The Na+-specific interaction between the LysR-type regulator, NhaR, and the nhaA gene encoding the Na+/H+ antiporter of Escherichia coli

EMBO Journal

Volumn 16, Issue 19, 1997, Pages 5922-5929

  Carmel, O ^a   Rahav Manor, O ^a   Dover, N ^a   Shaanan, B ^a   Padan, E ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

Na+ specific transcription regulation; Na+ H+ antiporters; nhaA Na+ specific footprint; NhaR

Indexed keywords

ANTIPORTER; BACTERIAL DNA; CIS ACTING ELEMENT; DIMETHYL SULFATE; POTASSIUM ION; REGULATOR PROTEIN; SODIUM ION;

ARTICLE; BACTERIAL GENE; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DNA FOOTPRINTING; DNA METHYLATION; ESCHERICHIA COLI; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; SIGNAL TRANSDUCTION;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BINDING SITES; DNA FOOTPRINTING; DNA METHYLATION; DNA, BACTERIAL; DNA-BINDING PROTEINS; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE DELETION; HISTIDINE; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); SODIUM; SODIUM-HYDROGEN ANTIPORTER; TRANSCRIPTION FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0030822671     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/16.19.5922     Document Type: Article

References (30)

1. + antiporter gene in Escherichia coli. EMBO J., 13, 1981-1989.
2. Christman,M.F., Storz,G. and Ames,B.N. (1989) OxyR, a positive regulator of hydrogen peroxide-inducible genes in Escherichia coli and Salmonella typhimurium. is homologous to a family of bacterial regulatory proteins. Proc. Natl Acad. Sci. USA, 86, 3484-3488.
3. + antiporter in Escherichia coli, is positively regulated by nhaR and affected by hns. J. Bacteriol, 178, 6508-6517.
4. Galas,D.J. and Schmitz,A. (1978) DNase footprint: a simple method for the detection of protein-DNA binding specificity. Nucleic Acids. Res., 9, 6505-6525.
5. Glaser,G., Sarmientos,P and Cashel,M. (1983) Functional interrelationship between two tandem E.coli ribosomal RNA promoters. Nature, 302, 74-76.
6. Goethals,K., Van Motagu,M. and Holsters,M. (1992) Conserved motifs in a divergent nod box of Azorhizobium caulinodans ORS571 reveal a common structure in promoters regulated by LysR-type proteins. Proc. Natl Acad. Sci. USA, 89, 1646-1650.
7. + antiporter gene of E.coli. Proc. Natl Acad. Sci. USA, 84, 2615-2619.
8. + in a ΔμH+-independent fashion. J. Biol Chem., 270, 3816-3822.
9. Henikoff,S., Haughn,G.W., Calvo,J.M. and Wallace,J.C. (1988) A large family of bacterial activator proteins. Proc. Natl Acad. Sci. USA, 85, 6602-6606.
10. Karpel,R. (1990) PhD Thesis, Hebrew University, Jerusalem, Israel.
11. + antiporter activity in E.coli. J. Biol. Chem., 263, 10408-10414.
12. + ions. J. Biol. Chem., 266, 21753-21759.
13. Kullik,I., Stevens,J., Toledano,M.B. and Storz,G. (1995) Mutational analysis of the redox-sensitive transcriptional regulator OxyR: regions important for DNA binding and multimerization. J. Bacteriol., 177, 1285-1291.
14. Lowry,O.H., Rosebrough,N.J., Farr,A.L. and Randall,R.J. (1951) Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.
15. +-translocating ATPase in Enterococcus hirae. J. Biol. Chem., 271, 23661-23666.
16. Owen-Hughes,T.A., Pavitt,G.D., Santos,D.S., Sidebotham,J.M., Hulton,C.S.J., Hinton,J.C.D. and Higgins,C.F. (1992) The chromatin-associated protein H-NS interacts with curved DNA to influence DNA topology and gene expression. Cell, 71, 255-265.
17. + transport systems in prokaryotes. In Bakker,E. (ed.), Alkali Cation Transport Systems in Prokaryotes. CRC Press, Boca Raton, FL, pp. 3-24.
18. + in cells. Biochim. Biophys. Acta, 1185, 129-151.
19. + antiportersmolecular biology, biochemistry and physiology. In Konings.W.N., Kaback,H.R. and Lolkema,J. (eds), Handbook of Biological Physics, Vol. II, Transport Processes in Eukaryotic and Prokaryotic Organisms. Elsevier Science, The Netherlands, pp. 501-531.
20. + antiporter system(s). J. Biol. Chem., 264, 20297-20302.
21. Pan,J.W. and Macnab,R.M. (1990) Steady-state measurements of sodium and proton potentials at alkaline pH support the hypothesis of electrogenic antiport. J. Biol. Chem., 265, 9247-9250
22. Rahav-Manor,O., CarmeLO., Karpel.R., Taglicht,JX, Glaser,G., Schuldiner,S. and Padan,E. (1992) NhaR. a protein homologous to a family of bacterial regulatory proteins (LysR) regulates nhaA. the sodium proton antiporter gene in Escherichia coli. J. Biol. Chem., 267, 10433-10438.
23. Sasse-Dwight,S. and Gralla,J.D. (1991) Footprinting protein-DNA complexes in vivo. Methods Enzymol., 208, 146-168.
24. Schell,M.A. (1993) Molecular biology of the LysR family of transcriptional regulators. Annu. Rev. Microbiol., 47, 597-626.
25. Storz,G., Tartaglia,L.A. and Ames,B.N. (1990) Transcriptional regulator of oxidative stress-inducible genes: direct activation by oxidation. Science, 248, 189-194.
26. Tabor,S. and Richardson,C. (1985) A bacteriophage T7 RNA polymerase/ promoter system for controlled exclusive expression of specific genes. Proc. Natl Acad. Sci. USA, 82, 1074-1078.
27. Tartaglia,L.A., Stortz,G. and Ames,B.N. (1989) Identification and molecular analysis of oxyR-regulated promoters important for the bacterial adaptation to oxidative stress. J. Mol. Biol., 210, 709-719.
28. Toledano,M.B., Kullik,I., Trinh,R, Baird,P.T., Schneider,T.D. and Storz,G. (1994) Redox-dependent shift of OxyR-DNA contacts along an extended DNA-binding site: a mechanism for differential promoter selection. Cell. 78, 397-909.
29. Tupper,A.E., Owen-Hughes,T.A., Ussery,D.W., Santos,D.S., Ferguson, D.J.P., Sidebotham,J.M., Hinton,J.C.D. and Higgins,C.F. (1994) The chromatin-associated protein H-NS alters DNA topology in vitro. EMBO J., 13, 258-268.
30. Ussery,D.W. et al. (1994) The chromatin-associated protein H-NS. Biochimie, 76, 968-980.