Specialized Rap1p/Gcr1p transcriptional activation through Gcr1p DNA contacts requires Gcr2p, as does hyperphosphorylation of Gcr1p

Genetics

Volumn 147, Issue 2, 1997, Pages 493-505

  Zeng, Xiao ^a   Deminoff, Stephen J ^a   Santangelo, George M ^a  

^a UNIVERSITY OF SOUTHERN MISSISSIPPI   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL DNA; TRANSCRIPTION FACTOR;

ARTICLE; CONFORMATIONAL TRANSITION; FUNGUS GROWTH; GENE CONTROL; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DNA BINDING; PROTEIN PHOSPHORYLATION; SACCHAROMYCES CEREVISIAE; TRANSCRIPTION REGULATION;

DNA-BINDING PROTEINS; FUNGAL PROTEINS; GTP-BINDING PROTEINS; PHOSPHORYLATION; PROTEIN BINDING; RAP GTP-BINDING PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS;

EUKARYOTA; FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0030827114     PISSN: 00166731     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (35)

1. ADZHUBEI, A. A., and M. J. E. STERNBERG, 1993 Left-handed polyproline II helices commonly occur in globular proteins. J. Mol. Biol. 229: 472-493.
2. BITTER, G. A., K. K. H. CHANG and K. M. EGAN, 1991 A multi-component upstream activation sequence of the Saccharomyces cerevisiae glyceraldehyde-3-phosphate dehydrogenase gene promoter. Mol. Gen. Genet. 231: 22-32.
3. BOYLE, W. J., P. VAN DER GEER and T. HUNTER, 1991 Phosphopeplide mapping and phosphoamino acid analysis by two-dimensional separation on thin-layer cellulose plates, pp. 110-149 in Methods in Enzymology, Vol. 201, edited by T. HUNTER and B. M. SEFTON. Academic Press, San Diego.
4. BUCHMAN, A. R., N. F. LUE and R. D. KORNBERG, 1988 Connections between transcriptional activators, silencers and telomeres as revealed by functional analysis of a yeast DNA-binding protein. Mol. Cell. Biol. 8: 5086-5099.
5. BURNETTE, W. N., 1981 "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112: 195-203.
6. CARLSSON, S. R., P.-O. LYCKSELL and M. FUKUDA, 1993 Assignment of O-glycan attachment sites to the hinge-like regions of human lysosomal membrane glycoproteins Lamp-1 and Lamp-2. Arch. Biochem. Biophys. 304: 65-73.
7. CHAMBERS, A., E. A. PACKHAM and I. R. GRAHAM, 1995 Control of glycolytic gene expression in the budding yeast (Saccharomyces cerevisiae). Curr. Genet. 29: 1-9.
8. DEMINOFF, S. J., J. TORNOW and G. M. SANTANGELO, 1995 Unigenic evolution: a novel genetic method localizes a putative leucine zipper that mediates dimerization of the Saccharomyces cerevisiae regulator Gcr1p. Genetics 141: 1263-1274.
9. DRAZINIC, C. M., J. B. SMERAGE, M. C. LOPEZ and H. V. BAKER, 1996 Activation mechanism of the multifunctional transcription factor repressor-activator protein 1 (Rap1p). Mol. Cell. Biol. 16: 3187-3196.
10. DUMITRU, I., and J. B. MCNEIL, 1994 A simple in vivo footprinting method to examine DNA-protein interactions over the yeast PYK UAS element. Nucleic Acids Res. 22: 1450-1455.
11. FAVALORO, J., R. TRIESMAN and R. KAMEN, 1980 Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping. Methods Enzymol. 65: 718-749.
12. HENRY, Y. A. L., M. C. LOPEZ, J. M. GIBBS, A. CHAMBERS, S. M. KINGSMAN et al., 1994 The yeast protein Gcr1p binds to the PGK UAS and contributes to the activation of transcription of the PGK gene. Mol. Gen. Genet. 245: 506-511.
13. HOLLAND, M. J., T. YOKOI, J. P. HOLLAND, K. MYAMBO and M. A. INNIS, 1987 The GCR1 gene encodes a positive transcriptional regulator of the enolase and glyceraldehyde-3-phosphate dehydrogenase gene families in Saccharomyces cerevisiae. Mol. Cell. Biol. 7: 813-820.
14. HUIE, M. A., E. W. SCOTT, C. M. DRAZINTC, M. C. LOPEZ, I. K. HORNSTRA et al., 1992 Characterization of the DNA-binding activity of GCR1: in vivo evidence for two GCR1-binding sites in the upstream activating sequence of TPI of Saccharomyces cerevisiae. Mol. Cell. Biol. 12: 2690-2700.
