Cloning and expression in Escherichia coli of genetic determinants for production of and immunity to microcin E492 from Klebsiella pneumoniae

Journal of Bacteriology

Volumn 179, Issue 15, 1997, Pages 4789-4794

  Wilkens, Marcela ^a,b   Villanueva, Jorge E ^a   Cofré, Jaime ^a   Chnaiderman, Jonás ^a   Lagos, Rosalba ^a  

^a UNIVERSITY OF CHILE   (Chile)

^b UNIVERSIDAD DE SANTIAGO DE CHILE   (Chile)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBIOTIC AGENT; BACTERIAL DNA; MICROCIN; SIGMA FACTOR; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; ESCHERICHIA COLI; IMMUNITY; KLEBSIELLA PNEUMONIAE; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; KLEBSIELLA PNEUMONIAE;

EID: 0030811571     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.15.4789-4794.1997     Document Type: Article

References (26)

1. Abee, T. 1995. Pore-forming bacteriocins of Gram-positive bacteria and self-protection mechanisms of producer organism. FEMS Microbiol. Lett. 129:1-10.
2. Ausubel, F. M., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1992. Short protocols in molecular biology, 2nd ed. Greene Publishing Associates, New York, N.Y.
3. Baquero, F., and F. Moreno. 1984. The microcins. FEMS Microbiol. Lett. 23:117-124.
4. 70. J. Bacteriol. 173:4482-4492.
5. Cramer, W. A., J. B. Heymann, S. L. Schendel, B. N. Deriy, F. S. Cohen, P. A. Elkins, and C. V. Stauffacher. 1995. Structure-function of the channel-forming colicins. Annu. Rev. Biophys. Biomol. Struct. 24:611-641.
6. de Lorenzo, V. 1984. Isolation and characterization of microcin E492 from Klebsiella pneumoniae. Arch. Microbiol. 139:72-75.
7. de Lorenzo, V. 1985. Factors affecting microcin E492 production. J. Antibiot. 38:340-343.
8. de Lorenzo, V., J. L. Martínez, and C. Asensio. 1984. Microcin-mediated interactions between Klebsiella pneumoniae and Escherichia coli strains. J. Gen. Microbiol. 130:391-400.
9. de Lorenzo, V., and A. Pugsley. 1985. Microcin E492, a low-molecular-weight peptide antibiotic which causes depolarization of the Escherichia coli cytoplasmic membrane. Antimicrob. Agents Chemother. 27:666-669.
10. Fath, M. J., R. Skvirky, L. Gilson, H. K. Mahanty, and R. Kolter. 1992. The secretion of colicin V. p. 331-348. In R. James, C. Lazdunski, and F. Pattus (ed.), Bacteriocins, microcins, and lantibiotics. Springer Verlag, Heidelberg, Germany.
11. Gaggero, C., F. Moreno, and M. Laviña. 1993. Genetic analysis of microcin H47 antibiotic system. J. Bacteriol. 175:5420-5427.
12. Guarente, L., and J. Beckwith. 1978. Mutant RNA polymerase of Escherichia coli terminates transcription in strains making defective rho factor. Proc. Natl. Acad. Sci. USA 75:294-297.
13. Kolter, R., and F. Moreno. 1992. Genetics of ribosomally synthesized peptide antibiotics. Annu. Rev. Microbiol. 46:141-163.
14. Lagos, R., M. Wilkens, C. Vergara, X. Cecchi, and O. Monasterio. 1993. Microcin E492 forms ion channels in phospholipid bilayer membranes. FEBS Lett. 321:145-148.
15. Maniatis, T., E. F. Fritsch, and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
16. Mayr-Harting, A., A. J. Hedges, and C. W. Berkeley. 1972. Methods for studying bacteriocins. Methods Microbiol., 7A:315-422.
17. Miller, J. H. 1972. Experiments in molecular genetics, p. 431-433. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
18. Mulvey, M. R., J. Switala, A. Borys, and P. C. Loewen. 1990 Regulation of transcription of katE and katF in Escherichia coli. J. Bacteriol. 172:6713-6720.
19. Orellana, C., and R. Lagos. 1996. The activity of microcin E492 from Klebsiella pneumoniae is regulated by a microcin-antagonist. FEMS Microbiol. Lett. 136:297-303.
20. Ortiz, M., R. Lagos, and O. Monasterio. 1993. Interaction between the C-terminal peptides of tubulin and tubulin S detected with the fluorescent probe 4′6-diamidino-2-phenylindole. Arch. Biochem. Biophys. 303:159-164.
21. Pugsley, A. P., F. Moreno, and V. de Lorenzo. 1986. Microcin-E492-insensitive mutants of Escherichia coli K12. J. Gen. Microbiol. 132:3253-3259.
22. Schägger, H., and G. von Jagow. 1987. Tricinc-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
23. Studier, F. W., and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
24. Venema, K., G. Venema, and J. Kok. 1995. Lactococcal bacteriocins: mode of action and immunity. Trends Microbiol. 3:299-304.
25. Villanueva, J. E., and R. Lagos. Unpublished data.
26. Yang, C., and J. Konisky. 1984. Colicin V-tretead Escherichia coli does not generate membrane potential. J. Bacteriol. 158:757-759.