Purification and nucleic-acid-binding properties of a Saccharomyces cerevisiae protein involved in the control of ploidy

European Journal of Biochemistry

Volumn 249, Issue 1, 1997, Pages 309-317

  Weber, Viktoria ^a   Wernitznig, Andreas ^a   Hager, Gudrun ^a   Harata, Masahiko ^b   Frank, Peter ^a   Wintersberger, Ulrike ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b TOHOKU UNIVERSITY   (Japan)

Author keywords

KH domain; Nucleic acid binding protein; RNA transport

Indexed keywords

RNA BINDING PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CAENORHABDITIS ELEGANS; CELL FRACTIONATION; DELETION MUTANT; IMMUNOBLOTTING; MOLECULAR WEIGHT; PHENOTYPE; PLOIDY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DOMAIN; PROTEIN PURIFICATION; RNA TRANSPORT; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY;

CAENORHABDITIS ELEGANS; NES; SACCHAROMYCES CEREVISIAE; VERTEBRATA;

EID: 0030843052     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00309.x     Document Type: Article

References (41)

1. Burd, C. G. & Dreyfuss, G. (1994) Conserved structures and diversity of functions of RNA-binding proteins, Science 265, 615-621.
2. Kiledjian, M., Burd, C. G., Görlach, M., Portman, D. S. & Dreyfuss, G. (1994) Structure and function of hnRNP proteins, in RNA-protein interactions (Nagai, K. & Mattaj, I. W., eds) pp. 127-149, Oxford University Press, New York.
3. Siomi, H., Matunis, M. J., Michael, W. M. & Dreyfuss, G. (1993) The pre-mRNA binding K protein contains a novel evolutionarily conserved motif, Nucleic Acids Res. 21, 1193-1198.
4. Castiglione Morelli, M. A., Stier, G., Gibson, T., Joseph, C., Musco, G., Pastore, A. & Travè, G. (1995) The KH module has an αβ fold, FEBS Lett. 358, 193-198.
5. Musco, G., Stier, G., Joseph, C., Castiglione Morelli, M. A., Nilges, M., Gibson, T. & Pastore, A. (1996). Three-dimensional structure and stability of the KH domain: molecular insights into the fragile X syndrome, Cell 85, 237-245.
6. Régnier, P., Grunberg-Manago, M. & Portier, C. (1987) Nucleotide sequence of the pnp gene of Escherichia coli encoding polynucleotide phosphorylase, J. Biol. Chem. 262, 63-68.
7. Gibson, T. J., Thompson, J. D. & Heringa, J. (1993) The KH domain occurs in a diverse set of RNA-binding proteins that include the antiterminator NusA and is probably involved in binding to nucleic acid, FEBS Lett. 324, 361-366.
8. Engebrecht, J. & Roeder, G. S. (1990) MERI, a yeast gene required for chromosome pairing and genetic recombination, is induced in meiosis, Mol. Cell. Biol. 10, 2379-2389.
9. Urlaub, H., Kruft, V., Bischof, O., Müller, E.-C. & Wittmann-Liebold, B. (1995) Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies, EMBO J. 14, 4578-4588.
10. Spiridonova, V. A., Akhmanova, A. S., Kagramanova, V. K., Kopke, A. K. & Mankin, A. S. (1989) Ribosomal protein gene cluster of Halobacterium halobium: nucleotide sequence of the genes coding for S3 and L29 equivalent ribosomal proteins, Can. J. Microbiol. 35, 153-159.
11. Hahm, K., Kim, G., Turck, C. W. & Smale, S. T. (1993) Isolation of a murine gene encoding a nucleic acid-binding protein with homology to hnRNP K, Nucleic Acids Res. 21, 3894.
12. Wong, G., Müller, O., Clark, R., Conroy, L., Moran, M. F., Polakis, P. & McCormick, F. (1992) Molecular cloning and nucleic acid binding properties of the GAP-associated tyrosine phosphoprotein p62, Cell 69, 551-558.
13. Leffers, H., Dejgaard, K. & Celis, J. E. (1995) Characterisation of two major cellular poly(rC)-binding human proteins, each containing three K-homologous (KH) domains, Eur. J. Biochem. 230, 447-453.
14. Kiledjian, M., Wang, X. & Liebhaber, S. A. (1995) Identification of two KH domain proteins in the α-globin mRNP stability complex, EMBO J. 14, 4357-4364.
15. Siomi, H., Siomi, M. C., Nussbaum, R. L. & Dreyfuss, G. (1993) The protein product of the fragile X gene, FMR 1, has characteristics of an RNA-binding protein, Cell 74, 291-298.
16. Siomi, H., Choi, M., Siomi, M. C., Nussbaum, R. L. & Dreyfuss, G. (1994) Essential role for KH domains in RNA binding: impaired RNA binding by a mutation in the KH domain of FMR1 that causes fragile X syndrome, Cell 77, 33-39.
17. Zhang, Y., O'Connor, J. P., Siomi, M. C., Srinivasan, S., Dutra, A., Nussbaum, R. L. & Dreyfuss, G. (1995) The fragile X mental retardation syndrome protein interacts with novel homologs FXR1 and FXR2, EMBO J. 14, 5358-5366.
18. Schmidt, C., Henkel, B., Pöschl, E., Zorbas, H., Purschke, W. G., Gloe, T. R. & Müller, P. K. (1992) Complete cDNA sequence of chicken vigilin, a novel protein with amplified and evolutionary conserved domains, Eur. J. Biochem. 206, 625-634.
