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2+-sensitive fluorescent dye fura 2-AM, as described (7). Inhibitors were applied 10 min before stimulation with glutamate. Ratio values varied among sets of experiments mainly because different objective lenses were used.
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3 was separated by Bio-Rad AG1X8 chromatography.
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2+ imaging; L. G. Sayers and A. Tanaka for critical reading of the manuscript; K. Haga, T. Haga, and K. Kimura for discussions; and Y. Kaziro for his encouragement. Supported by grants from the Ministry of Education, Science, and Culture of Japan (H.U. and T.Y.).
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