A single tube two compartment reverse transcription polymerase chain reaction system for ultrasensitive quantitative detection of hepatitis C virus RNA

Journal of Virological Methods

Volumn 66, Issue 1, 1997, Pages 15-18

  Whitby, K ^a   Garson, J A ^a  

^a ROYAL FREE AND UNIVERSITY COLLEGE MEDICAL SCHOOL   (United Kingdom)

Author keywords

Blood Products; Hepatitis C; Polymerase chain reaction (PCR); Quantification; Screening

Indexed keywords

VIRUS RNA;

ARTICLE; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRUS DETECTION; VIRUS DIAGNOSIS;

HEPACIVIRUS; HUMANS; POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

HEPATITIS C VIRUS;

EID: 0030849907     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(97)00034-7     Document Type: Article

References (8)

1. Brillanti, S., Garson, J.A., Tuke, P.W., Ring, C., Briggs, M., Masci, C., Miglioli, M., Barbara, L., Tedder, R.S. (1991) Effect of α-interferon therapy on hepatitis C viraemia in community-acquired chronic non-A, non-B hepatitis: A quantitative polymerase chain reaction study. J. Med. Virol. 34, 136-141.
2. Chomczynski, P. and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
3. Chou, Q., Russell, M., Birch, D.E., Raymond, J., Bloch, W. (1992) Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications. Nucleic Acids Res. 20, 1717-23.
4. Gomperts, E.D., (1994) HCV and Gammagard in France. Lancet 344, 201.
5. Saldanha, J. and Minor, P. (1996) Collaborative study to assess the suitability of an HCV RNA reference sample for detection of an HCV RNA in plasma pools by PCR. Vox Sanguinis 70, 148-151.
6. Simmonds, P., Zhang, L.Q., Watson, H.G., Rebus, S., Ferguson, E. D., Balfe, P., Leadbetter, G,H., Yap, P,L., Peutherer, J.F., Ludlam, C.A. (1990) Hepatitis C quantification and sequencing in blood products, haemophiliacs, and drug users. Lancet 336, 1469-1472.
7. Tilston, P. and Corbitt, G (1995) A single tube nested PCR for the detection of hepatitis C virus RNA. J. Virol. Methods 53, 121-129.
8. Whitby, K. and Garson, J.A. (1995) Optimisation and evaluation of a quantitative chemiluminescent polymerase chain reaction assay for hepatitis C virus RNA. J. Virol. Methods 51, 75-88.