15. JIN, S., R. K. PRUSTI, T. ROITSCH, R. G. ANKENBAUER and E. W. NESTER, 1990 Phosphorylation of the VirG protein of Agrobacterium tumefaciens by the autophosphorylated VirA protein: essential role in biological activity of VirG. J. Bacteriol. 172: 4945-4950.
16. KLEIN, C., and K. STRUHL, 1994 Protein kinase A mediates growth-regulated expression of yeast ribosomal protein genes by modulating RAP1 transcriptional activity. Mol. Cell. Biol. 14: 1920-1928.
17. LAEMMLI, K., 1970 Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227: 680-685.
18. LARKIN, J. C., J. R. THOMPSON and J. L. WOOLFORD, JR., 1987 Structure and expression of the Saccharomyces cerevisiae CRY1 gene: a highly conserved ribosomal protein gene. Mol. Cell. Biol. 7: 1764-1775.
19. PARKINSON, J. S., and E. C. KOFOID 1992 Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26: 71-112.
20. PAVLOVIC, B., and W. HÖRZ, 1988 The chromatin structure at the promoter of a glyceraldehyde phosphate dehydrogenase gene from Saccharomyces cerevisiae reflects its functional state. Mol. Cell. Biol. 8: 5513-5520.
21. SANDERS, D. A., B. L. GILLECE-CASTRO, A. M. STOCK, A. L. BURLINGAME and D. E. KOSHLAND, JR., 1989 Identification of the site of phosphorylation of the chemotaxis response regulator protein, CheY. J. Biol. Chem. 264: 21770-21778.
22. SANTANGELO, G. M., and J. TORNOW, 1990 Efficient transcription of the glycolytic gene ADH1 and three translational component genes require the GCR1 product, which can act through TUF/ GRF/RAP binding sites. Mol. Cell. Biol. 10: 859-862.
23. SCHLEIF, R., 1992 DNA looping. Annu. Rev. Biochem. 61: 199-223.
24. SCOTT, E. W., and H. V. BAKER, 1993 Concerted action of the transcriptional activators REB1, RAP1, and GCR1 in the high level expression of the glycolytic gene TPI. Mol. Cell. Biol. 13: 543-550.
25. SHORE, D., 1994 RAP1: A protean regulator in yeast. Trends Genet. 10: 408-412.
26. SHORE, D., and K. NASMYTH, 1987 Purification and cloning of a DNA binding protein from yeast that binds to both silencer and activator elements. Cell 51: 721-732.
27. RPG) found initially in genes encoding ribosomal proteins. Gene 90: 79-85.
28. TORNOW, J., and G. M. SANTANGELO, 1994 The GCR1 gene of Saccharomyces cerevisiae is a split gene with an unusually long intron. Genetics 138: 973-974.
29. TORNOW, J., X. ZENG, W. GAO and G. M. SANTANGELO, 1993 GCR1, a transcriptional activator in Saccharomyces cerevisiae, complexes with RAP1 and can function without its DNA binding domain. EMBO J. 12: 2431-2437.
30. UEMURA, H., and D. G. FRAENKEL, 1990 gcr2, a new mutation affecting glycolytic gene expression in Saccharomyces cerevisiae. Mol. Cell. Biol. 10: 6389-6396.
31. UEMURA, H., and Y. JIGAMI, 1992 Role of GCR2 in transcriptional activation of yeast glycolytic genes. Mol. Cell. Biol. 12: 3834-3842.
32. UEMURA, H., and Y. JIGAMI, 1995 Mutations in GCR1, a tianscriptional activator of Saccharomyces cerevisiae glycolytic genes, function as suppressors of gcr2 mutations. Genetics 139: 511-521.
33. WARNER, J. R., 1991 Labeling of RNA and phosphoproteins in Saccharomyces cerevisiae, pp. 423-428 in Methods in Enzymology, Vol. 194, edited by C. GUTHRIE and G. R. FINK. Academic Press, San Diego.
34. WILLIAMSON, M. P., 1994 The structure and function of proline-rich regions in proteins. Biochem. J. 297: 145-156.
35. ZHANG, J., and J. L. CORDEN, 1991 Identification of phosphorylation sites in the repetitive carboxy-terminal domain of the mouse RNA polymerase II largest subunit. J. Biol. Ghem. 266: 2290-2296.