Mannitol dehydrogenase from Rhodobacter sphaeroides Si4: Subcloning, overexpression in Escherichia coli and characterization of the recombinant enzyme

Applied Microbiology and Biotechnology

Volumn 48, Issue 1, 1997, Pages 47-52

  Schafer A ^a   Stein, M A ^a   Schneider, K H ^a   Giffhorn, F ^a  

^a SAARLAND UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

MANNITOL DEHYDROGENASE;

ARTICLE; ENZYME ANALYSIS; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETIC RECOMBINATION; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; POLYMERASE CHAIN REACTION; RHODOBACTER SPHAEROIDES; RNA TRANSLATION;

CLONING, MOLECULAR; ESCHERICHIA COLI; GENES, BACTERIAL; MANNITOL DEHYDROGENASE; RECOMBINANT PROTEINS; RHODOBACTER SPHAEROIDES;

ESCHERICHIA COLI; RHODOBACTER SPHAEROIDES; RHODOPSEUDOMONAS;

EID: 0030832513     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051013     Document Type: Article

References (34)

1. Ansorge W (1982) Fast visualization of protein hands by impregnation in potassium permanganate and silver nitrate. Electrophoresis 82: 235-242
2. Armengaud J, Jouanneau Y (1995) Overexpression in Escherichia coli of the fdxA gene encoding Rhodobacter capsulatus ferredoxin II. Protein Expr Purif 6: 176-184
3. Armengaud J, Meyer C, Jouanneau Y (1994) Recombinant expression of the fdxD gene of Rhodobacter capsulatus and characterization of its product, a [2Fe - 2S] ferredoxin. Biochem J 300: 413-418
4. Aukhil I, Joshi P, Yan Y, Erickson HP (1993) Cell and heparin-binding domains of the hexabrachion arm identified by tenascin expression proteins. J Biol Chem 268: 2542-2553
5. Blackwell JR, Morgan R (1991) A novel strategy for production of a highly expressed recombinant protein in an active form. FEBS Lett 295: 10-12
6. Bollivar DW, Jiang Z-Y, Bauer CE, Beale SI (1994) Heterologous expression of the bchM gene product from Rhodobacter capsulatus and demonstration that it encodes S-adenosyl-L-methionine:Mg-protoporphyrin IX methyltransferase. J Bacteriol 176: 5290-5296
7. Cardamone M, Puri NK, Brandon MR (1995) Comparing the refolding and reoxidation of recombinant porcine growth hormone from a urea denatured state and from Escherichia coli inclusion bodies. Biochemistry 34: 5773-5794
8. Cote RJ, Gherna RL (1994) Nutrition and media. In: Gerhardt P, Murray RGE, Wood WA, Krieg NA (ed) Methods for general and molecular bacteriology. American Society for Microbiology, Washington, DC, pp 155-178
9. Darby NJ, Creighton TE (1990) Folding proteins. Nature 344: 715-716
10. Das A (1990) Overproduction of proteins in Escherichia coli: vectors, hosts and strategies. Methods Enzymol 182: 93-112
11. Georgiou G, Valax P, Ostermeier M, Horowitz PM (1994) Folding and aggregation of TEM β-lactamase: analogies with the formation of inclusion bodies in Escherichia coli. Protein Sci 3:1953-1960
12. Gold L (1988) Posttranscriptional regulatory mechanisms in Escherichia coli. Annu Rev Biochem 57: 199-233
13. Hanahan D (1983) Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166: 557-580
14. Hartley DL, Kane JF (1988) Properties of inclusion bodies from recombinant Escherichia coli. Biochem Soc Trans 16: 101-102
15. Hoess A, Arthur AK, Wanner G, Fanning E (1988) Recovery of soluble, biologically active recombinant proteins from total bacterial lysates using ion exchange resin. Biotechnology 6: 1214-1217
16. Hoffman BJ, Broadwater JA, Johnson P, Harper J, Fox BG, Kenealy WR (1995) Lactose fed-batch overexpression of recombinant metalloproteins in Escherichia coli BL21(DE3): process control yielding high levels of metal-incorporated, soluble protein. Protein Expr Purif 6: 646-654
17. Kohno T, Carmichael DK, Sommer A, Thompson RC (1990) Refolding of recombinant proteins. Methods Enzymol 185: 187-195
18. 1-antitrypsin expressed as inclusion bodies in Escherichia coli: Characterization of aggregation. Biochim Biophys Acta 1247: 179-184
19. Lang HP, Cogdell RJ, Gardiner AT, Hunter CN (1994) Early steps in carotenoid biosynthesis: sequences and transcriptional analysis of the ctrI and ctrB genes of Rhodobacter sphaeroides and overexpression and reactivation of ctrI in Escherichia coli and R. sphaeroides. J Bacteriol 176: 3859-3869
20. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 227: 680-685
21. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265-275
22. Marston FA (1986) The purification of eukaryotic polypepties synthesized in Escherichia coli. Biochem J 240: 1-12
23. Rode H, Giffhorn F (1982) D-(-)-Tartrate dehydratase of Rhodopseudomonas sphaeroides: purification, characterization, and application to enzymatic determination of D-(-)-tartrate. J Bacteriol 150: 1061-1068
24. Rosenberg AH, Lade BN, Chui D-S, Lin S-W, Dunn JJ, Studier FW (1987) Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56: 125-135
25. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
26. Scheper T, Brandes W, Grau C, Hundeck HG, Reinhardt B, Rüther F, Plötz F, Schelp P, Schügerl K, Schneider KH, Giffhorn F, Rehr B, Sahm H (1991) Application of biosensor systems for bioprocess monitoring. Anal Chim Acta 249: 25-34
27. Schneider KH, Giffhorn F (1989) Purification and properties of a polyol dehydrogenase from the phototrophic bacterium Rhodobacter sphaeroides. Eur J Biochem 184: 15-19
28. Schneider KH, Giffhorn F (1994) Overproduction of mannitol dehydrogenase in Rhodohacter sphaeroides. Appl Microbiol Biotechnol 41: 578-583
29. Schneider KH, Giffhorn F, Kaplan S (1993) Cloning, nucleotide sequence and characterization of the mannitol dehydrogenase gene from Rhodobacter sphaeroides. J Gen Microbiol 139: 2475-2484
30. Schneider KH, Jäkel G, Hoffmann R, Giffhorn F (1995) Enzyme evolution in Rhodohacter sphaeroides: selection of a mutant expressing a new galactitol dehydrogenase and biochemical characterization of the enzyme. Microbiology 141: 1865-1873
31. Schwartz D, Stein M, Schneider KH, Giffhorn F (1994) Synthesis of D-xylulose from D-arabitol by enzymatic conversion with immobilized mannitol dehydrogenase from Rhodobacter sphaeroides. J Biotechnol 33: 95-101
32. Studier FW, Rosenberg AH, Dunn JJ, Duhendorff JW (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185: 60-89
33. Suttnar J, Dyr JE, Hamsikova E, Novak J, Vonka V (1994) Procedure for refolding and purification of recombinant proteins from Escherichia coli inclusion bodies using a strong anion exchanger. J Chromatogr B Biomed Appl 656: 123-126
34. Tsai AM, Betenbaugh MJ, Shiloach J ( 1995) The kinetics of RCC1 inclusion body formation in Escherichia coli. Biotechnol Bioeng 48: 715-718