Kinetic assay of serum and urine for urea with use of urease and leucine dehydrogenase

Clinical Chemistry

Volumn 43, Issue 10, 1997, Pages 1932-1936

  Morishita, Yoshitaka ^a   Nakane, Kiyoshi ^a   Fukatsu, Toshiaki ^a   Nakashima, Nobuo ^a   Tsuji, Katsumi ^b   Soya, Yoshihiro ^b   Yoneda, Keizo ^b   Asano, Shigeki ^b   Kawamura, Yoshihisa ^b  

^a NAGOYA UNIVERSITY HOSPITAL   (Japan)

^b TOYOBO CO LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AMMONIA; ASCORBIC ACID; BILIRUBIN; HEMOGLOBIN; INTRALIPID; LEUCINE DEHYDROGENASE; UREA; UREASE;

ARTICLE; BLOOD ANALYSIS; BLOOD LEVEL; ENZYMATIC ASSAY; ENZYME KINETICS; URINALYSIS; URINE LEVEL;

EID: 0030846696     PISSN: 00099147     EISSN: None     Source Type: Journal    
DOI: 10.1093/clinchem/43.10.1932     Document Type: Article

References (6)

1. Hallett CJ, Cook JGH. Produced nicotinamide adenine dinucleotide-coupled reaction for emergency blood urea estimation. Clin Chim Acta 1971;35:33-7.
2. Sampson EJ, Baird MA. Chemical inhibition used in a kinetic urease glutamate dehydrogenase method for urea in serum. Clin Chem 1979;25:1721-9.
3. Marui Y, Nakano T, Hayashi T, Takagahara I. Method of terminating isocitrate dehydrogenase reaction in analytical system. US patent 4,742,001, 1988.
4. Fossati P. Enzymatic urea assay. US patent 4,608,335, 1986.
5. Tsuji K, Soya Y, Yoneda K, Asano S, Kawamura Y, Morishita Y, et al. A novel method for determination of urea nitrogen without interfering endogenous ammonia. Jpn J Clin Chem 1996;25(Suppl 3):29c.
6. Morishita Y, Fukatsu T, Nakane K, Tsuji K, Soga Y, Yoneta K, et al. Determination of urea-nitrogen in serum and urine by urease-leucine dehydrogenase-NADH system. Jpn J Clin Chem 1996; 25(Suppl 3):30c.