Quantitative PCR analysis of TNF-α and IL-1β mRNA levels in pediatric IBD mucosal biopsies

Digestive Diseases and Sciences

Volumn 42, Issue 7, 1997, Pages 1557-1566

  Dionne, S ^b   Hiscott, J ^b   D'Agata, I ^b   Duhaime, Anne ^b   Seidman, E G ^a  

^a UNIVERSITY OF MONTREAL   (Canada)

^b NONE

Author keywords

Crohn's disease, ulcerative colitis; Inflammatory bowel disease; Interleukin 1; PCR; Tumor necrosis factor

Indexed keywords

INTERLEUKIN 1BETA; MESSENGER RNA; TUMOR NECROSIS FACTOR ALPHA;

ADOLESCENT; ADULT; ARTICLE; CHILD; CLINICAL ARTICLE; COLON BIOPSY; CONTROLLED STUDY; CROHN DISEASE; ENTERITIS; FEMALE; GENE ACTIVITY; GENE EXPRESSION; HUMAN; HUMAN TISSUE; IMMUNOCOMPETENT CELL; INFLAMMATION; MALE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA TRANSCRIPTION; ULCERATIVE COLITIS;

ADOLESCENT; BIOPSY; CASE-CONTROL STUDIES; CHILD; COLITIS; COLITIS, ULCERATIVE; COLON; CROHN DISEASE; EOSINOPHILIA; FEMALE; GENE EXPRESSION; HUMANS; INTERLEUKIN-1; INTESTINAL MUCOSA; MALE; POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR NECROSIS FACTOR-ALPHA;

EID: 0030842468     PISSN: 01632116     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018895500721     Document Type: Article

References (61)

