Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells

Science

Volumn 277, Issue 5323, 1997, Pages 221-225

  Zwilling, Stefan ^a   Dieckmann, Andreas ^a   Pfisterer, Petra ^a   Angel, Peter ^a   Wirth, Thomas ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCRIPTION FACTOR;

ARTICLE; B LYMPHOCYTE; CELL SPECIFICITY; CONTROLLED STUDY; GENE ACTIVATION; GENE EXPRESSION REGULATION; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; T LYMPHOCYTE; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; ANIMALS; B-LYMPHOCYTES; CELLS, CULTURED; DNA-BINDING PROTEINS; GENE EXPRESSION REGULATION; HELA CELLS; HOMEODOMAIN PROTEINS; HOST CELL FACTOR C1; HUMANS; IMMUNOSUPPRESSIVE AGENTS; IONOMYCIN; JURKAT CELLS; LYMPHOCYTE ACTIVATION; MICE; MOLECULAR SEQUENCE DATA; OCTAMER TRANSCRIPTION FACTOR-1; PHOSPHORYLATION; PHOSPHOSERINE; RECOMBINANT FUSION PROTEINS; T-LYMPHOCYTES; TETRADECANOYLPHORBOL ACETATE; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TRANSCRIPTION FACTORS;

MURINAE;

EID: 0030816433     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.277.5323.221     Document Type: Article

References (31)

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21. Total cytoplasmic RNA (10 μg) was analyzed as described (24). For protein immunoblots, 100 μg of whole-cell extracts were run on 12.5% SDS-polyacrylamide gels, transferred to polyvinylidene difluoride membranes, and probed with affinity-purified antibodies to BOB. 1/OBF. 1. Blots were developed with the ECL-system (Amersham) (8). When NIH/3T3 cells were analyzed, no increased BOB.1/OBF.1 RNA or protein expression was observed after PMA and ionomycin stimulation. Anisomycin (100 μM) and FK506 (1 ng/ml) inhibited the increase of BOB. 1/ OBF.1 RNA and protein expression.
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31. We thank Sandoz Pharma and Fujisawa Pharma for the gift of CsA and FK506, respectively. We also thank R. Röder for typing the manuscript and S. Pfränger for preparing the figures. Supported by grants from the Deutsche Forschungsgemeinschaft to T.W. (SFB 229 and SFB 465) and P.A. (An 182/7-1) and by Boehringer Ingelheim.