Identification and characterization of glycosylation sites in human serum clusterin

Protein Science

Volumn 6, Issue 10, 1997, Pages 2120-2133

  Kapron, James T ^a,c   Hilliard, George M ^b   Lakins, Johnathon N ^a   Tenniswood, Martin P R ^a   West, Karen A ^a   Carr, Steven A ^b   Crabb, John W ^a  

^a Adirondack Biomedical Research Institute Inc   (United States)

^b GLAXOSMITHKLINE   (United Kingdom)

^c Adv BioAnalytical Services Inc   (United States)

Author keywords

Amino acid sequencing; Carbohydrate; Clusterin; Glycoprotein; Glycosylation; High performance liquid chromatography; Mass spectrometry; N linked oligosaccharides

Indexed keywords

CLUSTERIN;

AMINO ACID SEQUENCE; ARTICLE; BLOOD LEVEL; CELL AGGREGATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MASS SPECTROMETRY; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN GLYCOSYLATION;

EID: 0030829027     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560061007     Document Type: Article

References (26)

1. Bean MF, Annan RS, Hemling ME, Mentzer M, Huddleston MJ, Carr SA. 1995. LC-MS methods for selective detection of posttranslational modifications in proteins: Glycosylation, phosphorylation, sulfation and acylation. In: Crabb JW, ed. Techniques in protein chemistry VI. San Diego: Academic Press. pp 107-116.
2. Blaschuk O, Burdzy K, Fritz I. 1983. Purification and characterization of a cell-aggregating factor (clusterin), the major glycoprotein in ram rete testis fluid. J Biol Chem 12:7714-7720.
3. Blaschuk O, Fritz I. 1984. Isoelectric forms of clusterin isolated from ram rete testis fluid and from secretions of primary cultures of ram and rat Sertoli-cell-enriched preparations. Can J Biochem Cell Biol 62:456-461.
4. Burkey B, de Silva H, Harmony J. 1991. Intracellular processing of apolipoprotein J precursor to the mature heterodimer. J Lipid Res 32:1039-1048.
5. Carr S, Huddleston M, Bean M. 1993. Selective identification and differentiation of N-and O-linked oligosaccharides in glycoproteins by liquid chromatography-mass spectrometry. Protein Sci 2:183-196.
6. Choi-Miura NH, Takahashi Y, Nakano Y, Tobe T, Tomita M. 1992. Identification of the disulfide bonds in human plasma protein SP-40,40 (apolipoprotein-J). J Biochem 112:557-561.
7. Crabb JW, Johnson CM, Carr SA, Armes LG, Saari JC. 1988. The complete primary structure of the cellular retinaldehyde-binding protein from bovine retina. J Biol Chem 263:18678-18687.
8. de Silva HV, Harmony JAK, Stuart WD, Gil CM, Robbins J. 1990a. Apolipoprotein J: Structure and tissue distribution. Biochemistry 29:5380-5389.
9. de Silva HV, Stuart WD, Park YB, Mao SJT, Gil CM, Wetterau JR, Busch SJ, Harmony JAK. 1990b. Purification and characterization of apolipoprotein J. J Biol Chem 265:14292-14297.
10. Griswold M, Roberts K, Bishop P. 1986. Purification and characterization of a sulfated glycoprotein secreted by Sertoli cells. Biochemistry 25:7265-7270.
11. Hansen JE, Lund O, Engelbrecht J, Bohr H, Nielsen JO, Hansen JES, Brunak S. 1995. Prediction of O-glycosylation of mammalian proteins: Specificity patterns of UDP-galnac:polypeptide N-acetylgalaclosaminyltransferase. Biochem J 308:801-813.
12. Hardardottir I, Kunitake S, Moser A, Doerrler W, Rapp J, Grunfeld C, Feingold K. 1994. Endotoxin and cytokines increase hepatic messenger RNA levels and serum concentrations of apolipoprotein J (clusterin) in Syrian hamsters. J Clin Invest 94:1304-1309.
13. Huddleston MJ, Bean MF, Carr SA. 1993. Collisional fragmentation of glycopeptides by electrospray ionization LC/MS and LC/MS/MS: Methods for selective detection of glycopeptides in protein digests. Anal Chem 65:877-884.
14. Jenne DE, Tschopp J. 1989. Molecular structure and functional characterization of a human complement cytolysis inhibitor found in blood and seminal plasma: Identity to sulfated glycoprotein 2, a constituent of rat testis fluid. Proc Natl Acad Sci USA 86:7123-7127.
15. Kirszbaum L, Bozas SE, Walker ID. 1992. SP-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges. FEBS Lett 297:70-76.
16. Kirszbaum L, Sharpe JA, Murphy B, d'Apice AJF, Classon B, Hudson P, Walker ID. 1989. Molecular cloning and characterization of the novel, human complement-associated protein, SP-40,40: A link between the complement and reproductive systems. EMBO J 8:711-718.
17. Roberts GD, Johnson WP, Burman S, Anumula KR, Carr SA. 1995. An integrated strategy for structural characterization of monoclonal antibodies: Application to anti-respiratory syncytial virus Mab. Anal Chem 67:3613-3625.
18. Rosenberg M, Silkensen J. 1995. Clusterin: Physiologic and pathophysiologic considerations. Int J Biochem Cell Biol 27:633-645.
19. Stone KL, Elliott JI, Peterson G, McMurray W, Williams KR. 1990. Reversed-phase high performance liquid chromatography for fractionation of enzymatic digests and chemical cleavage products of proteins. Methods Enzymol 193:389-412.
20. Sylvester S, Skinner M, Griswold M. 1984. A sulfated glycoprotein synthesized by Sertoli cells and by epididymal cells is a component of the sperm membrane. Biol Reprod 31:1087-1101.
21. Wagner M, Morgans C, Koch-Brandt C. 1995. The oligosaccharides have an essential but indirect role in sorting GP80 (clusterin, TRPM-2) to the apical surface of MDCK cells. Eur J Cell Biol 67:84-88.
22. West KA, Crabb JW. 1992. Applications of automatic PTC amino acid analysis. In: Angeletti R, ed. Techniques in protein chemistry III. San Diego: Academic Press. pp 233-242.
23. Wilson M, Easterbrook-Smith S. 1992. Clusterin binds by a multivalent mechanism to the Fc and Fab regions of IgG. Biochim Biophys Acta 1159:319-326.
24. Wilson M, Easterbrook-Smith S, Taillefer D, Lakins J, Tenniswood M. 1995. The role of clusterin at sites of active cell death. In: Harmony J, ed. Clusterin: Role in vertebrate development function and adaption. Material Molecular Biology Intelligence Unit. Austin, Texas: RG Landes Co. pp 95-112.
25. Wong P, Pineault J, Lakins J, Taillefer D, Léger J, Wang C, Tenniswood M. 1993. Genomic organization and expression of the rat TRPM-2 (clusterin) gene, a gene implicated in apoptosis. J Biol Chem 268:5021-5031.
26. Wong P, Taillefer D, Lakins J, Pineault J, Chader G, Tenniswood M. 1994. Molecular characterization of human TRPM-2/clusterin, a gene associated with sperm maturation, apoptosis and neurodegeneration. Eur J Biochem 221:917-925.