Isolation and characterization of rcsB mutations that affect colanic acid capsule synthesis in Escherichia coli K-12

Journal of Bacteriology

Volumn 179, Issue 13, 1997, Pages 4328-4335

  Gupte, Geeta ^a,b   Woodward, Cora ^b   Stout, Valerie ^b  

^a NATIONAL CANCER INSTITUTE   (United States)

^b ARIZONA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; POLYSACCHARIDE; REGULATOR PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BACTERIAL MEMBRANE; BACTERIUM MUTANT; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POINT MUTATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; STRAIN DIFFERENCE; STRUCTURE ACTIVITY RELATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA; ESCHERICHIA COLI; ESCHERICHIA COLI K12;

EID: 0030811369     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.13.4328-4335.1997     Document Type: Article

References (43)

1. Bourret, R. B., J. F. Hess, and M. I. Simon. 1990. Conserved aspartate residues and phosphorylation in signal transduclion by the chemotaxis protein CheY. Proc. Natl. Acad. Sci. USA 87:41-45.
2. Bourret, R. B., S. K. Drake, S. A. Chervitz, M. I. Simon, and J. J. Falke. 1993. Activation of the phosphosignaling protein CheY. Analysis of activated mutations by 19F NMR and protein engineering. J. Biol. Chem. 268:13089-13096.
3. Brill, J. A., C. Quinlan-Walshe, and S. Gottesman. 1988. Fine-structure mapping and identification of two regulators of capsule synthesis in Escherichia coli K-12. J. Bacteriol. 170:2599-2611.
4. Cheung, C. T., S. L. Niemela, and R. Miller. 1989. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. USA 86:2172-2175.
5. Costerton, J. W., and R. T. Irvin. 1981. The bacterial glycocalyx in nature and disease. Annu. Rev. Microbiol. 35:299-324.
6. Costerton, J. W., K.-J. Cheng, G. G. Geesey, T. I. Ladd, J. C. Nickel, M. Dasgupta, and T. J. Marrie. 1987. Bacterial biofilms in nature and disease. Annu. Rev. Microbiol. 41:435-464.
7. Enquist, L. W., and R. A. Weisberg. 1977. A genetic analysis of the att-int-xis region of coliphage lambda. J. Mol. Biol. 111:97-120.
8. Genetics Computer Group. 1991. Program manual for the University of Wisconsin package, version 7. University of Wisconsin, Madison.
9. Gervais, F. G., and G. R. Drapeau. 1992. Identification, cloning and characterization of rcsF, a new regulator gene for exopolysaccharide synthesis that suppresses the division mutation fisZ184 in Escherichia coli K-12. J. Bacteriol. 174:8016-8022.
10. Goebel, W. F. 1963. Colanic acid. Proc. Natl. Acad. Sci. USA 49:464-471.
11. Gottesman, S. 1996, Personal communication.
12. Gottesman, S. 1995. Regulation of capsule synthesis: modification of the two-component paradigm by an accessory unstable regulator, p. 253-262. In J. A. Hoch and T. J. Silhavy (ed.), Two-component signal transduction. ASM Press, Washington, D.C.
13. Gottesman, S., P. Trisler, and A. Torres-Cabassa. 1985. Regulation of capsular polysaccharide synthesis in Escherichia coli K-12: characterization of three regulatory genes. J. Bacteriol. 162:1111-1119.
14. Gottesman, S., and V. Stout. 1991. Regulation of capsular polysaccharide synthesis in Escherichia coli K-12. Mol. Microbiol. 5:1599-1606.
15. Hess, J. F., R. B. Bourret, and M. I. Simon. 1988. Histidine phosphorylation and phosphoryl group transfer in bacterial chemotaxis. Nature (London) 336:139-143.
16. Hess, J. F., K. Oosawa, P. Matsumura, and M. I. Simon. 1987. Protein phosphorylation is involved in bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 84:7609-7613.
17. Jann, K., and B. Jann. 1987. Polysaccharide antigens of Escherichia coli. Rev. Infect. Dis. 9:S517-S526.
18. Jin, S., R. K. Prusti, T. Roitsch, R. G. Ankenbauer, and E. W. Nester. 1990. Phosphorylation of the VirG protein of Agrobacterium tumefaciens by the autophosphorylated VirA protein: essential role in biological activity of VirG. J. Bacteriol. 172:4945-4950.
