A G protein-coupled receptor phosphatase required for rhodopsin function

Science

Volumn 277, Issue 5326, 1997, Pages 687-690

  Vinós, Javier ^a   Jalink, Kees ^a   Hardy, Robert W ^a   Britt, Steven G ^a,b   Zuker, Charles S ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b Mays Cancer Center at UT Health San Antonio   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; PHOSPHOPROTEIN PHOSPHATASE; RHODOPSIN;

ANIMAL TISSUE; ARTICLE; DEPHOSPHORYLATION; DROSOPHILA; NONHUMAN; PHOTOSTIMULATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; RETINA DEGENERATION; SIGNAL TRANSDUCTION;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; ARRESTIN; CALCIUM-BINDING PROTEINS; DARKNESS; DROSOPHILA; DROSOPHILA PROTEINS; ELECTRORETINOGRAPHY; GTP-BINDING PROTEINS; LIGHT; MUTATION; PHOSPHOPROTEIN PHOSPHATASE; PHOSPHORYLATION; PHOTORECEPTORS, INVERTEBRATE; RETINA; RETINAL DEGENERATION; RHODOPSIN; SIGNAL TRANSDUCTION;

ANIMALIA;

EID: 0030820848     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5326.687     Document Type: Article

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58. ERGs of 4-day-old adult, dark-raised flies were recorded as described (32). Stimulating light was delivered with a 450-W xenon lamp (Osram) and filtered with either a 480-nm bandpass filter (Oriel, 53850) for blue light or a 570-nm longpass filter (Oriel, 51310) for orange light. Recordings (Fig. 3A) were performed with orange light attenuated 1 : 1585. Similar responses were elicited with blue light. For intracellular recordings, a coronal section was made through the compound eye. Dorsal hemispheres of the head were mounted upside down and immediately immersed in Schneider's medium (Gibco). Photoreceptor cells were impaled with 90-to 150-MΩ electrodes filled with 2 M KCI. Maximal differences in deactivation time were found when stimuli 0.5 s or longer were used. Under whole-cell recording conditions, no significant differences in deactivation time were found.
59. We thank P. Dolph for suggestions and fly stocks, and A. Newton and members of the Zuker laboratory for critical reading of the manuscript. K.J. is an American Cancer Society junior fellow, R.W.H. is an associate of the Howard Hughes Medical Institute, and C.S.Z. is an investigator of the Howard Hughes Medical Institute. Supported in part by a grant from the National Eye Institute.