Detection of Ki-ras mutations by PCR and differential hybridization and of p53 mutations by SSCP analysis in endoscopically obtained lavage solution from patients with long-standing ulcerative colitis

American Journal of Gastroenterology

Volumn 92, Issue 12 SUPPL., 1997, Pages 2166-2170

  Lang, S M ^a   Heinzlmann, M ^a   Stratakis, D F ^a   Teschauer, W ^a   Loeschke, K ^a  

^a LUDWIG MAXIMILIANS UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN P53;

ARTICLE; CLINICAL ARTICLE; COLON LAVAGE; COLONOSCOPY; DNA DETERMINATION; DNA HYBRIDIZATION; FEMALE; GENE MUTATION; HUMAN; MALE; ONCOGENE K RAS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM; ULCERATIVE COLITIS;

ASPARTIC ACID; BIOPSY; CASE-CONTROL STUDIES; CELL TRANSFORMATION, NEOPLASTIC; CODON; COLITIS, ULCERATIVE; COLONIC NEOPLASMS; COLONOSCOPY; DIGOXIGENIN; DNA; EXONS; FEMALE; GENES, P53; GENES, RAS; GENETIC SCREENING; GLYCINE; HUMANS; IMMUNOBLOTTING; IRRIGATION; MALE; MUTATION; NUCLEIC ACID HYBRIDIZATION; POINT MUTATION; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; VALINE;

EID: 0030819251     PISSN: 00029270     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (32)

1. Ekbom A, Helmick C, Zack M, et al. Ulcerative colitis and colorectal cancer. N Engl J Med 1990;323:1228-33.
2. Ekbom A. Chronic ulcerative colitis and Crohn's disease as precancerous conditions: What is the role of colonoscopy? Endoscopy 1995; 27:50-3.
3. Lennard-Jones JE, Melville DM, Morson BC, et al. Precancer and cancer in extensive ulcerative colitis: Findings among 401 patients over 22 years. Gut 1990;31:800-6.
4. Lashner BA, Silverstein MD, Hanauer SB. Hazard rates for dysplasia and cancer in ulcerative colitis: Results from a surveillance program. Dig Dis Sci 1989;34:1536-41.
5. Choi PM, Nugent FW, Schoetz DJ, et al. Colonoscopic surveillance reduces mortality from colorectal cancer in ulcerative colitis. Gastroenterology 1993;105:418-24.
6. Blackstone MO, Riddell RH, Rogers BH, et al. Dysplasia-associated lesion or mass (DALM) detected by colonoscopy in long-standing ulcerative colitis: An indication for colectomy. Gastroenterology 1981; 80:366-74.
7. Löfberg R. Latest views on cancer in inflammatory bowel disease. Res Clin Forum 1994;17:1:19-28.
8. Lennard-Jones JE. Colitic cancer: Supervision, surveillance, or surgery? Gastroenterology 1995;109:1388-91.
9. Van Laethem JL. Use of genetic markers during endoscopic screening and follow-up of gastrointestinal precancerous lesions. Acta Gastroenterol Belg 1995;58:187-92.
10. Vogelstein B, Fearon ER, Hamilton SR, et al. Genetic alterations during colorectal tumor development. N Engl J Med 1988;319:525-32.
11. Duffy MJ. Cellular oncogens and suppressor genes as prognostic markers in cancer. Clin Biochem 1993;26:439-47.
12. Bell SM, Kelly SA, Hoyle JA, et al. c-Ki-ras gene mutations in dysplasia and carcinomas complicating ulcerative colitis. Br J Cancer 1991;64:174-8.
13. Benhattar J, Saraga E. Molecular genetics of dysplasia in ulcerative colitis. Eur J Cancer 1995;31A:1171-3.
14. Greenwald BD, Harpaz N, Yin J, et al. Loss of heterozygosity affecting the p53, Rb, and mcc/apc tumor suppressor gene loci in dysplastic and cancerous ulcerative colitis. Cancer Res 1992;52:741-5.
15. Goh H-S, Yao J, Smith DR. p53 point mutation and survival in colorectal cancer patients. Cancer Res 1995;55:5217-21.
16. Williams AC, Browne SJ, Manning AM, et al. Biological consequences of the genetic changes which occur during human colorectal carcinogenesis. Semin Cancer Biol 1993;4:153-9.
17. Kern SE, Redston M, Seymour AB, et al. Molecular genetic profiles of colitis-associated neoplasms. Gastroenterology 1994;107:420-8.
18. Redston MS, Papadopoulos N, Caldas C, et al. Common occurrence of APC and Ki-ras gene mutations in the spectrum of colitis-associated neoplasms. Gastroenterology 1995;108:383-92.
19. Sidransky D, Tokino T, Hamilton SR, et al. Identification of ras oncogene mutations in the stool of patients with curable colorectal tumors. Science 1992;256:102-5.
20. Koomstra JJ, Rokke O, Halvorsen et al. Determination of the activated proto-oncogene (Ki-ras) in faeces: A new laboratory analysis for early diagnosis of colorectal cancer. Tidsskr Nor Laegeforen 1995;115:3266-70.
21. Villa E, Dugani A, Rebecchi AM, et al. Identification of subjects at risk for colorectal carcinoma through a test based on Ki-ras determination in the stool. Gastroenterology 1996;110:1346-53.
22. Nollau P, Moser C, Weinland G, et al. Detection of Ki-ras mutatons in stools of patients with colorectal cancer by mutant-enriched PCR. Int J Cancer 1996;66:332-6.
23. Smith-Ravin J, England J, Talbot IC, et al. Detection of c-Ki-ras in faecal samples from sporadic colorectal cancer patients. Gut 1995;36:81-6.
24. Eguchi S, Kohara N, Komuta K, et al. Mutations of the p53 gene in the stool of patients with resectable colorectal cancer. Cancer 1996;77: 1707-10.
25. Tobi M, Luo FC, Ronai Z. Detection of Ki-ras mutation in colonic effluent samples from patients without evidence of colorectal carcinoma. J Natl Cancer Inst 1994;86:1007-10.
26. Riesner D, Steger G, Zimmat R, et al. Temperature-gradient gel electrophoresis of nucleic acids: Analysis of conformational transitions, sequence variations, and protein-nucleic acid interactions. Electrophoresis 1989;10:37-89.
27. Boukamp P, Petrussevska RT, Breitkreuz D, et al. Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol 1988;106:761-71.
28. Lehman TA, Modali R, Boukamp P, et al. p53 mutations in human immortalized epithelial cell lines. Carcinogenesis 1993;14:833-9.
29. Teschauer W, Mussack T, Braun A, et al. Conditions for single strand conformation polymorphism (SSCP) analysis with broad applicability: A study on the effects of acrylamide, buffer and glycerol concentrations in SSCP analysis of exons of the p53 gene. Eur J Clin Chem Clin Biochem 1996;34:125-31.
30. Katzka I, Brody RS, Morris E, et al. Assessment of colorectal cancer risk in patients with ulcerative colitis: Experience from a private practice. Gastroenterology 1983;85:22-9.
31. Ilyas M, Talbot IC. p53 Expression in ulcerative colitis: A longitudinal study. Gut 1995;37:802-4.
32. Chen PH, Lin SY, Wang CK, et al. "Hot spots" mutation analysis of p53 gene in gastrointestinal cancers by amplifications of naturally occurring and artificially created restriction sites. Clin Chem 1993;39: 2186-91.