Characterization of two extracellular proteinases from Aspergillus terreus and their role in the formation of low molecular weight endoglucanases

Process Biochemistry

Volumn 32, Issue 8, 1997, Pages 685-689

  Stefanova, Miglena E ^a   Beletskaya, Olga P ^b   Kulaev, Igor S ^b  

^a INSTITUTE OF MICROBIOLOGY   (Bulgaria)

^b SKRYABIN INSTITUTE OF BIOCHEMISTRY AND PHYSIOLOGY OF MICROORGANISMS   (Russian Federation)

Author keywords

Aspergillus terreus; Cellulase; Cellulase multiplicity; Limited proteolysis; Proteinase; Purification

Indexed keywords

GLUCAN SYNTHASE; METALLOPROTEINASE; PROTEINASE;

ARTICLE; ASPERGILLUS TERREUS; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME SYNTHESIS; NONHUMAN; PH;

ASPERGILLUS TERREUS; FUNGI;

EID: 0030829240     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0032-9592(97)00028-9     Document Type: Article

References (19)

1. 1. Tomme, P., van Tilbeurgh, H., Pettersson, G., van Damme, J., Vandekerckhove, J., Knowles, J., Teeri, T. and Claeyssens, M., Studies on the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function of two cellobiohydrolases by limited proteolysis. European Journal of Biochemistry, 1988, 170, 575-581.
2. 2. Gilkes, N. R., Kilburn, D. G., Miller, R. C. Jr. and Warren, R. A. J., Structural and functional analysis of a bacterial cellulase by proteolysis. Journal of Biological Chemistry, 1989, 264, 17802-17808.
3. 3. Abe, J., Nakajima, K. and Hizukuri, S., Alteration of the properties of Aspergillus sp. K-27 glucoamylase on limited proteolysis with subtilisin. Carbohydrate Research, 1990, 203, 129-138.
4. 4. Kim, C.-H., Kwon, S.-T., Taniguchi, H. and Lee, D.-S., Proteolytic modification of raw-starch-digesting amylase from Bacillus circulans F-2 with subtilisin: separation of the substrate-hydrolytic domain and the raw substrate-adsorbable domain. Biochimica et Biophysica Acta, 1992, 1122, 243-250.
5. 5. Haab, D., Hagspiel, K., Szakmary, K. and Kubicek, C. P., Formation of the extracellular proteases from Trichoderma reesei QM 9414 involved in cellulase degradation. Journal of Biotechnology, 1990, 16, 187-198.
6. 6. Moormann, M., Schlochtermeier, A. and Schrempt, H., Biochemical characterization of a protease involved in the processing of a Streptomyces reticuli cellulase (avicelase). Applied and Environmental Microbiology, 1993, 59, 1573-1578.
7. 7. Beletskaya, O. P., Okunev, O. N. and Kulaev, I. S., Function and regulation of cellulase biosynthesis and secretion in Aspergilluls terreus. In Problems of Biochemistry and Physiology of Microorganisms, ed. G. K. Skryabin. Pushchino, Russia, 1985, pp. 61-71.
8. 8. Stefanova, M. E., Purification of two proteinases from Aspergillus terreus by affinity chromatography. Journal of Chromatography, 1993, 639, 346-351.
9. 9. Stefanova, M., Beletskaya, O. P. and Kulaev, I. S., Extracellular proteinase spectrum of Aspergillus terreus grown on various substrates. Folia Microbiologica 1994, 39, 231-234.
10. 10. Ivanova, G. S., Beletskaya, O. P., Okunev, O. N., Golovlev, E. L. and Kulaev, I. S., Fractionation of the cellulase complex of the fungus Aspergilluls terreus. Prikladnaya Biokhimiya i Mikrobiologiya, 1983, 19, 362-368.
11. 11. Klyosov, A. A., Rabinovich, M. L., Sinitsyn, A. P., Churilova, I. V. and Grigorash, S. Y., Enzymatic hydrolysis of cellulose. I. Activity and composition of cellulase complexes from various sources. Bioorganischeskaya Khimiya, 1980, 6, 1225-1242.
12. 12. Moore, S. and Stein, W. H., Chromatographic determination of amino acids by the use of automatic recording equipment. Methods in Enzymology 1963, 6, 819-831.
13. 13. Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F., Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 1956, 28, 350-356.
14. 14. Paberit, N. Y., Pank, M. S., Lijders, M. A. and Vanatalu, K. P., Purification and properties of neutral metalloproteinase from a thermophilic bacterium Bacillus brevis. Biokhimiya, 1984, 49, 275-283.
15. 15. Yan, T.-R., Azuma, N., Kaminogawa, S. and Yamauchi, K., Purification and characterization of a substrate-size-recognizing metalloendopeptidase from Streptococcus cremoris H61. Applied and Environmental Microbiology 1987, 53, 2296-2302.
16. 16. Eriksson, K.-E. and Pettersson, B., Purification and partial characterization of two acidic proteases from the white-rot fungus Sporotrichum pulverulentum. European Journal of Biochemistry, 1982, 124, 635-642.
17. 17. Chen, H. C. and Grethlein, H. E., Effect of cellulase size reduction on activity and accessibility. Biotechnology Letters, 1988, 10, 913-918.
18. 18. Hayashida, S. and Mo, K., Production and characteristics of avicel-disintegrating endoglucanase from a protease-negative Humicola grisea var. thermoidea mutant. Applied and Environmental Microbiology, 1986, 51, 1041-1046.
19. 19. Hayashida, S. and Teramoto, Y., Production and characteristics of raw starch-digesting α-amylase from a protease-negative Aspergillus ficum mutant. Applied and Environmental Microbiology, 1986, 52, 1068-1073.