19. Keener, J., and S. Kustu. 1988. Protein kinase and phosphoprotein phosphatase activities of nitrogen regulatory proteins NTRB and NTRC of enteric bacteria: roles of the conserved amino-terminal domain of NTRC. Proc. Natl. Acad. Sci. USA 85:4976-4980.
20. Klose, K. E., D. S. Weiss, and S. Kustu. 1993. Glutamate at the site of phosphorylation of nitrogen-regulatory protein NTRC mimics aspartyl-phosphate and activates the protein. J. Mol. Biol. 232:67-78.
21. Lopez Torres, A. J. 1995. Regulation and role of colanic acid capsule in Escherichia coli K-12. Ph.D. dissertation. Arizona State University, Tempe.
22. Lukat, G. S., B. H. Lee, J. M. Mottonen, A. M. Stock, and J. B. Stock. 1991. Roles of the highly conserved aspartate and lysine residues in the response regulator of bacterial chemotaxis. J. Bacteriol. 266:8348-8354.
23. Lukat, G. S., A. M. Stock, and J. B. Stock. 1990. Divalent metal ion binding to the CheY protein and its significance to phosphotransfer in bacterial chemotaxis. Biochemistry 29:5436-5442.
24. Markovitz, A. 1977. Genetics and regulation of bacterial capsular polysaccharide biosynthesis and radiation sensitivity, p. 415-462. In I. W. Sutherland (ed.). Surface carbohydrates of the prokaryotic cell. Academic Press, New York, N.Y.
25. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory. Cold Spring Harbor, N.Y.
26. Mizusawa, S., and D. Ward. 1982. A bacteriophage lambda vector for cloning with BamH1 and Sau3A. Gene 20:317-322.
27. Ninfa, A. J., and B. Magasanik. 1986. Covalent modification of the glnG product, NRI, by the glnL product. NRII, regulates the transcription of the glnALG operon in Escherichia coli. Proc. Natl. Acad. Sci. USA 83:5909-5913.
28. Parkinson, J. S., and E. C. Kofoid. 1992. Communication modules in bacterial signaling proteins. Annu. Rev. Genet. 26:71-112.
29. Pazour, G. J., C. N. Ta, and A. Das. 1992. Constitutive mutations of Agrobacterium tumefaciens transcriptional activator VirG. J. Bacteriol. 174:4169-4173.
30. Roman, S. J., M. Meyers, K. Volz, and P. Matsumura. 1992. A chemotactic signaling surface on CheY defined by suppressors of flagellar switch mutations. J. Bacteriol. 174:6247-6255.
31. Sanders, D. A., B. L. Gillece-Castro, A. M. Stock, A. L. Burlingame, and D. E. Koshland, Jr. 1989. Identification of the site of phosphorylation of the chemotaxis response regulator protein, CheY. J. Biol. Chem. 264:21770-21778.
32. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463-5467.
33. Sasse-Dwight, S., and J. U. Gralla. 1988. Probing the Escherichia coli glnALG upstream activation mechanism in vivo. Proc. Natl. Acad. Sci. USA 85:8934-8940.
34. Silhavy, T. J., M. L. Berman, and L. W. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
35. Sockett, H., S. Yamaguchi, M. Kihara, V. M. Irikura, and R. M. Macnab. 1992. Molecular analysis of the flagellar switch protein film of Salmonella typhimurium. J. Bacteriol. 174:793-806.
36. Stevenson, G., K. Andrianopoulos, M. Hobbs, and P. R. Reeves. 1996. Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid. J. Bacteriol. 178:4885-4893.
37. Stout, V. 1996. Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Escherichia coli K-12. J. Bacteriol. 178: 4273-4280.
38. 37a.Stout, V. Unpublished data.
39. Stout, V., and S. Gottesman. 1990. RcsB and RcsC: a two-component regulator of capsule synthesis in Escherichia coli K-12. J. Bacteriol. 172:659-669.
40. Stout, V., A. Torres-Cabassa, M. R. Maurizi, D. Gutnick, and S. Gottesman. 1991. RcsA, an unstable positive regulator of capsular polysaccharide synthesis. J. Bacteriol. 173:1738-1747.
41. Torres-Cabassa, A., and S. Gottesman. 1987. Capsule synthesis in Escherichia coli K-12 is regulated by proteolysis. J. Bacteriol. 169:981-989.
42. Trisler, P., and S. Gottesman. 1984. Ion transcriptional regulation of genes necessary for capsular polysaccharide synthesis in Escherichia coli K-12. J. Bacteriol. 160:184-191.
43. Volz, K., and P. Matsumura. 1991. Crystal structure of Escherichia coli CheY refined at 1.7°A resolution. J. Biol. Chem. 266:15511-15519.