Immunodiagnosis of human leptospirosis by dot-ELISA for the detection of IgM, IgG, and IgA antibodies

American Journal of Tropical Medicine and Hygiene

Volumn 56, Issue 6, 1997, Pages 650-655

  Da Silva, Marcos Vinicius ^a   Nakamura, Paulo Mutuko ^b   Camargo, Eide Dias ^b   Batista, Luiza ^a   Vaz, Adelaide Jose ^b   Romero, Eliete Calo ^b   Brandao, Angela Pires ^b  

^a INSTITUTO DE INFECTOLOGIA EMÍLIO RIBAS   (Brazil)

^b INSTITUTO ADOLFO LUTZ   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIUM ANTIBODY; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; IMMUNOGLOBULIN M;

ADOLESCENT; ADULT; ANTIBODY DETECTION; ARTICLE; CHILD; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; COST; DIAGNOSTIC ACCURACY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; LEPTOSPIRA INTERROGANS; LEPTOSPIROSIS; MAJOR CLINICAL STUDY; MALE; SCHOOL CHILD;

BACTERIA (MICROORGANISMS); LEPTOSPIRA INTERROGANS;

EID: 0030840874     PISSN: 00029637     EISSN: None     Source Type: Journal    
DOI: 10.4269/ajtmh.1997.56.650     Document Type: Article

References (13)

1. Faine S, ed., 1982. Guidelines for the Control of Leptospirosis. Geneva: World Health Organization. Publication no. 67.
2. Silva MV, Camargo ED, Vaz AJ, Souza AMC, Chieffi PP, Sakata EE, 1990. Imunodiagnostico da leptospirose humana atraves do teste ELISA-IgM, empregando-se diferentes preparacoes antigenicas a partir de sorotipos prevalentes de Leptospira interrogans. Rev Inst Med Trop Sao Paulo 32: 233-239.
3. Cole JR Jr, Sulzer CR, Pursell AR, 1973. Improved microtechnique for the leptospiral microscopic agglutination test. Appl Microbiol 25: 976-980.
4. Adler B, Murphy AM, Locarnini SA, Faine S, 1980. Detection of specific anti-leptospiral immunoglobulins M and G in human serum by solid-phase enzyme-linked immunosorbent assay. J Clin Microbiol 11: 452-457.
5. Silva MV, Camargo ED, Vaz AJ, Souza AMC, Ueda M, Sakata EE, 1988. Teste imunoenzimatico (ELISA) para deteccao de anticorpos circulantes da classe IgM na leptospirose humana. Rev Inst Med Trop Sao Paulo 30: 95-100.
6. Camargo ED, Silva MV, Vaz AJ, Batista L, Brandao AP, Ferreira AW, Romero EC, Barbosa PRS, 1994. ELISA-IgM applied to cerebrospinal fluid in human leptospirosis. Serodiagn Immunother Infect Dis 6: 19-22.
7. Bradford MM, 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing principle of protein dye binding. Anal Biochem 72: 248-254.
8. Camargo ED, Nakamura PM, Vaz AJ, Silva MV, Chieffi PP, Melo EO, 1992. Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparison of the assay with ELISA. Rev Inst Med Trop Sao Paulo 34: 55-60.
9. Thiermann AB, Garret LA, 1983. Enzyme-linked immunosorbent assay for the detection of antibodies to Leptospira interrogans serovar hardjo and pomona in cattle. Am J Vet Res 44: 884-887.
10. Pappas MG, Ballow RW, Gray MR, Takafuji ET, Miller RN, Hockmeyer WT, 1985. Rapid serodiagnosis of leptospirosis using the IgM specific dot-ELISA: comparison with the microscopic agglutination test. Am J Trop Med Hyg 34: 346-354.
11. Watt G, Alquiza LM, Padre LP, Tuazon ML, Laughlin LW, 1988. The rapid diagnosis of leptospirosis: a prospective comparison of the dot-enzyme linked immunosorbent assay and the genus-specific microscopic agglutination test at different stages of illness. J Infect Dis 157: 840-842.
12. Silva MV, Camargo ED, 1992. Teste imunoenzimatico (ELISA) para deteccao de anticorpos circulantes da classe IgA na leptospirose humana. Rev Inst Med Trop Sao Paulo 34: 239-242.
13. Silva MV, Camargo ED, Batista L, Vaz AJ Brandao AP, Nakamura PM, Negrao JM, 1995. Behaviour of specific IgM, IgG and IgA class antibodies in human leptospirosis during the acute phase of the disease and during convalescence. J Trop Med Hyg 98: 268-272.