Transcriptional analysis of the Streptococcus mutans hrcA, grpE and dnaK genes and regulation of expression in response to heat shock and environmental acidification

Molecular Microbiology

Volumn 25, Issue 2, 1997, Pages 329-341

  Jayaraman, Gayatri C ^a,b   Penders, Jana E ^b   Burne, Robert A ^a,b  

^a UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

^b UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACIDIFICATION; ARTICLE; BACTERIAL GENE; GENE EXPRESSION; HEAT TOLERANCE; NONHUMAN; PRIORITY JOURNAL; STREPTOCOCCUS MUTANS; TRANSCRIPTION REGULATION;

BACTERIA (MICROORGANISMS); POSIBACTERIA; STREPTOCOCCUS MUTANS;

EID: 0030811569     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.1997.4671835.x     Document Type: Article

References (46)

1. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1987) Current Protocols in Molecular Microbiology. New York: John Wiley.
2. Belli, W.A., and Marquis, R.E. (1991) Adaptation of Streptococcus mutans and Enterococcus hirae to acid stress in continuous culture. Appl Environ Microbiol 57: 1134-1138.
3. Bender, G.R., and Marquis, R.E. (1987) Membrane ATPases and acid tolerance of Actinomyces viscosus and Lactobacillus casei. Appl Environ Microbiol 53: 2124-2128.
4. Bender, G.R., Sutton, S.V.W., and Marquis, R.E. (1986) Acid tolerance, proton permeabilities, and membrane ATPases of oral streptococci. Infect Immun 53: 331-338.
5. Bradford, M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.
6. Bukau, B. (1993) Regulation of the Escherichia coli heat shock response. Mol Microbiol 9: 671-680.
7. Burne, R.A., and Penders, J.E.C. (1992) Characterization of Streptococcus mutans GS-5 fruA gene encoding exo-β-D-fructosidase. Infect Immun 60: 4621-4632.
8. Burne, R.A., Rubinfeld, B., Bowen, W.H., and Yasbin, R.E. (1986) Tight genetic linkage of a glucosyltransferase and dextranase of Streptococcus mutans GS-5. Dent Res 65: 1392-1401.
9. Caufield, P.W., Childers, N.K., Allen, D.N., and Hansen, J.B. (1985) Distinct bacteriocin groups correlate with different groups of Streptococcus mutans plasmids. Infect Immun 48: 51-56.
10. Church, G.M., and Gilbert, W. (1984) Genomic sequencing. Proc Natl Acad Sci USA 81: 1991-1995.
11. Dashpur, S.G., and Reynolds, E.C. (1992) pH regulation by Streptococcus mutans. J Dent Res 71: 1159-1165.
12. Duncan, M.L., Kaiman, S.S., Thomas, S.M., Price, C.W. (1987) Gene encoding the 37000-dalton minor Sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function. J Bacteriol 169: 771-778.
13. Foster, J.W., and Hall, J.K. (1991) Inducible pH homeostasis and the acid tolerance response of Salmonella typhimurium. J Bacteriol 173: 5129-5135.
14. Glisin, V., Crkvenjakov, R., and Byus, C. (1974) Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13: 2633-2637.
15. Godson, G.N., and Vapne, D. (1973) A simple method of preparing large amounts of φX174 RFI supercoiled DNA. Biochim Biophys Acta 229: 516-520.
16. Haldenwang, W.G. (1995) The Sigma factors of Bacillus subtilis. Microbiol Rev 59: 1-30.
17. Hamilton, I.R., and Buckley, N.D. (1991) Adaptation by Streptococcus mutans to acid tolerance. Oral Microbiol Immunol 6: 65-71.
18. Hartl, F.U. (1996) Molecular chaperones in cellular protein folding. Nature 381: 571-580.
19. Hecker, M., Schumann, W., and Volker, U. (1996) Heat shock and general stress response in Bacillus subtilis. Mol Microbiol 19: 417-428.
20. Homuth, G., Masuda, S., Mogk, A., Kobayashi, Y., Schumann, W. (1997) The dnaK operon of Bacillus subtilis is heptacistronic. J Bacteriol 179: 1153-1164.
21. Hudson, M.C., and Curtiss III, R. (1990) Regulation of expression of Streptococcus mutans genes important to virulence. Infect Immun 58: 464-470.
22. Jayaraman, G.C., and Burne, R.A. (1995) DnaK expression in response to heat shock of Streptococcus mutans. FEMS Microbiol Lett 131: 255-261.
23. Kashket, E.R. (1987) Bioenergetics of lactic acid bacteria: cytoplasmic pH and osmotolerance. FEMS Microbiol Rev 46: 233-244.
