The signal transducer gp130: Bacterial expression, refolding and properties of the carboxy-terminal domain of the cytokine-binding module

European Journal of Biochemistry

Volumn 247, Issue 1, 1997, Pages 425-431

  Müller Newen, Gerhard ^a   Pflanz, Stefan ^a   Hassiepen, Ulrich ^a   Stahl, Jürgen ^a   Wollmer, Axel ^a   Heinrich, Peter C ^a   Grötzinger, Joachim ^a  

^a RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Circular dichroism; Fluorescence spectroscopy; Folding; Gp130 domain; NMR

Indexed keywords

CYTOKINE; GLYCOPROTEIN GP 130;

ARTICLE; CIRCULAR DICHROISM; ESCHERICHIA COLI; FLUORESCENCE SPECTROSCOPY; NONHUMAN; NUCLEAR MAGNETIC RESONANCE; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN FOLDING; SIGNAL TRANSDUCTION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030841126     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00425.x     Document Type: Article

References (26)

1. Kishimoto, T., Akira, S., Narazaki, M. & Taga, T. (1995) Interleukin-6 family of cytokines and gp130. Blood 86, 1243-1254.
2. Yamasaki, K., Taga, T., Hirata, Y., Yawata, H., Kawanishi, Y., Seed, B., Taniguchi, T., Hirano, T. & Kishimoto, T. (1988) Cloning and expression of the human interleukin-6 (BSF-2/IFN β 2) receptor. Science 241, 825-828.
3. Davis, S., Aldrich, T. H., Valenzuela, D. M., Wong, V. V., Furth, M. E., Squinto, S. P. & Yancopoulos, G. D. (1991) The receptor for ciliary neurotrophic factor. Science 253, 59-63.
4. Hilton, D. J., Hilton, A. A., Raicevic, A., Rakar, S., Harrison, S. M., Gough, N. M., Begley, C. G., Metcalf, D., Nicola, N. A. & Willson, T. A. (1994) Cloning of a murine IL-11 receptor α-chain; requirement for gp130 for high affinity binding and signal transduction. EMBO J. 13, 4765-4775.
5. Cherel, M., Sorel, M., Leheau, B., Dubois, S., Moreau, J. F., Bataille, R., Minvielle, S. & Jacques, Y. (1995) Molecular cloning of two isoforms of a receptor for the human hematopoietic cytokine interleukin-11, Blood 86, 2534-2540.
6. Murakami, M., Hibi, M., Nakagawa, N., Nakawaga, T., Yasukawa, K., Yumanishi, K., Taga, T. & Kishimoto, T. (1993) IL-6-induced homodimerization of gp130 and associated activation of a tyrosine kinase, Science 260, 1808-1810.
7. Lütticken, C., Wegenka, U. M., Yuan, J., Buschmann, J., Schindler, C., Ziemiecki, A., Harpur, A. G., Wilks, A. F., Yasukawa, K., Taga, T. Kishimoto, T., Barbieri, G., Pellegrini, S., Sendtner, M., Heinrich, P. C. & Horn, F. (1994) Association of transcription factor APRF and protein kinase JAK1 with the interleukin-6 signal transducer gp130, Science 263, 89-92.
8. Hibi, M., Murakami, M., Saito, M., Hirano, T., Taga, T. & Kishimoto, T. (1990) Molecular cloning and expression of an IL-6 signal transducer, gp130, Cell 63, 1149-1157.
9. Bazan, J. F. (1990) Structural design and molecular evolution of a cytokine receptor superfamily. Proc. Natl Acad. Sci. USA 87, 6934-6938.
10. Yawata, H., Yasukawa, K., Natsuka, S., Murakami, M., Yamasaki, K., Hibi. M., Taga, T. & Kishimoto, T. (1993) Structure-function analysis of human IL-6 receptor: dissociation of amino acid residues required for IL-6-binding and for IL-6 signal transduction through gp130, EMBO J. 12, 1705-1712.
