A synthetic heparin/heparan sulfate-like decasaccharide releases lipase activity in vivo. Chemical synthesis and biological activity.

Bioorganic and Medicinal Chemistry Letters

Volumn 7, Issue 15, 1997, Pages 2067-2070

  Petitou, M ^a   Duchaussoy, P ^a   Bernat, A ^a   Hoffmann, P ^a   Herbert, J M ^a  

^a SANOFI   (France)

Author keywords

[No Author keywords available]

Indexed keywords

HEPARIN FRAGMENT; TRIACYLGLYCEROL LIPASE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CARBOHYDRATE SYNTHESIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME BINDING; IN VITRO STUDY; INTRAVENOUS DRUG ADMINISTRATION; MALE; NONHUMAN; RAT;

EID: 0030808131     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(97)00361-2     Document Type: Article

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13. 1,2 3.5 Hz).
14. 14C]oleylglycerol with unlabeled trioleylglycerol (both in hexane) at the day of the experiment. Hexane was evaporated under a stream of nitrogen and the substrate was incorporated into 5% gum arabic by sonication. The incubation medium contained 20% of such substrate emulsion, 10% rat serum, 100 mM NaCl, 2% BSA and 40 mM Tris-HCl buffer pH 8.4. Incubation was carried out for 1 h at 28 °C. The hydrolyzed fatty acids were extracted by a mixture of methanol/chloroform/heptane 1.4/1.25/1 v/v/v and counted for radioactivity in a liquid scintillation counter. Enzyme activity was expressed as fatty acids μmol released/ml/h.