Determining isoelectric points of model proteins and Bacillus subtilis neutral protease by the cross partitioning using poly(ethylene glycol)/Dextran aqueous two-phase systems

Colloids and Surfaces B: Biointerfaces

Volumn 9, Issue 3-4, 1997, Pages 131-137

  Han, Jung Hoon ^a,b   Lee, Cherl Ho ^a  

^a Korea University   (South Korea)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Aqueous two phase system; Bacillus subtilis neutral protease; Chromatography; Cross partitioning; Isoelectric pH; Partition coefficient

Indexed keywords

BOVINE SERUM ALBUMIN; LYSOZYME; NEUTRAL PROTEINASE; OVOTRANSFERRIN; SERUM ALBUMIN;

ARTICLE; BACILLUS SUBTILIS; ENZYME PURIFICATION; ION PAIR CHROMATOGRAPHY; ISOELECTRIC POINT; PARTITION COEFFICIENT; PH; PRIORITY JOURNAL;

EID: 0030839768     PISSN: 09277765     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0927-7765(97)00025-8     Document Type: Article

References (29)

1. Å. Albertsson, in H. Walter, D.E. Brooks, D. Fischer (Eds.), Partitioning in Aqueous Two-phase Systems. Theory, Methods, Uses, and Applications to Biotechnology, Academic Press, Orlando, FL, 1985, pp. 1-10.
2. Å. Albertsson, Partition of Cell Particles and Macromolecules, 3rd Edition, Wiley-Interscience, New York, 1986, pp. 8-39.
3. D. Bandyopadhyay, in T.G. Pretlow II, T.G. Pretlow (Eds.), Cell Separation. Methods and Selected Applications, Vol. 4. Academic Press, Orlando, FL, 1987, pp. 171-194.
4. H. Walter, G Johansson, D.E. Brooks, Anal. Biochem. 197 (1991) 1-18.
5. J.H. Han, C.-H. Lee, Colloids Surf. B: Biointerfaces 9 (1997) 109-116.
6. Å. Albertsson, Adv. Protein Chem. 24 (1970) 309-341.
7. Å. Albertsson, B. Andersson, C. Larsson, H.E. Åkerlund, Meth. Biochem. Anal. 28 (1982) 115-150.
8. H. Walter, in N. Catsimpoolas (Ed.), Methods of Cell Separation, Vol. 1, Plenum Press, New York, 1977, pp. 307-354.
9. E. Brooks, K.A. Sharp, D. Fischer, in H. Walter, D.E. Brooks, D. Fischer (Eds.), Partitioning in Aqueous Two-phase Systems. Theory, Methods, Uses, and Applications to Biotechnology, Academic Press, Orlando, FL, 1985, pp. 11-84.
10. H. Walter, E.J. Krob, D.E. Brooks, Biochemistry 15 (14) (1976) 2959-2964.
11. G. Johansson, in H. Walter, G. Johansson ( Eds.), Methods in Enzymology, Vol. 228, Academic Press, San Diego, CA, 1994, pp. 28-42.
12. Å. Albertsson, in D. Fisher, A. Sutherland (Eds.), Separations Using Aqueous Phase Systems, Plenum Press, NY, 1989, pp. 3-5.
13. S. King, in D.S. Soane (Ed.), Polymer Applications for Biotechnology: Macromolecular Separation and Identification, Prentice Hall, Englewood Cliffs, NJ, 1992, pp. 204-243.
14. Å. Albertsson, E.J. Nyns, Arkiv Kemi. 17 (16) (1960) 197-206.
15. G. Johansson, Biochim. Biophys. Acta 221 (1970) 387-390.
16. Y. Zaslavsky, N.M. Mestechkina, S.V. Rogozhin, Biochim. Biophys. Acta 579 (1979) 463-465.
17. Y. Zaslavsky, L.M. Miheeva, N.M. Mestechkina, V.M. Pogorelov, S.V. Rogozhin, FEBS Lett. 94 (1) (1978) 77-80.
18. Å. Albertsson, Partition of Cell Particles and Macromolecules, 3rd Edn, Wiley-Interscience, New York, 1986, pp. 73-111.
19. M. Agarwal, V.G. Gaikar, Bioseparation 4 (1994) 335-342.
20. U. Dissing, B. Mattiason, Bioseparation 3 (1993) 233-240.
21. H. Walter, S. Sasakawa, Biochemistry 10 (1) (1971) 108-113.
22. E. Ericson, Biochim. Biophys. Acta 356 (1974) 100-107.
23. D. Flanagan, in H. Walter, D.E. Brooks, D. Fisher (Eds.), Partitioning in Aqueous Two-phase Systems: Theory, Methods, Uses, and Applications to Biotechnology, Academic Press, Orlando, FL, 1985, pp. 453-495.
24. Å. Albertsson, Partition of Cell Particles and Macromolecules, 3rd Edn, Wiley-Interscience, New York, 1986, pp. 147-172.
25. Å. Albertsson, Partition of Cell Particles and Macromolecules, 3rd Edn, Wiley-Interscience), New York, 1986, pp. 173-211.
26. H. Lowry, N.J. Rosebrough, L.A. Farr, R.J. Randal, J. Biol. Chem. 193 (1951) 265.
27. G. Cooper, The Tools of Biochemistry, Wiley-Interscience, New York, 1977, pp. 398-399.
28. G. Righetti, in T.S. Work, R.H. Burda (Eds.), Isoelectric Focusing; Theory, Methodology and Application, Elsevier Biomedical Press, Amsterdam, 1987, p. 290.
29. D. Tsuru, J.D. McConn, K.T. Yasunobu, J. Biol. Chem. 240 (6) (1965) 2415-2420.