Temperature-sensitive lesions in the Francisella novicida valA gene cloned into an Escherichia coli msbA lpxK mutant affecting deoxycholate resistance and lipopolysaccharide assembly at the restrictive temperature

Journal of Bacteriology

Volumn 179, Issue 24, 1997, Pages 7638-7643

  Mcdonald, Megan K ^a   Cowley, Siobhán C ^a   Nano, Francis E ^a  

^a UNIVERSITY OF VICTORIA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

3 DEOXY MANNO OCTULOSONIC ACID; ABC TRANSPORTER; BACTERIUM LIPOPOLYSACCHARIDE; DEOXYCHOLIC ACID; EPITOPE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL OUTER MEMBRANE; CELL MEMBRANE TRANSPORT; CHLAMYDIA TRACHOMATIS; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; FRANCISELLA; GENE EXPRESSION; GENE LOCUS; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; TEMPERATURE SENSITIVE MUTANT;

BACTERIA (MICROORGANISMS); CHLAMYDIA TRACHOMATIS; ESCHERICHIA COLI; FRANCISELLA; FRANCISELLA TULARENSIS SUBSP. NOVICIDA;

EID: 0030811424     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.24.7638-7643.1997     Document Type: Article

References (23)

1. Brade, H., L. Brade, and F. E. Nano. 1987. Chemical and serological investigations on the genus-specific lipopolysaccharide epitope of Chlamydia. Proc. Natl. Acad. Sci. USA 84:2508-2512.
2. Bronner, D., V. Sieberth, C. Pazzani, A. Smith, G. Boulnois, I. Roberts, B. Jann, and K. Jann. 1993. Synthesis of the K5 (group II) capsular polysaccharide in transport-deficient recombinant Escherichia coli. FEMS Microbiol. Lett. 113:279-284.
3. Clementz, T., and C. R. H. Raetz. 1991. A gene coding for 3-deoxy-D-manno-octulosonic-acid transferase in Escherichia coli. J. Biol. Chem. 266:9687-9696.
4. Fath, M. J., and R. Kolter. 1993. ABC transporters: bacterial exporters. Microbiol. Rev. 57:995-1017.
5. Fu, Y., M. Baumann, P. Kosma, L. Brade, and H. Brade. 1992. A synthetic glycoconjugate representing the genus-specific epitope of chlamydial lipopolysaccharide exhibits the same specificity as its natural counterpart. Infect. Immun. 60:1314-1321.
6. Garrett, T. A., J. L. Kadrmas, and C. R. H. Raetz. 1997. Identification of the gene encoding the Escherichia coli lipid A 4′-kinase. J. Biol. Chem. 272: 21855-21864.
7. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-580.
8. Karow, M., O. Fayet, A. Cegielska, T. Ziegelhoffer, and C. Georgopoulos. 1991. Isolation and characterization of the Escherichia coli htrB gene, whose product is essential for bacterial viability above 33°C in rich media. J. Bacteriol. 173:741-750.
9. Karow, M., and C. Georgopoulos. 1993. The essential Escherichia coli msbA gene, a multicopy suppressor of null mutations in the htrB gene, is related to the universally conserved family of ATP-dependent translocators. Mol. Microbiol. 7:69-79.
10. Kröncke, K.-D., G. Boulnois, I. Roberts, D. Bitter-Suermann, J. R. Golecki, B. Jann, and K. Jann. 1990. Expression of the Escherichia coli K5 capsular antigen: immunoelectron microscopic and biochemical studies with recombinant E. coli. J. Bacteriol. 172:1085-1091.
11. Kröncke, K.-D., J. R. Golecki, and K. Jann. 1990. Further electron microscopic studies on the expression of Escherichia coli group II capsules. J. Bacteriol. 172:3469-3472.
12. Kurnit, D. M. 1989. Escherichia coli recA deletion strains that are highly competent for transformation and for in vivo phage packaging. Gene 82: 313-315.
13. Mdluli, K. E., L. S. D. Anthony, G. S. Baron, M. K. McDonald, S. V. Myltseva, and F. E. Nano. 1994. Serum-sensitive mutation of Francisella novicida: association with an ABC transporter gene. Microbiology 140:3309-3318.
14. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
15. Morales, V. M., A. Bäckman, and M. Bagdasarian. 1991. A series of widehost-range low-copy-number vectors that allow direct screening for recombinants. Gene 97:39-47.
16. Nano, F. E., and H. D. Caldwell. 1985. Expression of the chlamydial genus-specific lipopolysaccharide epitope in Escherichia coli. Science 228:742-744.
17. Osborn, M. J., J. E. Gander, E. Parisi, and J. Carson. 1972. Mechanism of assembly of the outer membrane of Salmonella typhimurium. J. Biol. Chem. 247:3962-3972.
18. Pavelka, M. S., Jr., L. F. Wright, and R. P. Silver. 1991. Identification of two genes, kpsM and kpsT, in region 3 of the polysialic acid gene cluster of Escherichia coli K1. J. Bacteriol. 173:4603-4610.
19. Polissi, A., and C. Georgopoulos. 1996. Mutational analysis and properties of the msbA gene of Escherichia coli, coding for an essential ABC family transporter. Mol. Microbiol. 20:1221-1233.
20. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
21. Spratt, B. G., P. J. Hedge, S. Heesen, A. Edelman, and J. K. Broome-Smith. 1986. Kanamycin-resistant vectors that are analogues of plasmids pUC8, pUC9, pEMBL8 and pEMBL9. Gene 41:337-342.
22. Tucker, W. T., C. A. Miller, and S. N. Cohen. 1984. Structural and functional analysis of the par region of the pSC101 plasmid. Cell 38:191-201.
23. Zhang, L., A. Al-Hendy, P. Toivanen, and M. Skurnik. 1993. Genetic organization and sequence of the rfb gene cluster of Yersinia enterocolitica serotype O:3: similarities to the dTDP-L-rhamnose biosynthesis pathway of Salmonella and to the bacterial polysaccharide transport systems. Mol. Microbiol. 9:309-321.