An inexpensive chemostat apparatus for the study of microbial biofilms

Journal of Microbiological Methods

Volumn 30, Issue 2, 1997, Pages 125-132

  Whiteley, Marvin ^a   Brown, Erin ^a   McLean, Robert J C ^a  

^a TEXAS STATE UNIVERSITY   (United States)

Author keywords

Biofilm; Chemostat; Continuous culture; Modified Robbins device

Indexed keywords

APPARATUS; ARTICLE; BIOFILM; CHEMOSTAT; CONTINUOUS CULTURE; CONTROLLED STUDY; ECOSYSTEM; GENETICS; GROWTH RATE; MICROORGANISM; NONHUMAN; PHYSIOLOGY; PRIORITY JOURNAL;

EID: 0030824153     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0167-7012(97)00054-7     Document Type: Article

References (16)

1. Costerton J.W., Geesey G.G., Cheng K.-J. How bacteria stick. Sci. Am. 238:1978;86-95.
2. Davies D.G., Chakrabarty A.M., Geesey G.G. Exopolysaccharide production in biofilms: substratum activation of alginate gene expression by Pseudomonas aeruginosa. Appl. Environ. Microbiol. 59:1993;1181-1186.
3. Evans D.J., Brown M.R.W., Allison D.G., Gilbert P.D. Susceptibility of bacterial biofilms to tobramycin: role of specific growth rate and phase in division cycle. J. Antimicrob. Chemother. 25:1990;585-591.
4. Holmes C.J., Evans R.C. Resistance of bacterial biofilms to antibiotics. J. Antimicrob. Chemother. 24:1989;84.
5. Nickel J.C., Rusecka I., Wright J.B., Costerton J.W. Tobramycin resistance of Pseudomonas aeruginosa cells growing as a biofilm on urinary catheter material. Antimicrob. Agents Chemother. 27:1985;619-624.
6. Cargill K.L., Pyle B.H., Sauer R.L., McFeters G.A. Effect of culture conditions and biofilm formation on the iodine susceptibility of Legionella pneumophila. Can. J. Microbiol. 38:1992;423-429.
7. Pyle B.H., McFeters G.A. Iodine susceptibility of pseudomonads attached to stainless steel surfaces. Biofouling. 2:1990;113-120.
8. LeChavallier M.W., Cawthon C.D., Lee R.G. Inactivation of biofilm bacteria. Appl. Environ. Microbiol. 54:1988;2492-2499.
9. Anwar H., Strap J.L., Costerton J.W. Growth characteristics and expression of iron-regulated outer-membrane proteins of chemostat-grown biofilm cells of Pseudomonas aeruginosa. Can. J. Microbiol. 37:1991;737-743.
10. Cozens R.M., Tuomanen E., Tosch W., Zak O., Suter J., Toomasz A. Evaluation of the bactericidal activity of β-lactam antibiotics upon slowly growing bacteria cultured in a chemostat. Antimicrob. Agents Chemother. 29:1986;797-802.
11. Ombaka E.A., Cozens R.M., Brown M.R.W. Influence of nutrient limitation of growth on stability and production of virulence factors of mucoid and nonmucoid strains of Pseudomonas aeruginosa. Rev. Infect. Dis. 5(Suppl. 5):1983;S880-S888.
12. Gopalan R., Veeramani H. Studies on microbial chromate reduction by Pseudomonas sp. in aerobic continuous suspended growth cultures. Biotechnol. Bioeng. 43:1993;471-476.
13. Senn H., Lendenmann U., Snozzi M., Hamer G., Egli T. The growth of E. coli in glucose limited chemostat cultures: a re-examination of the kinetics. Biochem. Biophys. Acta. 1201:1994;424-436.
14. M. Whiteley, R.J.C. McLean, J.R. Ott, Interactions of community composition and carbon limitation on biofilm populations and susceptibility to iodine disinfection, Appl. Environ. Microbiol. (1997) (submitted).
15. Johnston M.D., Jones M.V. Disinfection tests with intact biofilms: combined use of the Modified Robbins Device with impedance detection. J. Microbiol. Methods. 21:1995;15-26.
16. - Pseudomonas fluorescens. Microb. Ecol. 18:1989;1-19.