Identification of cytochrome P4503A as the major enzyme sub-family responsible for the metabolism of 22,23-dihydro-13-O-[(2-methoxyethoxy)-methyl]-avermectin B1 aglycone by rat liver microsomes

Xenobiotica

Volumn 27, Issue 10, 1997, Pages 985-994

  Zeng, Z ^a   Andrew, N W ^a   Halley, B A ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AVERMECTIN DERIVATIVE; CYTOCHROME P450 ISOENZYME; DEXAMETHASONE; DRUG METABOLITE; PHENOBARBITAL; TROLEANDOMYCIN;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DEMETHYLATION; DRUG METABOLISM; DRUG OXIDATION; ENZYME ANALYSIS; ENZYME INDUCTION; LIVER MICROSOME; NONHUMAN; RAT;

ANIMALS; ARYL HYDROCARBON HYDROXYLASES; CYTOCHROME P-450 CYP3A; CYTOCHROME P-450 ENZYME SYSTEM; ENZYME INHIBITORS; IVERMECTIN; MALE; MICROSOMES, LIVER; OXIDOREDUCTASES, N-DEMETHYLATING; PROADIFEN; RATS; RATS, SPRAGUE-DAWLEY;

EID: 0030808483     PISSN: 00498254     EISSN: None     Source Type: Journal    
DOI: 10.1080/004982597239967     Document Type: Article

References (14)

1. BURG, R. W. and STAPLEY, E. O., 1989, Isolation and characterization of the producing organism. In Ivermectin and Avermectin, edited by W. C. Campbell (New York: Springer), pp. 24-32.
2. COOPER, K. O., REIK, L. M., JAYYOSI, Z., BANDIERA, S., KELLEY, M., RYAN, D. E., DANIEL, R., MCCLUSKEY, S. A., LEVIN, W. and THOMAS, P. E., 1993, Regulation of two members of the steroid-inducible cytochrome P450 subfamily (3A) in rats. Archives of Biochemistry and Biophysics, 301, 345-354.
3. FISHER, M. H. and MROZIK, H., 1989, Chemistry. In Ivermectin and Avermectin, edited by W. C. Campbell (New York: Springer), pp. 1-23.
4. GEMZIK, B., GREENWAY, D., NEVINS, C. and PARKINSON, A., 1992, Regulation of two electrophoretically distinct proteins recognized by antibody against rat liver cytochrome-P450-3A1. Journal of Biochemical Toxicology, 7, 43-52.
5. HUSKEY, S.-E. W., Doss, G. A., MILLER, R. R., SCHOEN, W. R. and CHIU, S.-H. L., 1994, N-glucuronidation reactions, II. Relative N-glucuronidation reactivity of methylbiphenyl tetrazole, methylbiphenyl triazole, and methylbiphenyl imidazole in rat, monkey, and human hepatic microsomes. Drug Metabolism and Disposition, 22, 651-658.
6. MROZIK, H., LINN, B. O., ESKOLA, P., LUSI, A., MATZUK, A., PREISER, F. A., OSTLIND, D. A., SCHAEFFER, J. M. and FISHER, M. H., 1989, Syntheses and biological activities of 13-substituted avermectin aglycons. Journal of Medicinal Chemistry, 32, 376-381.
7. REIK, L. M., LEVIN, W., RYAN, D. E., MAINES, S. L. and THOMAS, P. E., 1985, Monoclonal antibodies distinguish among isozymes of cytochrome P-450b subfamily. Archives of Biochemistry and Biophysics, 242, 365-382.
8. SHOOP, W. L., MROZIK, H. and FISHER, M. H., 1995, Structure and activity of avermectins and milbemycin in animal health. Veterinary Parasitology, 59, 139-156.
9. SOUCEK, P. and GUT, I., 1992, Review. Cytochromes P-450 in rats: structures, functions, properties and relevant human forms. Xenobiotica, 22, 83-103.
10. THOMAS, P. E., BANDIERA, S., REIK, L. M., MAINES, S. L., RYAN, D. E. and LEVIN, W., 1987, Polyclonal and monoclonal antibodies as probes of rat hepatic cytochrome P-450 isozymes. Federation proceedings, 46, 2563-2566.
11. THOMAS, P. E., REIK, L. M., RYAN, D. E. and LEVIN, W., 1984, Characterization of nine monoclonal antibodies against rat hepatic cytochrome P-450c. Journal of Biological Chemistry, 259, 3890-3899.
12. WATKINS, P. B., WRIGHTON, S. A., MAUREL, P., SCHUETZ, E. G., MENDEZ-PICON, G., PARKER, G. A. and GUZELIAN, P. S., 1985, Identification of an inducible form of cytochrome P450 in human liver. Proceedings of National Academy of Sciences, USA, 82, 6310-6314.
13. WAXMAN, D. J., ATTISANO, C., GUENGERICH, F. P. and LAPENSON, D. P., 1988, Human liver microsomal steroid metabolism: identification of the major microsomal steroid hormone 6β-hydroxylase cytochrome P-450 enzyme. Archives of Biochemistry and Biophysics, 263, 424-436.
14. ZENG, Z., ANDREW, N. W., WODA, J. M., HALLEY, B. A., CROUCH, L. S. and WANG, R. W., 1996, Role of cytochrome P450 isoforms in the metabolism of abamectin and ivermectin in rats. Journal of Agricultural and Food Chemistry, 44, 3374-3378.