Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters

FEBS Letters

Volumn 413, Issue 2, 1997, Pages 194-196

  Ledent, Philippe ^a   Duez, Colette ^a   Vanhove, Marc ^a   Lejeune, Annabelle ^a   Fonzé, Eveline ^a   Charlier, Paulette ^a   Rhazi Filali, Fouzia ^c   Thamm, Iris ^a   Guillaume, Gilliane ^a   Samyn, Bart ^b   Devreese, Bart ^b   Van Beeumen, Jozef ^b   Lamotte Brasseur, Josette ^a   Frère, Jean Marie ^a  

^a UNIVERSITY OF LIÈGE   (Belgium)

^b Lab V E   (Belgium)

^c MOULAY ISMAIL UNIVERSITY   (Morocco)

Author keywords

Lactamase; Bacillus; His tag; Thermophile

Indexed keywords

BETA LACTAMASE; RECOMBINANT ENZYME;

ARTICLE; CRYSTALLOGRAPHY; ENZYME ACTIVITY; GENE CONSTRUCT; GENE EXPRESSION; GENETIC ENGINEERING; MASS SPECTROMETRY; MOLECULAR BIOLOGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; TECHNIQUE; THERMOPHILIC BACTERIUM;

BACILLUS; BETA-LACTAMASES; CRYSTALLOGRAPHY, X-RAY; HISTIDINE; KINETICS; MEMBRANE PROTEINS; PEPTIDES; PROTEIN FOLDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS; SERINE ENDOPEPTIDASES;

BACILLUS LICHENIFORMIS; BACTERIA (MICROORGANISMS);

EID: 0030811909     PISSN: 00145793     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0014-5793(97)00908-3     Document Type: Article

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