1. Strober W, Ehrhardt RO: Chronic intestinal inflammation: An unexpected outcome in cytokine or T cell receptor mutant mice. Cell 75:203-205, 1993
2. Podolsky DK: Inflammatory bowel disease. N Engl J Med 325:928-937, 1991
3. Seidman E: Epidemiology and current etiological concepts. In Pediatric Inflammatory Bowel Disease. J Levine (ed). Philadelphia, Lippincott, 1994, pp 1-14
4. Braegger CP: Immunopathogenesis of chronic inflammatory bowel disease. Acta Paediatr 395:18-21, 1994
5. Schreiber S, Raedler A, Stenson WF, MacDermott RP: The role of mucosal immune system in inflammatory bowel disease. Gastroenterol Clin North Am 21:451-502, 1992
6. Sartor RB: Current concepts of the etiology and pathogenesis of ulcerative colitis and Crohn's disease. Gastroenterol Clin North Am 24:475-507, 1995
7. Sadlack B, Merz H, Schorle H, Schimpl A, Feller AC, Horak I: Ulcerative colitis-like disease in mice with disrupted interleukin-2 gene. Cell 75:253-261, 1993
8. Kühn R, Löhler J, Rennick D, Rajewsky K, Müller W: Interleukin-10 deficient mice develop chronic enterocolitis. Cell 75:263-274, 1993
9. Sartor B: Cytokines in intestinal inflammation: Pathophysiological and clinical considerations. Gastroenterology 106:533-539, 1994
10. McCabe RP, Secrist H, Botney M, Egan M, Peters MG: Cytokine mRNA expression in intestine from normal and inflammatory bowel disease patients. Clin Immunol Immunopathol 66:52-58, 1993
11. Brynskov J, Nielsen OH, Ahnfelt-Rønne I, Bendtzen K: Cytokines in inflammatory bowel disease. Scand J Gastroenterol 27:897-906, 1992
12. Beagley KW, Elson CO: Cells and cytokines in mucosal immunity and inflammation. Gastroenterol Clin North Am 21:347-366, 1992
13. Braegger CD, Nicholls S, Murch SH, Stephens S, MacDonald TT: Tumor necrosis factor alpha in stool as a marker of intestinal inflammation. Cancer 339:89-91, 1992
14. Murch SH, Lamkin VA, Savage MO, Walker-Smith JA, MacDonald TT: Serum concentrations of tumor necrosis factor alpha in childhood chronic inflammatory bowel disease. Gut 32:913-917, 1991
15. Hyams J, Treem W, Eddy E, Wyzgz N, Moore R: Tumor necrosis factor alpha is not elevated in children with inflammatory bowel disease. J Pediatr Gastroenterol Nutr 12:233-276, 1991
16. Capello M, Keshav S, Prince C, Jewell DP, Gordon S: Detection of mRNAs for macrophage products in inflammatory bowel disease by in situ hybridization. Gut 33:1214-1219, 1992
17. Stevens C, Walz G, Singaram C, Lipman ML, Zanker B, Muggia A, Antonioli D, Peppercorn MA, Strom TB: Tumor necrosis factor-α, interleukin-1β, and interleukin-6 expression in inflammatory bowel disease. Dig Dis Sci 37:818-826, 1992
18. Isaacs KL, Sartor RB, Haskill S: Cytokine messenger RNA profiles in inflammatory bowel disease mucosa detected by polymerase chain reaction amplification. Gastroenterology 103:1587-1595, 1992
19. Mahida YR, Wu K, Jewell DP: Enhanced production of interleukin-1β by mononuclear cells isolated from mucosa with ulcerative colitis or Crohn's disease. Gut 30:835-838, 1989
20. Ligumski M, Simon DL, Karmeli F, Rachmilewitz D: Role of interleukin 1 in inflammatory bowel disease-enhanced production during active disease. Gut 31:686-689, 1990
21. Mahida YR, Lamming CED, Gallagher A, Hawthorne A, Hawkey CJ: 5-Aminosalicylic acid is a potent inhibitor of interleukin 1β production in organ culture of colonic biopsy specimens from patients with inflammatory bowel disease. Gut 32:50-54, 1991
22. Brynskov J, Trede N, Vilien M, Andersen CB, Bentzen K: Increased concentrations of interleukin 1β, interleukin 2, and soluble interleukin-2 receptors in endoscopical mucosal biopsy specimens with active inflammatory bowel disease. Gut 33:55-58, 1992
23. Youngman KR, Simon PL, West GA, Cominelli F, Rachmilewitz D, Klein JS, Fiocchi C: Localization of intestinal interleukin 1 activity and protein and gene expression to lamina propria cells. Gastroenterology 104:749-758, 1993
24. Reinecker HC, Steffen M, Witthoef T, Pflueger I, Schreiber S, MacDermott RP, Raedler A: Enhanced secretion of tumor necrosis factor-alpha, IL-6, and IL-1β by isolated lamina propria mononuclear cells from patients with ulcerative colitis and Crohn's disease. Clin Exp Immunol 94:174-181, 1993
25. Mullin GE, Lazenby AJ, Harris MC, Bayless TM, James SP: Increased interleukin 2 messenger RNA in the intestinal mucosal lesions of Crohn's disease but not ulcerative colitis. Gastroenterology 102:1620-1627, 1992
26. Izutani R, Ohyanagi H, MacDermott RP: Quantitative PCR for detection of femtogram quantities of interleukin-8 mRNA expression. Microbiol Immunol 38:233-237, 1994
27. Andus T, Targan SR, Deem R, Toyoda H: Measurement of tumor necrosis factor α mRNA in small numbers of cells by quantitative polymerase chain reaction. Reg Immunol 5:11-17, 1993
28. de Dombal FT, Burton IL, Clamp SE, Goligher JC: Short term course and prognosis of Crohn's disease. Gut 15:435-441, 1974
29. Chomczynski P: A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15:532-535, 1993
30. Gurbindo C, Russo P, Sabbah S, Lohoues MJ, Seidman E: Interleukin-2 activity of colonic lamina propria mononuclear cells in a rat model of experimental colitis. Gastroenterology 104:964-972, 1993
31. McCall RD, Haskill S, Zimmermann EM, Lund PK, Thompson RC, Sartor RB: Tissue interleukin 1 and interleukin-1 receptor antagonist expression in enterocolitis in resistant and susceptible rats. Gastroenterology 106:960-972, 1994
