Calibration of the automated z-axis of a microscope using focus functions

Journal of Microscopy

Volumn 186, Issue 3, 1997, Pages 270-274

  Boddeke, F R ^a   Van Vliet, L J ^a   Young, I T ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

Author keywords

3D microscopy; Automated microscopy; Calibration

Indexed keywords

AUTOMATION; CCD CAMERAS; MICROSCOPES;

3-D MICROSCOPY; AUTOMATED MICROSCOPE; AUTOMATED MICROSCOPY; BAR PATTERN; FOCUS FUNCTION; MICROSCOPE SYSTEMS; OPTICAL AXIS; SIMPLE++; STEP SIZE; THREE-DIMENSIONAL MICROSCOPY;

CALIBRATION;

ARTICLE; AUTOMATION; MICROSCOPE; POSITION; PRIORITY JOURNAL; THREE DIMENSIONAL IMAGING;

EID: 0030807524     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1997.2060766.x     Document Type: Article

References (7)

1. Boddeke, F.R., van Vliet, L.J., Netten, H. & Young, I.T. (1994) Autofocusing in microscopy based on the OTF & sampling. BioImaging, 2, 193-203.
2. Hell, S., Reiner, G., Cremer, C. & Stelzer, E.H.K. (1993) Aberrations in confocal fluorescence microscopy induced by mismatches in refractive index. J. Microsc. 169, 391-405.
3. Jacobsen, H. & Hell, S.W. (1995) Effect of the specimen refractive index on the imaging of a confocal fluorescence microscope employing high aperture oil immersion lenses. BioImaging, 3, 39-47.
4. Mason, D.C. & Green, D.K. (1975) Automatic focusing of a computer-controlled microscope. IEEE Trans. Biomed. Eng. 22, 312-317.
5. Mullikin, J., van Vliet, L.J., Netten, H., Boddeke, F.R., van der Feltz, G.W. & Young, I.T. (1994) Methods for CCD camera characterization. SPIE, 2173, 73-84.
6. Visser, T.D., Oud, J.L. & Brakenhoff, G.J. (1992) Refractive index & axial distance measurements in 3-D microscopy. Optik, 90, 17-19.
7. Young, I.T., Zagers, R., van Vliet, L.J., Mullikin, J., Boddeke, F. & Netten, H. (1993) Depth-of-focus in microscopy. SCIA'93, Proc. 8th Scandinavian Conference on Image Analysis. pp. 493-498. NOBIM, Norway.