Bacterial toxins block endothelial wound repair: Evidence that Rho GTPases control cytoskeletal rearrangements in migrating endothelial cells

Arteriosclerosis, Thrombosis, and Vascular Biology

Volumn 17, Issue 9, 1997, Pages 1623-1629

  Aepfelbacher, Martin ^a,c   Essler, Markus ^a   Huber, Elisabeth ^a   Sugai, Motoyuki ^b   Weber, Peter C ^a  

^a UNIVERSITY OF MUNICH   (Germany)

^b HIROSHIMA UNIVERSITY   (Japan)

^c Inst Prophylaxe Epidemiol K   (Germany)

Author keywords

ADP ribosylation; Bacterial toxins; Cell migration; Rho GTPase; Vascular endothelium

Indexed keywords

ACTIN; BACTERIAL TOXIN; CLOSTRIDIUM TOXIN; GLUCOSYLTRANSFERASE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATASE; NICOTINAMIDE ADENINE DINUCLEOTIDE ADENOSINE DIPHOSPHATE RIBOSYLTRANSFERASE; STAPHYLOCOCCUS TOXIN; VINCULIN;

ADENOSINE DIPHOSPHATE RIBOSYLATION; ARTICLE; CELL DAMAGE; CELL MIGRATION; CLOSTRIDIUM BOTULINUM; CLOSTRIDIUM DIFFICILE; CONTROLLED STUDY; CYTOSKELETON; ENDOTHELIUM CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; UMBILICAL VEIN; WOUND HEALING;

EID: 0030823317     PISSN: 10795642     EISSN: None     Source Type: Journal    
DOI: 10.1161/01.ATV.17.9.1623     Document Type: Article

References (19)

1. Schwartz SM, Haudenschild CC, Eddy EM. Endothelial regeneration, I: quantitative analysis of initial stages of endothelial regeneration in rat aortic intima. Lab Invest. 1978;38:568-580.
2. Haudenschild CC, Schwartz SM. Endothelial regeneration, II: restitution of endothelial continuity. Lab Invest. 1979;41:407-418.
3. Folkman J, Shing Y. Angiogenesis. J. Biol Chem. 1992;267:10931-10934.
4. Coomber BL, Gotlieb AI. In vitro endothelial wound repair: interaction of cell migration and proliferation. Arteriosclerosis. 1990;10: 215-222.
5. Sholley MM, Gimbrone MA, Cotran RS. Cellular migration and replication in endothelial regeneration: a study using irradiated endothelial cultures. Lab Invest. 1977;36:18-25.
6. Selden SC, Schwartz SM. Cytochalasin B inhibition of endothelial proliferation at wound edges in vitro. J Cell Biol. 1979;81:348-354.
7. Wong MKK, Gotlieb AI. In vitro reendothelialization of a single-cell wound: role of microfilament bundles in rapid lamellipodia-mediated wound closure. Lab Invest. 1984;51:75-81.
8. Romer LH, McLean N, Turner CE, Burridge K. Tyrosine kinase activity, cytoskeletal organization, and motility in human vascular endothelial cells. Mol Biol Cell. 1994;5:349-361.
9. Burridge K, Fath K, Kelly T, Nuckolls G, Turner C. Focal adhesions: transmembrane junctions between the extracellular matrix and the cytoskeleton. Annu Rev Cell Biol. 1988;4:487-525.
10. Huttenlocher A, Sandborg RR, Horwitz AF. Adhesion in cell migration. Curr Opin Cell Biol. 1995;7:697-706.
11. Hall A. Small GTP-binding proteins and the regulation of the actin cytoskeleton. Annu Rev Cell Biol. 1994;10:31-54.
12. Ridley AJ. Rho: theme and variations. Curr Biol. 1996;6:1256-1264.
13. Boguski MS, McCormick F. Proteins regulating ras and its relatives. Nature. 1993;366:643-654.
14. Aktories K, Just I. In vitro ADP-ribosylation of Rho by bacterial ADP-ribosyltransferases. Methods Enzymol. 1995;256:184-195.
15. Aktories K, Just I. Monoglucosylation of low-molecular mass GTP-binding Rho proteins by clostridial cytotoxins. Trends Cell Biol. 1995;5:441-443.
16. Ridley AJ, Hall A. The small GTP-binding protein rho regulates the assembly of focal adhesions and actin stress fibers in response to growth factors. Cell. 1992;70:389-399.
17. Smith DB, Johnson KS. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988;67:31-40.
18. Sugai M, Enomoto T, Hashimoto K, Matsumoto K, Matsuo Y, Ohgai H, Hong YM, Inoue S, Yoshikawa K, Suginawa H. A novel epidermal cell differentiation inhibitor (EDIN): purification and characterization from Staphylococcus aureus. Biochem Biophys Res Commun. 1990;173:92-98.
19. O'Farrell PH. High resolution two-dimensional electrophoresis of proteins. J Biol Chem. 1975;250:4007-4012.