Differential screening of a subtracted cDNA library: A method to search for genes preferentially expressed in multiple tissues

BioTechniques

Volumn 23, Issue 6, 1997, Pages 1084-1086

  Jin, H ^a   Cheng, X ^a   Diatchenko, L ^a   Siebert, P D ^a   Huang, C C ^a  

^a CLONTECH Laboratories Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA;

ANIMAL CELL; ARTICLE; DNA DETERMINATION; DNA LIBRARY; FEMALE; GENE EXPRESSION REGULATION; GENE INSERTION; GENETIC SCREENING; HYBRIDIZATION; IMAGE SUBTRACTION; MALE; MOLECULAR CLONING; NONHUMAN;

ANIMALIA;

EID: 0030831641     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97236st05     Document Type: Article

References (6)

1. Akopian, A.N. and J.N. Wood. 1995. Peripheral nervous system-specific genes identified by subtractive cDNA cloning. J. Biol. Chem. 270:21264-21270.
2. Diatchenko, L., Y.C. Lau, A.P. Campbell, A. Chenchik, F. Moqadam, B. Huang, S. Lukyanov, K. Lukyanov et al. 1996. Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries. Proc. Natl. Acad. Sci. USA 93:6025-6030.
3. Gurskaya, N.G., L. Diatchenko, A. Chenchik, P.D. Siebert, G.L. Khaspekov, K.A. Ludyanpv, L.L. Vagner, O.D. Ermolaeva et al. 1996. Equalizing cDNA subtraction based on selective suppression of polymerase chain reaction: cloning of Jurkat cell transcripts induced by phytohemaglutinin and phorbol 12-myristaate 13-acetate. Anal. Biochem. 240:90-97.
4. Hogue, D.L., M.J. Ellison, J.D. Yong and C.E. Cass. 1996. Identification of a novel membrane transporter associated with intracellular membranes by phenotypic complementation in the yeast saccharomyces cerevisiae. J. Biol. Chem. 271:9801-9808.
5. Sugawara, T., J.A. Holt, D. Driscoll, J.F. Strauss, III, D. Lin, W.L. Miller, D. Patterson, K.P. Clancy et al. 1995. Human steroidogenic acute regulatory protein: functional activity in Cos-1 cells, tissue-specific expression, and mapping of the structural gene to 8p11.2 and a pseudogene to chromosome 13. Proc. Natl. Acad. Sci. USA 92:4778-4782.
6. Thibodequ, J., G. Gradwohl, C. Dumas, S. Clairoux-Moreau, G. Brunet, C. Penning, G.G. Poirier and P. Morcau. 1989. Cloning of rodent cDNA encoding the poly(ADP-ri- bose) polymerase catalytic domain and analysis of mRNA levels during the cell cycle. Biochem. Cell. Biol. 67:653-660.