Intronic sequences modulate the sensitivity of β-lactoglobulin transgenes to position effects

Gene

Volumn 193, Issue 2, 1997, Pages 239-243

  Webster, John ^a   Donofrio, Gaetano ^b   Wallace, Roberta ^a   Clark, A John ^a   Whitelaw, C Bruce A ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF PARMA   (Italy)

Author keywords

Gene silencing; Intron; Mammary; Ovine

Indexed keywords

BETA LACTOGLOBULIN;

ANIMAL CELL; ARTICLE; GENE DELETION; GENE EXPRESSION; GENE NUMBER; GENE STRUCTURE; INTRON; MAMMARY GLAND; MOUSE; NONHUMAN; PRIORITY JOURNAL; TRANSGENE;

ANIMALS; INTRONS; LACTOGLOBULINS; MICE; MICE, TRANSGENIC; TRANSGENES;

ANIMALIA; OVIS;

EID: 0030837497     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00128-5     Document Type: Article

References (19)

1. Archibald, A.L., McClenaghan, M., Hornsey, V., Simons, J.P., Clark, A.J., 1990. High-level expression of biologically active human α1-antitrypsin in the milk of transgenic mice. Proc. Natl. Acad. Sci. USA 87, 5178-5182.
2. Barash, I., Nathan, M., Kari, R., Ilan, N., Shani, M., Hurwitz, D.R., 1996. Elements within the β-lactoglobulin gene inhibit expression of human serum albumin cDNA and minigenes in transfected cells but rescue their expression in the mammary gland of transgenic mice. Nucleic Acids Res. 24, 602-610.
3. Beckmann, J.S., Trifonov, E.N., 1991. Splice junctions follow 205-base ladder. Proc. Natl. Acad. Sci. USA 88, 2380-2383.
4. Bonifer, C., Huber, M.C., Bosch, F.Z., Sippel, A.E., 1994. Dissection of the locus control function located on the chicken lysozyme gene domain in transgenic mice. Nucleic Acids Res. 22, 4202-4210.
5. Brinster, R.L., Allen, J.M., Behringer, R.R., Gelinas, R.E., Palmiter, R.D., 1988. Introns increase transcriptional efficiency in transgenic mice. Proc. Natl. Acad. Sci. USA 85, 836-840.
6. Burdon, T.G., Maitland, K.A., Clark, A.J., Wallace, R.M., Watson, C.J., 1994. Regulation of the sheep β-lactoglobulin gene by lactogenic hormones is mediated by a transcription factor that binds an interferon-γ activation site-related element. Mol. Endocrinol. 8, 1528-1536.
7. Farini, E., Whitelaw, C.B.A., 1995. Ectopic expression of β-lactoglobulin transgenes. Mol. Gen. Genet. 246, 734-738.
8. Festenstein, R., Tolaini, M., Corbella, P., Mamalaki, C., Parrington, J., Fox, M., Miliou, A., Jones, M., Kioussis, D., 1996. Locus control region function and heterochromatin-induced position effect variegation. Science 271, 1123-1125.
9. Grosveld, F., van Assendelft, G.B., Greeves, D.R., Kollias, G., 1987. Position independent, high level expression of the human beta-globin gene. Cell 51, 975-985.
10. Kellum, R., Schedl, P., 1992. A group of ses elements function as domain boundaries in an enhancer-blocking assay. Mol. Cell. Biol. 12, 2424-2431.
11. McKnight, R.A., Spencer, M., Wall, R.J., Hennighausen, L., 1992. Severe position effects imposed on a 1kb mouse whey acidic protein gene promoter are overcome by heterologous matrix attachment regions. Mol. Reprod. Dev. 44, 179-184.
12. Millonig, J.H., Emerson, J.A., Levorse, J.M., Tilghman, S.M., 1995. Molecular analysis of the distal enhancer of the mouse α-fetoprotein gene. Mol. Cell. Biol. 15, 3848-3856.
13. O'Donnell, K., Chen, C., Wensink, P., 1994. Insulating DNA directs ubiquitous transcription of the Drosophila melanogaster αI-tubulin gene. Mol. Cell. Biol. 14, 6398-6408.
14. Ptashne, M., 1986. Gene regulation by proteins acting nearby and at a distance. Nature 322, 697-701.
15. Shani, M., Barash, I., Nathamn, M., Ricca, G., Searfoss, G.H., Dekel, I., Faerman, A., Givol, D., Hurwitz, D.R., 1992. Expression of human serum albumin in the milk of transgenic mice. Transg. Res. 1, 195-208.
16. Tsukiyama, T., Becker, P.B., Wu, C., 1994. ATP dependent nucleosome disruption at a heat shock promoter mediated by binding of GAGA transcription factor. Nature 367, 525-532.
17. Webster, J., Wallace, R.M., Clark, A.J., Whitelaw, C.B.A., 1995. Tissue-specific, temporally regulated expression mediated by the proximal ovine β-lactoglobulin promoter in transgenic mice. Cell. Mol. Biol. Res. 41, 11-16.
18. Whitelaw, C.B.A., Archibald, A.L., Harris, S., McClenaghan, M., Simons, J.P., Clark, A.J., 1991. Targeting expression to the mammary gland: intronic sequences can enhance the efficiency of gene expression in transgenic mice. Transg. Res. 1, 3-13.
19. Whitelaw, C.B.A., Harris, S., McClenaghan, M., Simons, J.P., Clark, A.J., 1992. Position-independent expression of the ovine β-lactoglobulin gene in transgenic mice. Biochem. J. 286, 31-39.