Subnuclear localization of S/MAR-binding proteins is differently affected by in vitro stabilization with heat or Cu2+

Chromosoma

Volumn 106, Issue 2, 1997, Pages 81-93

  Neri, Luca M ^a,b   Fackelmayer, Frank O ^c   Zweyer, Marina ^e   Kohwi Shigematsu, Terumi ^d   Martelli, Alberto M ^e  

^a UNIVERSITY OF FERRARA   (Italy)

^b RIZZOLI ORTHOPAEDIC INSTITUTE   (Italy)

^c UNIVERSITY OF KONSTANZ   (Germany)

^d UNIVERSITY OF CALIFORNIA   (United States)

^e UNIVERSITY OF TRIESTE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

3,5 DIIODOSALICYLIC ACID; BINDING PROTEIN; COPPER ION; DNA TOPOISOMERASE (ATP HYDROLYSING); LITHIUM; NUCLEAR PROTEIN; POLYPEPTIDE; SODIUM CHLORIDE; SPERMIDINE; SPERMINE;

ARTICLE; CELL NUCLEUS MATRIX; CELL STRAIN K 562; CONTROLLED STUDY; HEAT TREATMENT; HUMAN; HUMAN CELL; PROTEIN STABILITY;

ANTIGENS, NEOPLASM; ANTIGENS, NUCLEAR; ARTIFACTS; CATIONS, DIVALENT; CELL NUCLEUS; COPPER; DNA TOPOISOMERASES, TYPE II; DNA TOPOISOMERASES, TYPE II, EUKARYOTIC; DNA-BINDING PROTEINS; HEAT; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEIN U; HETEROGENEOUS-NUCLEAR RIBONUCLEOPROTEINS; HISTOCYTOLOGICAL PREPARATION TECHNIQUES; HUMANS; ISOENZYMES; LASERS; LEUKEMIA, ERYTHROBLASTIC, ACUTE; MATRIX ATTACHMENT REGION BINDING PROTEINS; MICROSCOPY, CONFOCAL; NUCLEAR MATRIX; NUCLEAR MATRIX-ASSOCIATED PROTEINS; NUCLEAR PROTEINS; PROTEIN BINDING; RIBONUCLEOPROTEINS; TUMOR CELLS, CULTURED;

EUKARYOTA;

EID: 0030821816     PISSN: 00095915     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004120050227     Document Type: Article

References (67)

