Human fatty acid synthase: Assembling recombinant halves of the fatty acid synthase subunit protein reconstitutes enzyme activity

Proceedings of the National Academy of Sciences of the United States of America

Volumn 94, Issue 23, 1997, Pages 12326-12330

  Jayakumar, Arumugam ^a   Chirala, Subrahmanyam S ^a   Wakil, Salih J ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Active site recognition; Subunit organization

Indexed keywords

ACETYL COENZYME A; FATTY ACID SYNTHASE; HYBRID PROTEIN; MALONYL COENZYME A; MYRISTIC ACID; PALMITIC ACID; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; STEARIC ACID; THIOREDOXIN;

ARTICLE; ENZYME RECONSTITUTION; ESCHERICHIA COLI; FATTY ACID SYNTHESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ASSEMBLY;

ENZYME ACTIVATION; FATTY ACID SYNTHETASE COMPLEX; HUMANS; MODELS, MOLECULAR; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

ANIMALIA; ESCHERICHIA COLI;

EID: 0030827630     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.94.23.12326     Document Type: Article

References (26)

1. Jayakumar, A., Tai, M.-H., Huang, W.-Y., Al-Feel, W., Hsu, M., Abu-Elheiga, L., Chirala, S. S. & Wakil, S. J. (1995) Proc. Natl. Acad. Sci. USA 92, 8695-8699.
2. Wakil, S. J. (1989) Biochemistry 28, 4523-4530.
3. Stoops, J. K., Ross, P. R., Arslanian, M. J., Aune, K. C., Wakil, S. J. & Oliver, R. M. (1979) J. Biol. Chem. 254, 7418-7426.
4. Kitamoto, T., Nishigai, M., Ikai, A., Ohashi, K. & Seyama, Y. (1985) Biochim. Biophys. Acta 827, 164-173.
5. Stoops, J. K., Wakil, S. J., Uberbacher, E. C. & Bunick, G. J. (1987) J. Biol. Chem. 262, 10246-10251.
6. Mattick, J. S., Tsukamoto, Y., Nickless, J. & Wakil, S. J. (1983) J. Biol. Chem. 258, 15291-15299.
7. Mattick, J. S., Nickless, J., Mizugaki, M., Yang, C-Y., Uchiyama, S. & Wakil, S. J. (1983) J. Biol. Chem. 258, 15300-15304.
8. Wong, H., Mattick, J. S. & Wakil, S. J. (1983) J. Biol. Chem. 258, 15305-15311.
9. Tsukamoto, Y., Wong, H., Mattick, J. S. & Wakil, S. J. (1983) J. Biol. Chem. 258, 15312-15322.
10. Tsukamoto, Y. & Wakil, S. J. (1988) J. Biol. Chem. 263, 16225-16229.
11. Chirala, S. S., Kasturi, R., Pazirandeh, M., Stolow, D. T., Huang, W.-Y. & Wakil, S. J. (1989) J. Biol. Chem. 264, 3750-3757.
12. Amy, C. M., Witkowski, A., Naggert, J., Williams, B., Randhawa, Z. & Smith, S. (1989) Proc. Natl. Acad. Sci. USA 86, 3114-3118.
13. Kameda, K. & Goodridge, A. G. (1991) J. Biol. Chem. 266, 419-426.
14. Holzer, K. R., Liu, W. & Hammes, G. G. (1989) Proc. Natl. Acad. Sci. USA 86, 4387-4391.
15. Beck, K.-I., Schreglmann, R., Stathopulos, I., Klein, H., Hoch, J. & Schweizer, M. (1992) DNA Sequence 2, 359-386.
16. Chirala, S. S., Huang, W.-Y., Jayakumar, A., Sakai, K. & Wakil, S. J. (1997) Proc. Natl. Acad. Sci. USA 94, 5588-5593.
17. Jayakumar, A., Huang, W.-Y., Raetz, B., Chirala, S. S. & Wakil, S. J. (1996) Proc. Natl. Acad. Sci. USA 93, 14509-14514.
18. Joshi, A. K. & Smith, S. (1993) J. Biol. Chem. 268, 22508-22513.
19. Stoops, J. K. & Wakil, S. J. (1981) J. Biol. Chem. 256, 5128-5133.
20. Stoops J. K. & Wakil, S. J. (1982) J. Biol. Chem. 257, 3230-3235.
21. Stoops, J. K. & Wakil, S. J. (1982) Biochem. Biophys. Res. Commun. 104, 1018-1024.
22. Singh, N., Wakil, S. J. & Stoops, J. K. (1984) J. Biol. Chem. 259, 3605-3611.
23. Arslanian, M. J. & Wakil, S. J. (1975) Methods Enzymol. 35, 59-65.
24. Wakil, S. J., Stoops, J. K. & Joshi, V. C. (1983) Annu. Rev. Biochem. 52, 537-579.
25. Volpe, J. J. & Vagelos, P. R. (1977) Physiol. Rev. 56, 339-417.
26. Pazirandeh, M., Chirala, S. S., Huang, W.-Y. & Wakil, S. J. (1989) J. Biol. Chem. 264, 18195-18201.