Basis of the 1:1 stoichiometry of the high affinity receptor FcεRI-IgE complex

European Biophysics Journal

Volumn 25, Issue 5-6, 1997, Pages 471-476

  Keown, M B ^a   Ghirlando, R ^b,c   Mackay, G A ^a   Sutton, B J ^a   Gould, H J ^a  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^c CNRS   (France)

Author keywords

Analytical centrifugation; Fc RI; IgE; Stoichiometry

Indexed keywords

IMMUNOGLOBULIN E RECEPTOR; IMMUNOGLOBULIN FC FRAGMENT;

ARTICLE; BINDING SITE; CRYSTAL STRUCTURE; IMMUNOGLOBULIN STRUCTURE; MOLECULAR WEIGHT; PROTEIN DOMAIN; RECEPTOR AFFINITY; RECEPTOR BINDING; STOICHIOMETRY; TITRIMETRY;

BINDING SITES; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOGLOBULIN E; IMMUNOGLOBULIN EPSILON-CHAINS; IMMUNOGLOBULIN FC FRAGMENTS; KINETICS; MACROMOLECULAR SUBSTANCES; MODELS, STRUCTURAL; MOLECULAR WEIGHT; RECEPTORS, IGE; ULTRACENTRIFUGATION;

EID: 0030849786     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002490050062     Document Type: Article

References (20)

1. Basu M, Hakimi J, Dharm E, Kondas JA, Tsien W-H, Pilson RS, Lin P, Gilfillan A, Haring P, Braswell EH, Nettleton MY, Kochan JP (1993) Purification and characterization of human recombinant IgE-Fc fragments that bind to the human high affinity IgE receptor. J Biol Chem 268:13118-13127
2. Beavil AJ (1996) Structural studies of immunoglobulin E and its receptors. PhD Thesis, London University
3. Beavil AJ, Beavil RL (1997) Automated hydrodynamic modelling of a complex between a human IgE fragment Fcε3-4 and the IgE high affinity receptor FcεRI α-chain. Eur Biophys J 25:463-469
4. Beavil AJ, Young RJ, Sutton BJ, Perkins SJ (1995) Bent domain structure of recombinant human IgE-Fc in solution by X-ray and neutron scattering in conjunction with an automated curve fitting procedure. Biochemistry 34:14449-14461
5. Blank U, Ra CS, Kinet J-P (1991) Characterization of truncated α chain products from human, rat and mouse high affinity receptor for immunoglobulin E. J Biol Chem 266:2639-2646
6. Diesenhofer J (1981) Crystallographic refinement and atomic models of a human Fc fragment and its complex with fragment B of protein A from Staphylococcus aureus at 2.9- and 2.8 Å resolution. Biochemistry 20:2361-2370
7. Durschlag H (1986) In: Hinz HJ (ed) Thermodynamic data for biochemistry and biotechnology. Springer, Berlin Heidelberg New York, pp 45-128
8. Ghirlando R, Keown MB, Mackay GA, Lewis MS, Unkeless JC, Gould HJ (1995) Stoichiometry and thermodynamics of the interaction between the Fc fragment of human IgG1 and its low-affinity receptor FcγRIII. Biochemistry 34:13320-13327
9. Hakimi J, Seals C, Kondas JA, Pettine L, Danho W, Kochan J (1990) The α subunit of the human IgE receptor (FcεRI) is sufficient for high affinity IgE binding. J Biol Chem 265:22079-22081
10. Helm BA, Ling Y, Teale C, Padlan EA, Bruggeman M (1991) The nature and importance of the inter-ε-chain disulphide bonds in human IgE. Eur J Immunol 21:1543-1548
11. Keown MB (1997) Structural studies of the IgE/sFcεPIα complex. PhD Thesis, London University
12. Keown MB, Ghirlando R, Young RJ, Beavil AJ, Owens RJ, Perkins SJ, Sutton BJ, Gould HJ (1995) Hydrodynamic studies of a complex between the Fc fragment of human IgE and a soluble fragment of the FcεRIα-chain. Proc Natl Acad Sci USA 92:1841-1845
13. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685
14. Padlan EA, Davies DR (1986) A model of the Fc of immunoglobulin E. Mol Immunol 23:1063-1075
15. Perkins SJ (1986) Protein volumes and hydration effects. The calculation of partial specific volumes, neutron scattering matchpoints and 280 nm absorption coefficients for proteins and glycoproteins from amino acid sequences. Eur J Biochem 157:169-180
16. Robertson MW (1993) Phage and Escherichia coli expression of the human high affinity immunoglobulin E receptor α-subunit ecto-domain. J Biol Chem 268:12786-12743
17. Shi J, Ghirlando R, Beavil RL, Beavil AJ, Keown MB, Young RJ, Owens RJ, Sutton BJ, Gould HJ (1997) Interaction of the low affinity receptor CD23/FcεRII Lectin Domain (sCD23) with the Feε3-4 fragment of IgE. Biochemistry (in press)
18. Sutton BJ, Gould HJ (1993) The human IgE network. Nature 366: 421-428
19. Young RJ, Owens RJ, Mackay GA, Chan CMW, Shi J, Hide M, Francis DM, Henry AJ, Sutton BJ, Gould HJ (1995) Secretion of recombinant human IgE-Fc by mammalian cells and biological activity of glycosylation site mutants. Protein Engineering 8:193-199
20. Zheng Y, Shopes B, Holowka D, Baird B (1991) Conformations of IgE bound to its receptor FcεRI and in solution. Biochemistry 30:9125-9132