1. Adachi Y, Kas E, Laemmli UK (1989) Preferential, cooperative binding of DNA topoisomerase II to scaffold-associated regions. EMBO J 13:3997-4006
2. Belgrader P, Siegel AJ, Berezney R (1991) A comprehensive study on the isolation and characterization of the HeLa S3 nuclear matrix. J Cell Sci 98:281-291
3. Berezney R (1991) The nuclear matrix: a heuristic model for investigating genomic organization and function in the cell nucleus. J Cell Biochem 47:109-123
4. Berezney R, Coffey DS (1974) Identification of a nuclear protein matrix. Biochem Biophys Res Commun 60:1410-1417
5. Berezney R, Mortillaro MJ, Ma H, Wei X, Samarabandu J (1995) The nuclear matrix: a structural milieu for genomic function. Int Rev Cytol 162A:1-65
6. Blencowe B, Nickerson JA, Issner R, Penman S, Sharp PA (1994) Association of nuclear matrix antigens with exon-containing splicing complexes. J Cell Biol 127:593-607
7. Boulikas T (1995) Chromatin domains and prediction of MAR sequences. Int Rev Cytol 162A:279-388
8. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72:248-254
9. Carter KC, Taneja KL, Lawrence JB (1991) Discrete nuclear domains of poly(A) RNA and their relationship to the functional organization of the nucleus. J Cell Biol 115:1191-1202
10. Cockerill PN, Garrard WT (1986) Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites. Cell 44:273-282
11. Cook PR (1988) The nucleoskeleton: artefact, passive framework or active site? J Cell Sci 90:1-6
12. Aγ-globin binds to the nuclear matrix and interacts with special A-T-rich binding protein 1 (SATB1), an SAR/MAR-associating region DNA binding protein. Blood 84:1298-1308
13. de Graaf A, Meijne AML, van Renswoude JBM, Humbel BM, van Bergen en Henegouwen PMP, de Jong L, van Driel R, Verkleij AJ (1992) Heat shock-induced redistribution of a 160-kDa nuclear matrix protein. Exp Cell Res 202:243-251
14. Dickinson LA, Kohwi-Shigematsu T (1995) Nucleolin is a matrix attachment region DNA-binding protein that specifically recognizes a region with high base-unpairing potential. Mol Cell Biol 15:456-465
15. Dickinson LA, Joh T, Kohwi Y, Kohwi-Shigematsu T (1992) A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition. Cell 70:631-645
16. c-myc as a member of a discrete subset of nuclear proteins. Cell 43:253-261
17. Fackelmayer FO, Richter A (1994) Purification of two isoforms of hnRNP-U and characterization of their nucleic acid binding activity. Biochemistry 33:10416-10422
18. Fackelmayer FO. Dahm K, Renz A, Ramsperger U, Richter A (1994) Nucleic-acid-binding properties of hnRNP-U/SAF-A., a nuclear-matrix protein which binds DNA and RNA in vivo and in vitro. Eur J Biochem 221:749-757
19. Ferraro A, Eufemi M, Cervoni L, Turano C (1995) The binding of DNA to the nuclear matrix studied by cross-linking reactions. J Cell Biochem 21B:134
20. Ferraro A, Cervoni L, Eufemi M, Turano C (1996) Comparison of DNA-protein interactions in intact nuclei from avian liver and erythrocytes: a cross-linking study. J Cell Biochem 62:495-505
21. Fischer D, Hock R, Scheer U (1993) DNA topoisomerase II is not detectable on lampbrush chromosomes but enriched in the amplified nucleoli of Xenopus oocytes. Exp Cell Res 209:255-260
22. Fisher PA, Lin L, McConnell M, Greenleaf A, Lee J-M, Smith DE (1989) Heat shock-induced appearance of RNA polymerase II in karyoskeletal protein-enriched (nuclear "matrix") fractions correlates with transcriptional shutdown in Drosophila melanogaster. J Biol Chem 264:3464-3469
23. Gasser SM, Laemmli UK (1986) The organisation of chromatin loops: characterization of a scaffold attachment site. EMBO J 5:511-518
24. Gasser SM, Laemmli UK (1987) Improved methods for the isolation of individual and clustered mitotic chromosomes. Exp Cell Res 173:85-98
25. Hempel K, Stratling WH (1996) The chicken lysozyme gene 5′ MAR and the Drosophila histone SAR are electroelutable from encapsulated and digested nuclei. J Cell Sci 109:1459-1469
26. Hirano T, Mitchison TJ (1993) Topoisomerase II does not play a scaffolding role in the organization of mitotic chromosomes assembled in Xenopus egg extracts J Cell Biol 120:601-612
27. Izaurralde E, Mirkovitch J, Laemmli UK (1988) Interaction of DNA with nuclear scaffolds in vitro. J Mol Biol 200:111-125
28. Izaurralde E, Kas E, Laemmli UK (1989) Highly preferential nucleation of histone Hl assembly on scaffold-associated regions. J Mol Biol 210:573-585
29. Jack RS, Eggert H (1992) The elusive nuclear matrix. Eur J Biochem. 209:503-509
30. Jackson DA, Cook PR (1995) The structural basis of nuclear funcion. Int Rev Cytol 162A:125-149
31. Jackson DA, Dickinson P, Cook PR (1990a) The size ot chromatin loops in HeLa cells. EMBO J 9:567-571
32. Jackson DA, Dickinson P, Cook PR (1990b) Attachment of DNA to the nucleoskeleton of HeLa cells examined using physiological conditions. Nucleic Acids Res 18:4385-4393
33. Jackson DA, Balajee AS, Mullenders L, Cook PR (1994) Sites in human nuclei where DNA damaged by ultraviolet light is repaired: visualization and localization relative to the nucleoskeleton. J Cell Sci 107:1745-1752
34. Kaufmann SH, Coffey DS, Shaper JH (1981) Considerations in the isolation of rat liver nuclear matrix, nuclear envelope, and pore complex lamina. Exp Cell Res 132:105-123
35. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the bacteriophage T4. Nature 227:680-685
36. Lavai M, Bouteille M (1973) Synthetic activity of isolated rat liver nuclei. I. Ultrastructural study at various steps of isolation. Exp Cell Res 76:337-348
