Immunolocalization of the pseudorabies virus immediate-early protein IE180 by immunoperoxidase staining

Journal of Virological Methods

Volumn 66, Issue 2, 1997, Pages 219-226

  Huang, Chienjin ^a   Lin, Ying Shiow ^a   Cheng, Jing Wen ^a   Chang, Tien Jye ^a  

^a NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Immediate early protein; Immunoperoxidase staining; Pseudorabies virus

Indexed keywords

ANIMAL CELL; ANTIBODY SPECIFICITY; ARTICLE; GENE EXPRESSION REGULATION; IMMEDIATE EARLY GENE; IMMUNOPEROXIDASE STAINING; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; PSEUDORABIES HERPETOVIRUS; RECOMBINANT DNA TECHNOLOGY; SWINE; TRANSACTIVATION; VIRUS DETECTION; VIRUS GENOME;

ANIMALS; ANTIBODIES, VIRAL; CATTLE; CELL LINE; CELL NUCLEUS; CYTOPLASM; ESCHERICHIA COLI; HERPESVIRUS 1, SUID; IMMEDIATE-EARLY PROTEINS; IMMUNOENZYME TECHNIQUES; KIDNEY; MICE; MICE, INBRED BALB C; MOLECULAR WEIGHT; RECOMBINANT FUSION PROTEINS;

ANIMALIA; HERPETOVIRUS; SUID HERPESVIRUS 1; SUS SCROFA;

EID: 0030829304     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0166-0934(97)00069-4     Document Type: Article

References (16)

1. Ben-Porat, T., Kervina, M., Kaplan, A.S., 1975. Early functions of the genome of herpesrirus. Virology 65, 355-362.
2. Cheung, A.K., 1989. DNA nucleotide sequence analysis of the immediate-early gene of pseudorabies virus. J. Virol. 63, 2908-2913.
3. Cheung, A.K., Vlcek, C., Paces, V., Schwyser, M., 1990. Update and comparison of the immediate-early gene DNA sequences of two pseudorabies virus isolates. Virus Genes 4, 261-265.
4. Chlan, C.A., Coulter, C., Feldman, L.T., 1987. Binding of the pseudorabies virus immediate-early protein to single-stranded DNA. J. Virol. 61, 1855-1860.
5. Feldman, L.T., Rixon, F.J., Jean, J-H., Ben-Porat, T., Kaplan, A.S., 1979. Transcription of the genome of pseudorabies virus (a herpesvirus) is strictly controlled. Virology 97, 316-327.
6. Feldman, L.T., Demarchi, J.M., Ben-Porat, T., Kaplan, A.S., 1982. Control of abundance of immediate-early mRNA in herpesvirus (pseudorabies)-infected cell. Virology 116, 250-263.
7. Fenwick, M.L., Clark, J., 1983. The effect of cycloheximide on the accumulation and stability of functional α-mRNA in cells infected with herpes simplex virus. J. Gen. Virol. 64, 1955-1963.
8. Green, M.R., Treisman, R., Maniatis, T., 1983. Transcriptional activation of cloned human β-globin genes by viral immediate-early gene products. Cell 35, 137-148.
9. Ihara, S., Feldman, L., Watanabe, S., Ben-Porat, T., 1983. Characterization of the immediate-early functions of pseudorabies virus. Virology 131, 437-454.
10. Laemmli, U.K., 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
11. Ono, E., Sakoda, Y., Taharaguchi, S., Watanabe, S., Tonomura, N., Kida, H., Shimisu, Y., 1995. Inhibition of pseudorabies virus replication by a chimeric trans-gene product repressing transcription of the immediate-early gene. Virology 210, 128-140.
12. Roizman, B., 1991. Herpesviridae. In: Francki, R.I.B., Fanguet, C.M., Knudson, D.L., Brown, F. (Eds.), Classification and Nomenclature of Viruses: Fifth Report of the International Committee on Toxonomy of Viruses. Springer, Wien New York, pp. 103-123.
13. Todd, D., Mcferran, J.B., 1985. Restriction endonuclease analysis of Aujeszky's diseases (pseudorabies) virus DNA: comparison of Northern Ireland isolates and isolates from other countries. Arch. Virol. 88, 167-176.
14. Towbin, H., Staehelin, T., Gordon, J., 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350-4354.
15. Wright, B.D., Scholey, J.M., 1993. Nonfluorescent immunolocalization of antigens in mitotic sea urchin blastomeres. Methods Cell Biol. 37, 223-239.
16. Yamada, S., Shimiza, M., 1994. Isolation and characterization of mutants of pseudorabies virus with deletion in the immediate-early regulatory gene. Virology 199, 366-375.