11. Anaguchi, H., Hiraoka, O., Yamasaki, K., Naito, S. & Ota, Y. (1995) Ligand binding characteristics of the carboxyl-terminal domain of the cytokine receptor homologous region of the granulocyte colony-stimulating factor receptor, J. Biol. Chem. 270, 27 845-27 851.
12. Somers, W., Ultsch, M., De Vos, A. M. & Kossiakoff, A. A. (1994) The X-ray structure of a growth hormone-prolactin receptor complex. Nature 372, 478-481.
13. Livnah, O., Stura, E. A., Johnson, D. L., Middleton, S. A., Mulcahy, L. S., Wrighton, N. C., Dower, W. J., Jolliffe, L. K. & Wilson, I. A. (1996) Functional mimicry of a protein hormone by a peptide agonist: The EPO receptor complex at 2.8 Å, Science 273, 464-471.
14. Horsten, U., Schmitz-Van de Leur, H., Müllberg, J., Heinrich, P. C. & Rose-John, S. (1995) The membrane distal half of gp130 is responsible for the formation of a ternary complex with IL-6 and the IL-6 receptor, FEBS Lett. 360, 43-46.
15. Studier, F. W., Rosenberg, A. H., Dunn, J. J. & Dubendorff, J. W. (1990) Use of T7 RNA polymerase to direct expression of cloned genes, Methods Enzymol. 185, 60-89.
16. Chen, G. C. & Yang, J. T. (1977) Two-point calibration of circular dichrometer with d-10-camphorsulfonic acid, Anal. Lett. 10, 1195-1207.
17. Bucci, E. & Steiner, R. F. (1988) Anisotropy decay of fluorescence as an experimental approach to protein dynamics, Biophys. Cliem. 30, 199-224.
18. Szabo, A. G. & Rayner, D. M. (1980) Fluorescence decay of tryptophan conformers in aqueous solution, J. Am. Chem. Soc. 102, 554-563.
19. Waxman, E., Rusinova, E., Hasselbacher, C. A., Schwartz, G. P., Laws, W. R. & Ross, J. B. (1993) Determination of the tryptophan:tyrosine ratio in proteins, Anal. Biochem. 210, 425-428.
20. Grötzinger, J., Kurapkat, G., Wollmer, A., Kalai, M. & Rose-John, S. (1997) The family of the IL-6-type cytokines: Specificity and promiscuity of the receptor complexes, Proteins Struct. Funct. Genet. 27, 96-109.
21. Grishina, I. B. & Woody, R. W. (1994) Contributions of tryptophan side chains to the circular dichroism of globular proteins: exciton couplets and coupled oscillators, Faraday Discuss., 245-262.
22. Sreerama, N. & Woody, R. W. (1994) Protein secondary structure from circular dichroism spectroscopy. Combining variable selection principle and cluster analysis with neural network, ridge regression and self-consistent methods, J. Mol. Biol. 242, 497-507.
23. Wüthrich, K. (1986) NMR of proteins and nucleic acids. John Wiley & Sons, New York.
24. Burstein, E. A., Vedenkina, N. S. & Ivkova, M. N. (1973) Fluorescence and the location of tryptophan residues in protein molecules, Photochem. Photobiol. 18, 263-279.
25. Leenders, H. R., van Vervoort, J. H. A. & Visser, A. J. (1990) Time-resolved fluorescence studies of flavodoxin. Fluorescence decay and fluorescence anisotropy decay of tryptophan in Desulfovibrio flavodoxins, Eur. Biophys. J. 18, 43-55.
26. Gerhartz, C., Dittrich, E., Stoyan, T., Rose-John, S., Yasukawa, K., Heinrich, P. C. & Graeve, L. (1994) Biosynthesis and half-life of the interleukin-6 receptor and its signal transducer gp130. Eur. J. Biochem. 223, 265-274.