Rapid kinetics of insertion and accessibility of spin-labeled phospholipid analogs in lipid membranes: A stopped-flow electron paramagnetic resonance approach

Biophysical Journal

Volumn 73, Issue 3, 1997, Pages 1645-1654

  Marx, Uwe ^a   Lassmann, Günter ^b   Wimalasena, Kandatege ^c   Müller, Peter ^a   Herrmann, Andreas ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

^b TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

^c WICHITA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CELL MEMBRANE; CHEMICAL ANALYSIS; ELECTRON SPIN RESONANCE; LIPID ANALYSIS; LIPID MEMBRANE; PHOSPHOLIPID BILAYER;

BOVINAE;

EID: 0030851621     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(97)78196-7     Document Type: Article

References (31)

1. Auland, M. E., B. D. Roufogalis, P. F. Devaux, and A. Zachowski. 1994. Reconstitution of ATP-dependent aminophospholipid translocation in proteoliposomes. Proc. Natl. Acad. Sci. USA. 91:10938-10942.
2. Bitbol, M., and P. F. Devaux. 1988. Measurement of outward translocation of phospholipids across human erythrocyte membrane. Proc. Natl. Acad. Sci. USA. 85:6783-6787.
3. Buton, X., G. Morrot, P. Fellmann, and M. Seigneuret. 1996. Ultrafast glycero-phospholipid-selective transbilayer motion mediated by a protein in the endoplasmic reticulum membrane. J. Biol. Chem. 271: 6651-6657.
4. Calvez, J.-Y., A. Zachowski, A. Herrmann, G. Morrot, and P. F. Devaux. 1988. Asymmetric distribution of phospholipids in spectrin-poor erythrocyte vesicles. Biochemistry. 27:5666-5670.
5. Connor, J., K. Gillum, and A. J. Schroit. 1990. Maintenance of lipid asymmetry in red blood cells and ghosts: effect of divalent cations and serum albumin on the transbilayer distribution of phosphatidylserine. Biochim. Biophys. Acta. 1025:82-86.
6. Devaux, P. F. 1991. Static and dynamic asymmetry in cell membranes. Biochemistry. 30:1163-1173
7. Dodge, J. T., C. Mitchell, and D. J. Hanahan. 1963. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch. Biochem. Biophys. 100:119-130.
8. Dolis, D., A. I. P. M. de Kroon, and Ben de Kruijff. 1996. Transmembrane movement of phosphatidylcholine in mitochondrial outer membrane vesicles. J. Biol. Chem. 271:11879-11883.
9. Fellmann, P., A. Zachowski, and P. F. Devaux. 1994. Synthesis and use of spin-labeled lipids for studies of the transmembrane movement of phospholipids. In Methods in Molecular Biology. J. M. Graham and J. A. Higgins, editors. Humana Press, Totowa, NJ. 161-175.
10. Haest, C. W. M., G. Plasa, and B. Deuticke. 1981. Selective removal of lipids from the outer membrane layer of human erythrocytes without hemolysis. Consequences for bilayer stability and cell shape. Biochim. Biophys. Acta. 649:701-708.
11. King, M. D., and D. Marsh. 1987. Head group and chain length dependence of phospholipid self-assembly studied by spin-label electron spin resonance. Biochemistry. 26:1224-1231.
12. Klimes, N., G. Lassmann, and B. Ebert. 1980. Time-resolved electron paramagnetic resonance spectroscopy. Stopped-flow EPR apparatus for biological applications. J. Magn. Res. 37:3-99.
13. Kornberg, R. D., and H. M. McConnell. 1971. Inside-outside transitions of phospholipids in vesicle membranes. Biochemistry. 10:1111-1120.
14. Lassmann, G., L. Thelander, and A. Gräslund. 1992. EPR stopped-flow studies of the reaction of the tyrosyl radical of protein R2 from ribonucleotide reductase with hydroxyurea. Biochem. Biophys. Res. Commun. 188:879-887.
15. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
16. Martin, O. C., and R. E. Pagano. 1987. Transbilayer movement of fluorescent analogs of phosphatidylserine and phosphatidylethanolamine at the plasma membrane of cultured cells: evidence for a protein-mediated and ATP-dependent process(es). J. Biol. Chem. 262:5890-5898.
17. Menon, A. K. 1995. Flippases. Trends Cell Biol. 5:355-360.
18. Morrot, G., P. Herve, A. Zachowski, P. Fellmann, and P. F. Devaux. 1989. Aminophospholipid translocase of human erythrocytes: phospholipid substrate specificity and effect of cholesterol. Biochemistry. 28: 3456-3462.
19. Müller, K., T. Pomorski, P. Müller, A. Zachowski, and A. Herrmann. 1994. Protein-dependent translocation of aminophospholipids and asymmetric transbilayer distribution of phospholipids in the plasma membrane of ram sperm cells. Biochemistry. 33:9968-9974.
20. Op den Kamp, J. A. F. 1979. Lipid asymmetry in membranes. Annu. Rev. Biochem. 48:47-71.
21. Pomorski, T., P. Müller, B. Zimmermann, K. Burger, P. F. Devaux, and A. Herrmann. 1996. Transbilayer movement of fluorescent and spin-labeled phospholipids in the plasma membrane of human fibroblasts: a quantitative approach. J. Cell Sci. 109:687-698.
22. Rousselet, A., C. Guthmann, J. Matricon, A. Bienvenue, and P. F. Devaux. 1976. Study of the transverse diffusion of spin labeled phospholipids in biological membranes. Biochim. Biophys. Acta. 426:357-371.
23. Schreier-Muccillo, S., D. Marsh, and I. C. P. Smith. 1976. Monitoring the permeability profile of lipid membranes with spin probes. Arch. Biochem. Biophys. 172:1-11.
24. Seigneuret, M., and P. F. Devaux. 1984. ATP-dependent asymmetric distribution of spin-labeled phospholipids in the erythrocyte membrane: relation to shape changes. Proc. Natl. Acad. Sci. USA. 81:3751-3755.
25. Seigneuret, M., A. Zachowski, A. Herrmann, and P. F. Devaux. 1984. Asymmetric lipid fluidity in human erythrocyte membrane: new spin label evidence. Biochemistry. 23:4271-4275.
26. Thomas, P. D., and M. J. Poznansky. 1989. Curvature and composition-dependent lipid asymmetry in phosphatidylcholine vesicles containing phosphatidylethanolamine and gangliosides. Biochim. Biophys. Acta. 978:85-90.
27. Wimalasena, K., S. Dharmasena, and D. S. Wimalasena. 1994. Ascorbate based novel high affinity alternate reductants and competitive inhibitors of dopamine β-monooxygenase. Biochem. Biophys. Res. Commun. 200: 113-119.
28. Wu, G., and W. L. Hubbell. 1993. Phospholipid asymmetry and transmembrane diffusion in photoreceptor disc membranes. Biochemistry. 32: 879-888.
29. Zachowski, A. 1993. Phospholipids in animal eukaryotic membranes: transverse asymmetry and movement. Biochem. J. 294:1-14.
30. Zachowski, A., E. Favre, C. Cribier, F. Galacteros, and P. F. Devaux. 1986. Outside-inside translocation of aminophospholipids in the human erythrocyte membrane is mediated by a specific enzyme. Biochemistry. 25:2585-2590.
31. Zachowski, A., A. Herrmann, A. Paraf, and P. F. Devaux. 1987. Phospholipid outside-inside translocation in lymphocyte plasma membranes is a protein-mediated phenomenon. Biochim. Biophys. Acta. 897:197-200.