24. Kruger, E., Msadek, T., and Hecker, M. (1996) Alternate promoters direct stress-induced transcription of the Bacillus subtilis clpC operon. Mol Microbiol 20: 713-723.
25. Le Blanc, D.J., Lee, L.N., and Abu-Al-Jaibat, A. (1992) Molecular, genetic and functional analysis of the basic replicon of pVA380-1, a plasmid of oral streptococcal origin. Plasmid 28: 130-145.
26. Lindquist, S., and Craig, E.A. (1988) The heat-shock proteins. Annu Rev Genet 22: 631-677.
27. Lipinska, B., King, J., Ang, D., and Georgopoulos, C. (1988) Sequence analysis and transcriptional regulation of the Escherichia coli grpE gene, encoding a heat shock protein. Nucleic Acids Res 16: 7545-7562.
28. Loesche, W.J. (1986) Role of Streptococcus mutans in human dental decay. Microbiol Rev 50: 353-380.
29. McCarthy, J.S., and Walker, G.C. (1991) DnaK as a thermometer: threonine-199 is site of autophosphorylation and is critical for ATPase activity. Proc Natl Acad Sci USA 88: 9513-9517.
30. McDonald, P.M., Kutter, E., and Mosig, G. (1984) Regulation of a bacteriophage T4 late gene, SOC, which maps in an early region. Genetics 106: 17-27.
31. Neidhardt, F.C., VanBogelen, R.A. (1987) Heat shock response. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology. Ingraham, J.L., Low, K.B., Magasanic, B., Schaechter, M., Umbarger, H.E. (eds). Washington DC: American Society for Microbiology Press, pp. 1334-1345.
32. Padan, E., Zilberstein, D., and Schuldiner, S. (1981) pH homeostasis in bacteria. Biochim Biophys Acta 650: 151-166.
33. Perry, D., Kuramitsu, H.K. (1990) Linkage of sucrose metabolizing genes in Streptococcus mutans. Infect Immun 58: 3462-3464.
34. Quivey, R.G., and Faustoferri, R.C. (1992) In vivo inactivation of the Streptococcus mutans recA gene mediated by PCR amplification and cloning of a recA DNA fragment. Gene 116: 35-42.
35. Quivey, R.G., Faustoferri, R.C., and Reyes, S.D. (1995) UV-resistance of acid-adapted Streptococcus mutans. In Genetics of streptocci, enterococci and lactococci. Ferretti, J.J., Gilmore, M.S., Klaenhammer, T.R., and Brown, F. (eds). Basle: Karger, pp. 393-398.
36. Rabile, H., Jayaraman, G.C., and Burne, R.A. (1996) Streptococcus mutans DnaK protein levels as a function of exposure to environmental Stressors. International Association of Dental Research Conference, Abstract 3131. 12-16 March, San Francisco, CA.
37. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press.
38. Sanger, F., Nicklen, S., and Coulsen, A.R. (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5436-5467.
39. Schulz, A., Tzschaschel, B., and Schumann, W. (1995) Isolation and analysis of mutants of the dnaK operon of Bacillus subtilis. Mol Microbiol 15: 421-429.
40. Shaw, W.V. (1979) Chloramphenicol acetyltransferase activity from chloramphenicol-resistant bacteria. Methods Enzymol 43: 737-755.
41. Spratt, B.G., Hedge, P.J., te Hessen, S., Edelman, A., and Broome-Smith, J.K. (1986) Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8, and pEMBL9. Gene 41: 337-342.
42. Volker, U., Mach, H., Schmid, R., and Hecker, M. (1992) Stress proteins and cross-protection by heat shock and salt stress in Bacillus subtilis. J Gen Microbiol 138: 2125-2135.
43. Volker, U., Volker, A., Maul, B., Hecker, M., Dufour, A., and Haldenwang, W.G. (1995) Separate mechanisms activate Sigma B of Bacillus subtilis in response to environmental and metabolic stress. J Bacteriol 177: 3771-3780.
44. Wetzstein, M., Volker, U., Dedio, J., Lobau, S., Zuber, U., Schiesswohl, M., Herget, C., Hecker, M., and Schumann, W. (1992) Cloning, sequencing, and molecular analysis of the dnaK locus from Bacillus subtilis. J Bacteriol 174: 3300-3310.
45. Wexler, D.L., Hudson, M.C., and Burne, R.A. (1993) Streptococcus mutans fructosyltransferase (ftf) and glucosyltransferase (gtfBC) operon fusions in continuous culture. Infect Immun 61: 1259-1267.
46. Yanisch-Peron, C., Vieira, J., and Messing, J. (1985) Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33: 103-119.