32. Siebert PD: Quantitative RT-PCR. Methods and Applications, book 3. Clontech Laboratories, Palo Alto, 1993
33. Innis MA, Gelfand DH: Optimization of PCRs. In PCR Protocols: A guide to methods and applications. MA Innis, DH Gelfand, JJ Sninsky, TJ White (eds). San Diego, Academic Press, 1990, pp 3-12
34. Landgraf A, Wolfes H: Taq polymerase (EC 2.7.7.7.). With particular emphasis on its use in PCR protocols. Methods Mol Biol 16:31-58, 1993
35. Wang AM, Doyle MV, Mark DF: Quantitation of mRNA by the polymerase chain reaction. Proc Natl Acad Sci USA 86:9717-9721, 1989
36. Siebert PD, Larrick JW: Competitive PCR. Nature 359:557-558, 1992
37. He Q, Marjamäki M, Soini H, Mertsola J, Viljanen MK: Primers are decisive for sensitivity of PCR. Biotechniques 17:82-86, 1994
38. Wiesner RJ: Direct quantification of picomolar concentrations of mRNAs by mathematical analysis of a reverse transcription/ exponential polymerase chain reaction assay. Nucleic Acids Res 20:5863-5864, 1992
39. Robinson MO, Simon MI: Determining transcript number using the polymerase chain reaction: PgK-2, mPz, and PGK-2 transgene mRNA levels during spermatogenesis. Nucleic Acids Res 19:1557-1562, 1991
40. Siebert PD, Larrick JW: PCR mimics: Competitive DNA fragment for use as internal standards in quantitative PCR. Biotechniques 14:244-249, 1993
41. Trapnell BC: Quantitative evaluation of gene expression in freshly isolated human respiratory epithelial cells. Am J Physiol 264:L199-L212, 1993
42. Grayson DR, Bovolin P, Santi MR: Absolute quantitation of γ-aminobutyric acid A receptor subunit messenger RNAs by competitive polymerase chain reaction. Methods Neurosci 12:191-207, 1993
43. Becker-André M: Absolute levels of mRNA by polymerase chain reaction-aided transcript titration assay. Methods Enzymol 218:420-445, 1993
44. Scheuermann RH, Bauer SR: Polymerase chain reaction-based mRNA quantitation using an internal standard: analysis of oncogene expression. Methods Enzymol 218:446-473, 1993
45. D'Addario M, Roulston A, Wainberg MA, Hiscott J: Coordinate enhancement of cytokine gene expression in human immunodeficiency virus type 1 infected promonocytic cells. J Virol 64:6080-6089, 1990
46. Kotewicz ML, Sampson CM, D'Alessio JM, Gerard GF: Isolation of cloned moloney murine leukemia virus reverse transcriptase lacking ribonuclease H activity. Nucleic Acids Res 16:265-277, 1988
47. Ma TS, Brink PA, Perryman B, Roberts R: Improved quantification with validation of multiple mRNA species by polymerase chain reaction: Application to human myocardial creatine kinase M and B. Cardiovasc Res 28:464-471, 1994
48. Surawicz CM, Haggitt RC, Husseman M, McFarland LV: Mucosal biopsy diagnosis of colitis: acute self-limited colitis and idiopathic inflammatory bowel disease. Gastroenterology 107:755-763, 1994
49. Shanahan F: Pathogenesis of ulcerative colitis. Lancet 342:407-411, 1993
50. Breese E, Braegger CP, Corrigan CJ, Walker-Smith JA, MacDonald TT: Interleukin-2 and interferon-γ secreting T cells in normal and diseased human intestinal mucosa. Immunology 78:127-131, 1993
51. Lionetti P, Breese E, Braegger CP, Murch SH, Taylor J, MacDonald TT: T-cell activation can induce either mucosal destruction or adaptation in cultured human fetal small intestine. Gastroenterology 105:373-381, 1993
52. Mahida YR: The role of macrophages in the pathogenesis of inflammatory bowel disease. Can J Gastroenterol 7:83-86, 1993
53. Weldon MJ, Maxwell JD: Lymphocyte and macrophage interleukin receptors in inflammatory bowel disease: A more selective target for therapy? Gut 35:867-871, 1994
54. Thomas TK, Will PC, Srivastava A: Evaluation of an interleukin-1 receptor antagonist in the rat acetic acid-induced colitis model. Agents Actions 34:187-190, 1991
55. Cominelli F, Nast CC, Duchini A, Lee M: Recombinant interleukin-1 receptor antagonist blocks the proinflammatory activity of endogenous interleukin-1 in rabbit immune colitis. Gastroenterology 103:65-71, 1992
56. Mazlam MZ, Hodgson HSF: Interrelations between interleukin-6, interleukin-1β, plasma C-reactive protein values, and in vitro C-reactive protein generation in patients with inflammatory bowel disease. Gut 35:177-183, 1994
57. Olson AD, Ayass M, Chensue S: Tumor necrosis factor and IL-1β expression in pediatric patients with inflammatory bowel disease. J Pediatr Gastroenterol Nutr 16:241-246, 1993
58. Murch SH, Braegger CP, Walker-Smith J, MacDonald TT: Location of tumor necrosis factor α by immunohistochemistry in chronic inflammatory bowel disease. Gut 34:1705-1709, 1993
59. Breese EJ, Michie CA, Nicholls SW, Murch SH, Williams CB, Domizio P, Walker-Smith JA, MacDonald TT: Tumor necrosis factor α-producing cells in the intestinal mucosa of children with inflammatory bowel disease. Gastroenterology 106:1455-1466, 1994
60. Plevy SE, Targan SR, Deem RL, Toyoda H: Increased mucosal TNFα mRNA levels and numbers of TNFα producing cells are unique to Crohn's disease mucosal inflammation. Gastroenterology 106:A754, 1994
61. Schreiber S, Koop I, Bauditz J, Nikolaus S, Lochs H, Raedler A: Increased secretion of pro-inflammatory cytokines by LPMNC is a predictor for relapse of IBD. Gastroenterology 108:A913, 1995