Ultrafast cleavage and deprotection of oligonucleotides synthesis and use of C(Ac) derivatives

Nucleosides and Nucleotides

Volumn 16, Issue 7-9, 1997, Pages 1589-1598

  Reddy, M P ^a   Hanna, Naeem B ^a   Farooqui, Firdous ^a  

^a BECKMAN COULTER INC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE;

CONFERENCE PAPER; DNA SYNTHESIS; DRUG SYNTHESIS; NUCLEOTIDE METABOLISM; TRANSAMINATION;

EID: 0030832994     PISSN: 07328311     EISSN: None     Source Type: Journal    
DOI: 10.1080/07328319708006236     Document Type: Conference Paper

References (24)

1. Reddy, M. P., Hanna, N. B. and Farooqui, F. (1994) Tetrahedron Lett., 35, 4311-4314.
2. It is available from Beckman Instruments and Glen Research under the name "UltraFAST Cleavage and Deprotection System".
3. Reddy, M. P., Farooqui, F. and Hanna, N. B. (1995) Tetrahedron Lett., 36, 8929-8932.
4. Ammonium hydroxide was used as one of the components to increase the solubiliy of DNA, especially, when a nonaqueous reagent such as propylamine was used.
5. The reactivity of methylamine/ammonium hydroxide was very close to that of methylamine itself. However, we have chosen methylamine/ammonium hydroxide since this reagent was more compatible with certain polymer materials used in packaging.
6. Weber, H. and Khorana, H. G. (1972) J. Mol. Biol., 72, 219-249.
7. Miller, P. S., Reddy, M. P., Murakami, A., Blake, K. R., Lin, S. and Agris, C. H. (1986) Biochemistry, 25, 5092-5097.
8. MAcMillan, A. M. and Verdine, G. L. (1991) Tetrahedron, 47, 2603-2616.
9. Hogrefe, R. I., Vaghefi, M. M., Reynolds, M. A., Young, K. M. and Arnold, L. J. (1993) Nucleic Acids Res., 21, 2031-2038.
10. Polushin, N. N., Morocho, A. M., Chen, B. C. and Cohen, J. S. (1994) Nucleic Acids Res., 22, 639-645.
11. Polushin, N. N., Pashkova, I. N. and Effimov, V. A. (1991) Nucleic Acids Res. Symp. Ser., No. 24, 49-50.
12. Kraszewski, A., Delort, A. M. and Teoule, R. (1986) Tetrahedron Lett., 27, 861-864.
13. Bhat, V., Ugarkar, B. G., Sayeed, V. A., Grimm, K., Kosora, N., Domenico, P. A. and Stocker, E. (1989) Nucleosides and Nucleotides, 8, 179-183.
14. Ti, G. S., Gaffney, B. L. and Jones, R. A. (1982) J. Am. Chem. Soc., 104, 1316-1319.
15. Sinha, N. D., Biernat, J., Mcmanus, J and Koster, H. (1984) Nucleic Acids Res., 12, 4539-4557.
16. Barone, A. D., Tang, I. Y. and Caruthers, M. H. (1984) Nucleic Acids Res., 12, 4051-4061.
17. Pon, R. T., Usman, N. and Ogilvie, K. K. (1988) Biotechniques, 6, 768-775.
18. Garegg, P.T., Rogberg, T., Stawinski, J. and Stromberg, R. (1986) Chemica Scripta, 26, 59-62.
19. Markiewicz, W. T. (1979) J. Chem. Res.(S), 24-25.
20. Inoue, H., Hayase, Y., Imusa, A., Iwai. S., Miura, K. and Ohtsuka, E. (1987) Nucleic Acids Res., 15, 1631-1648.
21. Ngilas, A. and Chattopadhyaya, J. (1986) Acta Chem. Scand., B40, 826-830.
22. Iyer, R. P., Egan, W., Regan, J. B. and Beaucage, S. L. (1990) J. Am. Chem. Soc., 112, 1253-1254.
23. Phosphoramidites are usually stored at -20°C to 4°C. However, we performed the accelerated stability at 37°C in order to obtain quick results.
24. Schulhof, J. C., Molko, D. and Teoule, R. (1987) Nucleic Acids Res., 15, 397-416.