Construction of a new leucine dehydrogenase with preferred specificity for NADP+ by site-directed mutagenesis of the strictly NAD+-specific enzyme

Protein Engineering

Volumn 10, Issue 6, 1997, Pages 687-690

  Galkin, Andrey ^a   Kulakova, Ljudmila ^a   Ohshima, Toshihisa ^b   Esaki, Nobuyoshi ^a   Soda, Kenji ^c  

^a KYOTO UNIVERSITY   (Japan)

^b UNIVERSITY OF TOKUSHIMA   (Japan)

^c KANSAI UNIVERSITY   (Japan)

Author keywords

Coenzyme binding site; Coenzyme specificity; Leucine dehydrogenase; NAD(P)+ specific enzyme

Indexed keywords

BACTERIAL ENZYME; LEUCINE DEHYDROGENASE; MUTANT PROTEIN; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; STREPTOMYCETACEAE; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS); FELIS CATUS; STREPTOMYCETACEAE; THERMOACTINOMYCES INTERMEDIUS;

EID: 0030822848     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.6.687     Document Type: Article

References (18)

1. Baker, P.J., Turnbull, A.P., Sedelnikova, S.E., Stillman, T.J. and Rice, D.W. (1995) Structure, 3, 693-705.
2. Bocanegra, J.A., Scrutton, N.S. and Perham, R.N. (1993) Biochemistry, 32, 2737-2740.
3. Britton, K.L., Baker, P.J., Rice, D.W. and Stillman, T.J. (1992) Eur. J. Biochem., 209, 851-859.
4. Britton, K.L., Baker, P.J., Paul, C.E., Rice, D.W. and Stillman, T.J. (1993) J. Mol. Biol. 234, 938-945.
5. Chen, Z., Lee, W.R. and Chang, S.H. (1991) Eur. J. Biochem., 202, 263-267.
6. Chen, R.D., Greer, A. and Dean, A.M. (1995) Proc. Natl Acad. Sci. USA, 92, 11666-11670.
7. Clermont, S., Corbier, C., Mely, Y., Gerard, D., Wanacott, A. and Branlant, G. (1993) Biochemistry, 32, 10178-10184.
8. Fan, F., Lorenzen, A.J. and Plapp, B.V. (1991) Biochemistry, 30, 6397-6401.
9. Feeney, R., Clarke, A.R. and Holbrook, J.J. (1990) Biochem. Biophys. Res. Commun., 166, 667-672.
10. Fersht, A. (1977) Enzyme Structure and Function. W.H. Freeman & Co., Reading, UK.
11. Haeffner-Gormley, L., Chen, Z., Zalkin, H. and Colman, R.F. (1992) Biochemistry, 31, 7807-7814.
12. Mittl, P.R.E., Berry, A., Scrutton, N.S., Perham, R.N. and Schulz, G.E. (1993) J. Mol. Biol., 231, 191-195.
13. Nagata, S., Tanizawa, K., Esaki, N., Sakamoto, Y., Ohshima, T., Tanaka, H. and Soda, K. (1988) Biochemistry, 27, 9056-9062.
14. Nishiyama, M., Birktoft, J.J. and Beppu, T. (1993) J. Biol. Chem., 268, 4656-4660.
15. Ohshima, T., Nishida, N., Bakthavatsalam, S., Kataoka, K., Takada, H., Yoshimura, T., Esaki, N. and Soda, K. (1994) Eur. J. Biochem., 222, 305-312.
16. Scrutton, N.S., Berry, A. and Perham, R.N. (1990) Nature, 343, 38-43.
17. Sem, D.S. and Kasper, C.B. (1993) Biochemistry, 32, 11548-11558.
18. Yaoi, T., Miyazaki, K., Oshima, J., Komukai, Y. and Go, M. (1996) J. Biochem., 119, 1014-1018.