Advantages of orange-labelled collagen and gelatine as substrates for rapid collagenase activity measurement

Journal of Biochemical and Biophysical Methods

Volumn 34, Issue 3, 1997, Pages 237-249

  Komsa Penkova, Regina S ^a   Rashap, Rimma K ^b   Yomtova, Vihra M ^c  

^a MEDICAL UNIVERSITY PLEVEN   (Bulgaria)

^b Institute of Applied Enzymology   (Lithuania)

^c INSTITUTE OF ORGANIC CHEMISTRY   (Bulgaria)

Author keywords

Collagen orange; Collagenase activity; Gelatine orange; Labelled substrates

Indexed keywords

COLLAGEN; COLLAGENASE; GELATIN; IMMOBILIZED ENZYME;

ARTICLE; COLORIMETRY; ENZYME ASSAY; PRIORITY JOURNAL; TECHNIQUE;

BOVINAE;

EID: 0030815183     PISSN: 0165022X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-022X(97)00016-X     Document Type: Article

References (28)

1. B. Peterkofsky, (1982) Bacterial Collagenase, in: L.W. Cunningham and D.W. Frederiksen (Eds.), Methods in Enzymology, vol. 82, Academic Press, New York, 1982, pp. 453-471.
2. E.D.Jr. Harris and C.A. Vater, Vertebrate Collagenases, in: L.W Cunningham and D.W. Frederiksen (Eds.), Methods in Enzymology, vol. 82, Academic Press, New York, 1982, pp. 423-452.
3. Bleeg H.S.. Collagenolytic enzymes assayed by spectrophotometry with suspensions of reconstituted collagen fibrils. Conn. Tiss. Res. 1991;26:247-257.
4. Bergman G., Loxley R.. Two improved and simplified methods for the spectrophotometric determination of hydroxyproline. Anal. Chem. 1963;35:1961-1965.
5. Morales T.L., Woessner J.F., Howel D.S., Marsh J.M., LeMaire WJ.. A microassay for direct demonstration of collagenolytic activity in graafian follicles of the rat. Biochim. Biophys. Acta. 1978;524:428-434.
6. Mandl I., Zipper J.D.H., Ferguson L.T.. Clostridium histolyticum collagenase. Its purification and properties. Arch. Biochem. Biophys. 1958;74:465-475.
7. Rosen H.. A modified ninhydrin colorimetric analysis for amino acids. Arch. Biochem. Biophys. 1957;67:10-15.
8. Nethery A., Lyons G., O'Grady R.L.. A spectrophotometric collagenase assay. Anal. Biochem. 1986;159:390-395.
9. Bicsak T.A., Harper E.. Purification of nonspecific protease-free collagenase from Clostridium histolyticum. Anal. Biochem. 1985;145:286-291.
10. 14C]-acetylated collagen. Anal. Biochem. 1979;99:340-345.
11. Gisslow M.T., McBride B.C.. A rapid sensitive collagenase assay. Anal. Biochem. 1975;68:70-78.
12. Johnson-Wint B.. Quantitative collagen film collagenase assay for large number of samples. Anal. Biochem. 1980;104:175-180.
13. 3H] acetylated collagen. Anal. Biochem. 1985;148:174-181.
14. Ishikawa T., Nimmi M.E.. Modified collagenase assay method based on the use of p-dioxan. Anal. Biochem. 1979;92:136-143.
15. 2H]-furanone. Anal. Biochem. 1984;140:490-494.
16. Stack S.M., Grag R.D.. Comparison of vertebrate collagenase and gelatinase using a new fluorogenic substrate peptide . J. Biol. Chem. 1989;264:4277-4281.
17. Cooksley S., Hipkiss G.B., Tickle S.P., Holmes-Jevers E., Docherty A.G.P., Murphy G., Lowson A.D.G.. Immunoassays for detection of human collagenase, stromelysin, tissue inhibitor of metalloproteinases (TIMP) and enzyme-inhibitor complexes. Matrix. 1990;10:285-291.
18. Yoshioka H., Oyamada I., Usuku G.. An assay of collagenase activity using enzyme-linked immunosorbent assay for mammalian collagenase. Anal. Biochem. 1987;166:172-177.
19. Kasten M., Burhard H., van Roden H.-G., Raus S.. Spectroscopy collagenase assay using peroxidase labelled collagen. Anal. Biochem. 1989;176:150-156.
20. Vencil C.F., Rasnick D., Crumley K.V., Nishino N., Powers J.C.. Clostridium histolyticum collagenase: development of new thio ester, fluorogenic, and depsipeptide substrates and new inhibitors. Biochemistry. 1985;24:3149-3157.
21. Van Wart H.E., Steinbrink R.D.. A continuous spectrophotometric assay for Clostridium histolyticum collagenase . Anal. Biochem. 1981;113:356-365.
22. Wunsch E., Heidrich H.-G.Z.. Zur quantitativen Bestimmung der Kollagenase. Z. Physiol. Chem. 1963;333:149-151.
23. Chavira Jr. R., Burnett T.J., Hageman J.H.. Assaying proteinases with azocoll. Anal. Biochem. 1984;136:446-450.
24. R. Komsa-Penkova, A. Stojanova, and R. Rashap. Assessment of collagenase preparations, isolated from crab Paralithodes camchatica. Scientific Works of Medical University of Pleven, 15 (1), 1995, 8-11.
25. Chase Jr. T., Shaw E.. p-Nitrophenyl-p'-guanidinobenzoate.HCL: a new active site titration for trypsin. Biochem. Biophys. Res. Comm. 1967;29:508-514.
26. Schonbaum G.R., Zerner B., Bender M.L.. The spectrophotometric determination of the operational normality of an α-chymotrypsin. J. Biol. Chem. 1961;236:2930-2935.
27. Habeeb A.F.S.A.. Determination of free amino groups in proteins by trinitro-benzenesulfonic acid. Anal. Biochem. 1966;14:328-336.
28. H. Tschesche, Human Neutrophil Collagenase, in: J.A. Barret, (Ed.) Methods in Enzymology, Vol. 248, Academic Press, New York, 1995, pp. 431-449.