Gating strategy for immunophenotyping of leukemia and lymphoma

American Journal of Clinical Pathology

Volumn 108, Issue 2, 1997, Pages 152-157

  Sun, Tsieh ^a,b   Sangaline, Rita ^a   Ryder, John ^a,b   Gibbens, Karen ^a   Rollo, Christina ^a   Stewart, Suzanne ^a   Rajagopalan, Chitra ^a,b  

^a VETERANS AFFAIRS MEDICAL CENTER   (United States)

^b UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

Author keywords

Gating strategy; Immunophenotyping; Leukemia; Lymphoma; Right angle light scatter CD45 gating combination

Indexed keywords

ARTICLE; CELL ISOLATION; FLOW CYTOMETRY; HUMAN; HUMAN CELL; IMMUNOPHENOTYPING; LEUKEMIA; LIGHT SCATTERING; LYMPHOMA; PRIORITY JOURNAL; TUMOR CELL;

EID: 0030837882     PISSN: 00029173     EISSN: None     Source Type: Journal    
DOI: 10.1093/ajcp/108.2.152     Document Type: Article

References (13)

1. Festin R, Bjorkland A, Totterman TH. Multicolor flow cytometric analysis of the CD45 antigen provides improved lymphoid cell discrimination in bone marrow and tissue biopsies. J Immunol Methods. 1994;177:215-224.
2. Stelzer GT, Shults KE, Loken MR. CD45 gating for routine flow cytometric analysis of human bone marrow specimens. Ann N Y Acad Sci. 1993;677:265-280.
3. Loken MR, Brosnan J, Bach BA, Ault KA. Establishing optimal lymphocyte gates for immunophenotyping. Cytometry. 1990;11:453-459.
4. Borowitz MJ, Guenther KL, Shults KE, Stelzer GT. Immunophenotyping of acute leukemia by flow cytometric analysis: use of CD45 and right-angle light scatter to gate on leukemic blasts in three-color analysis. Am J Clin Pathol. 1993;100:534-540.
5. Rainer RO, Hodges L, Stelzer GT. CD45 gating correlates with bone marrow differential. Cytometry. 1995;22:139-145.
6. Nicholson JKA, Jones BM, Hubbard M. CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993;14:685-689.
7. Beverly PCL, Lynch D, Delia D. Isolation of human hematopoietic progenitor cells using monoclonal antibodies. Nature. 1980;287:332-333.
8. Shah VO, Civin CI, Loken MR. Flow cytometric analysis of human bone marrow, IV. Differential quantitative expression of T-200 common leukocyte antigen during normal hematopoiesis. J Immunol. 1988;140:1861-1867.
9. Terstappen LWMM, Konemann S, Safford M, et al. Flow cytometric characterization of acute myeloid leukemia, part 1: significance of light scattering properties. Leukemia. 1991;5:315-321.
10. Braylan R. Lymphomas. In: Bauer KD, Duque RE, Shankey TV, eds. Clinical Flow Cytometry. Baltimore, Md: Williams & Wilkins; 1993:203-235.
11. Terstappen LWMM, Johnsen S, Segers-Nolten IMJ, Loken MR. Identification and characterization of plasma cells in normal human bone marrow by high-resolution flow cytometry. Blood. 1990;76:1739-1747.
12. Caldwell CW, Peterson WP. Relationship between CD45 antigen expression and putative stages of differentiation in B-cell malignancies. Am J Hematol. 1991;36:111-115.
13. Peters RE, Janossy G, Ivory K, Al-Ismail S, Mercolino T. Leukemia associated changes identified by quantitative flow cytometry, III. B-cell gating in CD37/kappa/lambda clonality test. Leukemia. 1994;8:1864-1870.