19. Plenz, G., Kügler, S., Schnittger, S., Rieder, H., Fonatsch, C. & Müller, P. K. (1994) The human vigilin gene: identification, chromosomal localization and expression pattern, Hum. Genet. 93, 575-582.
20. McKnight, G. L., Reasoner, J., Gilbert, T., Sundquist, K. O., Hokland, B., McKernan, P. A., Champagne, J., Johnson, C. J., Bailey, M. C., Holly, R., O'Hara, P. J. & Oram, J. F. (1992) Cloning and expression of a cellular high density lipoprotein-binding protein that is up-regulated by cholesterol loading of cells, J. Biol. Chem. 267, 12 131-12 141.
21. Wintersberger, U., Kühne, C. & Karwan, A. (1995) Scp160p, a new yeast protein associated with the nuclear membrane and the endoplasmic reticulum, is necessary for maintainance of exact ploidy, Yeast 11, 929-944.
22. Delahodde, A., Becam, A. M., Perea, J. & Jacq, C. (1986) A yeast protein HX has homologies with the histone H2AF expressed in chicken embryo, Nucleic Acids Res. 14, 9213-9214.
23. Sherman, F. (1991) Getting started with yeast, in Guide to yeast genetics and molecular biology (Guthrie, C. & Fink, G. R., eds), pp. 3-21, Academic Press, New York.
24. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254.
25. Merril, C. R., Goldman, D. & VanKeuren, M. L. (1984) Gel protein stains: silver stain, Methods Enzymol. 104, 441-447.
26. Weber, V., Harata, M., Hauser, H. & Wintersberger, U. (1995) The actin-related protein Act3p of Saccharomyces cerevisiae is located in the nucleus, Mol. Biol. Cell 6, 1263-1270.
27. Tibbetts, M., Donovan, M., Roe, S., McCabe Stiltner, A. & Hammond, C. I. (1994) KIN1 and KIN2 protein kinases localize to the cytoplasmic face of the yeast plasma membrane, Exp. Cell Res. 213, 93-99.
28. Deshmukh, M., Tsay, Y.-F., Paulovich, A. G. & Woolford, J. L. (1993) Yeast ribosomal protein L1 is recquired for the stability of newly synthesized 5S rRNA and the assembly of 60S ribosomal subunits, Mol. Cell. Biol. 13, 2835-2845.
29. Karwan, R., Blutsch, H. & Wintersberger, U. (1983) Physical association of a DNA polymerase stimulating activity with a ribonuclease H purified from yeast, Biochemistry 22, 5500-5507.
30. Fischer, U., Huber, J., Boelens, W. C., Mattaj, I. W. & Lührmann, R. (1995) The HIV-1 rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs, Cell 82, 475-483.
31. Fridell, R. A., Benson, R. E., Hua, J., Bogerd, H. P. & Cullen, B. R. (1996). A nuclear role for the fragile X mental retardation protein, EMBO J. 15, 5408-5414.
32. Bogerd, H. P., Fridell, R. A., Benson, R. E., Hua, J. & Cullen, B. R. (1996) Protein sequence requirements for function of the human T-cell leukemia virus type 1 Rex nuclear export signal delineated by a novel in vivo randomization-selection assay, Mol. Cell. Biol. 16, 4207-4214.
33. Kügler, S., Grünweller, A., Probst, C., Klinger, M., Müller, P. K. & Kruse, C. (1996) Vigilin contains a functional nuclear localization sequence and is present in both the cytoplasm and the nucleus, FEBS Lett. 382, 330-334.
34. Hager, G. (1995) Characterization and localization of Scp160p from Saccharomyces cerevisiae, Diploma thesis, University of Agriculture, Vienna.
35. Kiledjian, M. & Dreyfuss, G. (1992) Primary structure and binding activity of the hnRNP U protein: binding RNA through RGG box, EMBO J. 11, 2655-2664.
36. Swanson, M. S., Nakagawa, T. Y., LeVan, K. & Dreyfuss, G. (1987) Primary structure of human ribonucleoprotein particle C proteins: conservation of sequence and domain structures in heterogeneous nuclear RNA, mRNA, and pre-rRNA-binding proteins, Mol. Cell. Biol. 7, 1731-1739.
37. Neu-Yilik, G., Zorbas, H., Gloe, T. R., Raabe, H.-M., Hopp-Christensen, T. A. & Müller, P. K. (1993) Vigilin is a cytoplasmic protein. A study on its expression in primary cells and in established cell lines of different species, Eur. J. Biochem. 213, 727-736.
38. Dejgaard, K. & Leffers, H. (1996) Characterisation of the nucleic acid binding activity of KH domains. Different properties of different domains, Eur. J. Biochem. 241, 425-431.
39. Siomi, M., Zhang, Y., Siomi, H. & Dreyfuss, G. (1996) Specific sequences in the fragile X syndrome protein FMR1 and the FXR proteins mediate their binding to 60S ribosomal subunits and the interactions among them, Mol. Cell. Biol. 16, 3825-3832.
40. Adelman, M. R., Sabatini, D. D. & Blobel, G. (1973) Ribosome-membrane interaction, J. Cell Biol. 56, 206-229.
41. Thompson, J. D., Higgins, D. G. & Gibson T. J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice, Nucleic Acids Res. 22, 4673-4680.