37. Lebkowski JS, Laemmli UK (1982) Non-histone proteins and long-range organization of HeLa interphase DNA. J Mol Biol 156:325-344
38. Lewis CD, Lebkowski JS, Daly AK, Laemmli UK (1984) Interphase nuclear matrix and metaphase scaffolding structures. J Cell Sci Suppl 1:103-122
39. Littlewood TD, Hancock DC, Evan GI (1987) Characterization of a heat shock-induced insoluble complex in the nuclei of cells. J Cell Sci 88:65-72
40. 1. Cell 70:949-959
41. Ludérus ME, den Blauween JL, de Smit OJ, Compton DA, van Driel R (1994) Binding of matrix attachment regions to lamin polymers involves single-stranded regions and the minor groove. Mol Cell Biol 14:6297-6305
42. Martelli AM, Falcieri E, Gobbi P, Manzoli L, Gilmour RS, Cocco L (1991) Heat-induced stabilization of the nuclear matrix. A biochemical and morphological analysis in mouse erythroleukemia cells. Exp Cell Res 196:216-225
43. Martelli AM, Manzoli L, Rubbini S, Billi AM, Bareggi R, Cocco L (1995) The protein composition of Friend cell nuclear matrix stabilized by various treatments. Different recovery of nucleolar proteins B23 and C23 and nuclear lamins. Biol Cell 83:15-22
44. Martelli AM, Cocco L, Riederer BM, Neri LM (1996) The nuclear matrix: a critical appraisal. Histol Histopathol 11:1035-1048
45. Mirkovitch J, Mirault M-E, Laemmli UK (1984) Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell 39:223-232
46. Munro HN, Fleck A (1965) The determination of nucleic acids. Methods Biochem Anal 14:113-176
47. Nakayasu H, Berezney R (1989) Mapping replicational sites in the eucaryotic cell nucleus. J Cell Biol 108:1-11
48. Neri LM, Santi S, Marugg RA , Riederer BM, Capitani S, Cataldi A, Martelli AM (1994) In vitro heat exposure induces a redistribution of nuclear matrix proteins in K562 erythroleukemia cells. Exp Cell Res 213:275-285
49. Neri LM, Riederer BM, Marugg RA, Capitani S, Martelli AM (1995a) Analysis by confocal microscopy of the behavior of heat shock protein 70 within the nucleus and of a nuclear matrix polypeptide during prolonged heat shock response in HeLa cells. Exp Cell Res 221:301-310
50. Neri LM, Riederer BM, Marugg RA, Capitani S, Martelli AM (1995b) The effect of sodium tetrathionate stabilization on the distribution of three nuclear matrix proteins in human K562 erythroleukemia cells. Histochem Cell Biol 104:29-36
51. Ornelles DA, Penman S (1990) Prompt heat-shock and heat-shifted proteins associated with the nuclear matrix-intermediate filament scaffold in Drosophila melanogaster cells. J Cell Sci 95:393-404
52. Phi-Van L, Stratling WH (1988) The matrix attachment regions of the chicken lysozyme gene co-map with the boundaries of the chromatin domain. EMBO J 7:655-664
53. Razin SV, Vassetzky YS, Hancock R (1991a) Nuclear matrix attachment regions and topoisomerase II binding and reaction sites in the vicinity of a chicken DNA replication origin. Biochem Biophys Res Commun 177:265-270
54. Razin SV, Petrov P, Hancock R (1991b) Precise localization of the α-globin gene cluster within one of the 20-to 300-kilobase DNA fragments released by cleavage of chicken chromosomal DNA at topoisomerase II sites in vivo: evidence that the fragments are DNA loops or domains. Proc Natl Acad Sci USA 88:8515-8519
55. Renz A, Fackelmayer FO (1996) Purification and molecular cloning of the scaffold attachment factor B (SAF-B), a novel human nuclear protein that specifically binds to S/MAR DNA. Nucleic Acids Res 24:843-849
56. Saredi A, Howard L, Compton DA (1996) NuMA assembles into an extensive filamentous structure when expressed in the cell cytoplasm. J Cell Sci 109:619-630
57. Strick R, Laemmli UK (1995) SARs are cis DNA elements of chromosome dynamics: synthesis of a SAR repressor protein. Cell 83:1137-1148
58. Stuurman N, de Jong L, van Driel R (1992) Nuclear frameworks: concepts and operational definitions. Cell Biol Int Rep 16:837-852
59. Tsutsui K, Tsutsui K, Okada S, Watarai S, Seki S, Yasidai T, Shohmori T (1993) Identification and characterization of a nuclear scaffold protein that binds the matrix attachment region DNA. J Biol Chem 268:12886-12894
60. von Kries JP, Buck F, Stratling WH (1994) Chicken MAR binding protein p120 is identical to human heterogeneous nuclear ribonucleoprotein (hnRNP) U. Nucleic Acids Res 22:1215-1220
61. Wachsberger PR, Coss RA (1994) Recovery of nuclear matrix ultrastructure of interphase CHO cells after heat shock. J Cell Physiol 160:97-106
62. Wansink DG, Schul W, van der Kraan I, van Steensel B, van Driel R, de Jong L (1993) Fluorescent labeling of nascent RNA reveals transcription by RNA polymerase II in domains scattered throughout the nucleus. J Cell Biol 122:283-293
63. Yang CH, Lambie EJ, Snyder M (1992) NuMA: an unusually long coiled-coil related protein in the mammalian cell nucleus. J Cell Biol 116:1303-1317
64. Zeng C, He D, Berget SM, Brinkley BR (1994a) Nuclear-mitotic apparatus protein: a structural protein interface between the nucleoskeleton and RNA splicing. Proc Natl Acad Sci USA 91:1505-1509
65. Zeng C, He D, Brinkley BR (1994b) Localization of NuMA protein isoforms in the nuclear matrix of mammalian cells. Cell Motil Cytoskel 29:167-176
66. Zhang C, Jenkins H, Goldberg MW, Ailed TD, Hutchison CJ (1996) Nuclear lamina and nuclear matrix organization in sperm pronuclei assembled in Xenopus egg extract. J Cell Sci 109:2275-2286
67. Zhang G, Taneja KL, Singer RH, Green MR (1994) Localization of pre-mRNA splicing in mammalian nuclei. Nature 372:809-812