhorn. 1980. Renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. J. Biol. Chem. 255:7476-7484.
5. Moisyadi, S., S. Dharmasiri, H.M. Harrington and T.J. Lukas. 1994. Characterization of a low molecular mass autophosphorylating protein in cultured sugarcane cells and its identification as a nucleoside diphosphate kinase. Plant Physiol. 104:1401-1409.
6. Mujer, C.V., T.C. Fox, A.S. Williams, D.L. Andrews, R.A. Kennedy and M.E. Rumpho. 1995. Purification, properties and phosphorylation of anaerobically induced enolase in Echinochloa phyllopogon and E. cruspavonis. Plant Cell Physiol. 36:1459-1470.