Direct PCR using tomato pollen grain suspensions

BioTechniques

Volumn 23, Issue 6, 1997, Pages 986-990

  Levin, Ilan ^a   Gilboa, Nehama ^a  

^a VOLCANI CENTER   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

AGRICULTURE; ARTICLE; DNA DETERMINATION; GENE AMPLIFICATION; GRAIN; NONHUMAN; PHENOTYPE; POLLEN; POLYMERASE CHAIN REACTION; TOMATO;

LYCOPERSICON ESCULENTUM;

EID: 0030831673     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97236bm03     Document Type: Article

References (9)

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2. Edwards, K., C. Johnstone and C. Thompson. 1991. A simple and rapid method for the preparation of genomic plant DNA for PCR analysis. Nucleic Acids Res. 19:1349.
3. Fulton, T.M., J. Chunwongse and S.D. Tanksley. 1995. Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol. Biol. Rep. 13:207-209.
4. Lin, J.-Z. and K. Ritland. 1995. Flower petals allow simpler and better isolation of DNA for plant RAPD analyses. Plant Mol. Biol. Rep. 13:210-213.
5. Saiki, R.K., S. Scharf, F. Faloona, K.B. Mullis, G.T. Horn, H.A. Erlich and N. Arnheim. 1985. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230:1350-1354.
6. Stewart, C.N. Jr. and L.E. Via. 1993. A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques 14:748-751.
7. Stub, J.E., F.C. Serguen and M. Gupata. 1996. Genetic markers, map construction, and their application in plant breeding. HortScience 31:729-741.
8. Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalsky and S.V. Tingey. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18:6531-6535.
9. Williamson, V.M., J.-Y. Ho, F.F. Wu, N. Miller and I. Kaloshian. 1994. A PCR-based marker tightly linked to the nematode resistance gene, Mi, in tomato. Theor. Appl. Genet. 87:757-763.