The heat-shock protein HslVU from Escherichia coli is a protein-activated ATPase as well as an ATP-dependent proteinase

European Journal of Biochemistry

Volumn 247, Issue 3, 1997, Pages 1143-1150

  Seol, Jae Hong ^a   Yoo, Soon Ji ^a   Shin, Dong Hun ^a   Shim, Yoon Kyung ^a   Kang, Man Sik ^a   Goldberg, Alfred L ^b   Chung, Chin Ha ^a  

^a Seoul National University   (South Korea)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Adenosinetriphosphate; ATP dependent protease; Heat shock protein; HsLU; HslV

Indexed keywords

ADENOSINE TRIPHOSPHATASE; HEAT SHOCK PROTEIN; PROTEINASE;

ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; IMMUNOPRECIPITATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION;

ESCHERICHIA COLI;

EID: 0030804951     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.01143.x     Document Type: Article

References (49)

1. Goldberg, A. L. (1992) The mechanism and function of ATP-dependent protease in bacterial and animal cells, Eur. J. Biochem. 203, 9-23.
2. Gottesman, S. & Maurizi, M. R. (1992) Regulation by proteolysis: Energy-dependent proteases and their targets, Microbiol. Rev. 56, 592-621.
3. Hilt, W. & Wolf, D. H. (1995) Proteasomes of the yeast S. cerevisiae: genes, structure and functions, Mol. Biol. Rep. 21, 3-10.
4. Tanaka, K. (1995) Molecular biology of proteasomes, Mol. Biol. Rep. 21, 21-26.
5. Seufert, W. & Jentsch, S. (1992) In vivo function of the proteasome in the ubiquitin pathway, EMBO J. 11, 3077-3080.
6. Palombella, V. J., Rando, O. J., Goldberg, A. L. & Maniatis, T. (1994) The ubiquitin-proteasome pathway is required for processing the NF-κB1 precursor protein and the activator of NF-κB, Cell 78, 773-785.
7. Ciechanover, A. (1994) The ubiquitin-proteasome proteolytic pathway, Cell 79, 13-21.
8. Palmen, A., Mason, G. F., Paramio, J. M., Knecht, E. & Rivett, A. J. (1994) Changes in proteasome localization during the cell cycle, Eur. J. Cell. Biol. 64, 163-175.
9. Rechsteiner, M., Hoffman, L. & Dubiel, W. (1993) The multicatalytic and 26S proteases, J. Biol. Chem. 268, 6065-6068.
10. Tanahashi, N., Tsurumi, C., Tamura, T. & Tanaka, K. (1993) Molecular structures of 20S and 26S proteasomes, Enzyme Protein 47, 241-251.
11. Peters, J. M., Franke, W. W. & Kleinschmidt, J. A. (1994) Distinct 19S and 20S subcomplexes of the 26S proteasome and their distribution in the nucleus and the cytoplasm, J. Biol. Chem. 269, 7709-7718.
12. Armon, T., Ganoth, D. & Hershko, A. (1990) Assembly of the 26S complex that degrades proteins ligated to ubiquitin is accompanied by the formation of ATPase activity, J. Biol. Chem. 265, 20723-20726.
13. Chung, C. H. (1993) Proteases in Escherichia coli, Science 262, 372-374.
14. Maurizi, M. R. (1992) Proteases and protein degradation in Escherichia coli, Experientia 48, 178-201.
15. Katayama-Fujimura, Y., Gottesman, S. & Maurizi, M. R. (1987) A multiple component, ATP-dependent protease from Escherichia coli, J. Biol. Chem. 262, 4477-4485.
16. Hwang, B. J., Woo, K. M., Goldberg, A. L. & Chung, C. H. (1988) Protease Ti, a new ATP-dependent protease in Escherichia coli contains protein-activated ATPase and proteolytic functions in distinct subunits, J. Biol. Chem. 263, 8727-8734.
17. Tomoyasu, T., Gamer, J., Bukau, B., Kanemori, M., Mori, H., Rutman, A. J., Oppenheim, A. B., Yura, T. Yamanaka, K., Niki, H., Hiraga, S. & Ogura, T. (1995) Escherichia coli FtsH is a membrane-bound, ATP-dependent protease which degrades the heatshock transcription factor σ 32, EMBO J. 14, 2551-2560.
18. Waxman, L. & Goldberg, A. L. (1982) Protease La from Escherichia coli hydrolyzed ATP and proteins in a linked fashion, Proc. Natl Acad. Sci. USA 79, 4883-4887.
19. Menon, A. S. & Goldberg, A. L. (1987) Binding of nucleotides to the ATP-dependent protease La from Escherichia coli, J. Biol. Chem. 262, 14921-14928.
20. Gottesman, S., Squires, C., Pichersky, E., Carrington, M., Hobbs, M., Mattick, J. S., Dalryple, B., Kuramitsu, H., Shioza, T. Foster, T., Clark, W. C., Ross, B., Squires, C. L. & Maurizi, M. R. (1990) Conservation of regulatory subunit for the Clp ATP-dependent protease in prokaryotes and eukaryotes, Proc. Natl Acad. Sci. USA 87, 3513-3517.
21. Walker, J. E., Saraste, M., Runswick, M. J. & Gay, J. N. (1982) Distantly related sequences in the α- and β-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold, EMBO J. 1, 945-951.
22. Gottesman, S., Clark, W. P., de Crecy-Lagard, V. & Maurizi, M. R. (1993) ClpX, an alternative subunit for the ATP-dependent Clp protease in Escherichia coli, J. Biol. Chem. 268, 22618-22626.
23. Wojtkowiak, D., Georgopoulos, C. & Zylicz, M. (1993) Isolation and characterization of ClpX, a new ATP-dependent specificity component of the Clp protease of Escherichia coli, J. Biol. Chem. 268, 22609-22617.
24. Mhammedi-Alaoui, A., Pato, M., Gama, M. J. & Toussaint, A. (1994) A new component of bacteriophage Mu replicative transposition machinery: the Escherichia coli ClpX protein, Mol. Microbiol. 11, 1109-1116.
25. Lehnherr, H. & Yarmolinsky, M. B. (1995) Addiction protein Phd of plasmid prophage P1 is a substrate of the ClpXP serine protease of Escherichia coli, Proc. Natl Acad. Sci. USA 92, 3274-3277.
26. Chuang, S. E., Burland, V., Plunket, G. III, Daniels, D. L. & Blattner, F. R. (1993) Sequence analysis of four new heat-shock genes constituting the hslTS/ibpAB and hslVU operons in Escherichia coli, Gene 134, 1-6.
27. Rohrwild, M., Coux, O., Huang, H. C., Moerschell, R. P., Yoo, S. J., Seol, J. H., Chung, C. H. & Goldberg, A. L. (1996) HslV-HslU: A novel ATP-dependent protease complex in Escherichia coli related to the eukaryotic proteasome, Proc. Natl Acad. Sci. USA 93, 5808-5813.
28. Yoo, S. J., Seol, J. H., Shin, D. H., Rohrwild, M., Rang, M. S., Tanaka, K., Goldberg, A. L. & Chung, C. H. (1996) Purification and characterization of the heat shock proteins HslV ad HslU that form a new ATP-dependent protease in Escherichia coli, J. Biol. Chem. 271, 14035-14040.
29. Missiakas, D., Schwager, F., Betton, J.-M., Georgopoulos, C. & Raina, S. (1997) Identification and characterization of HslV HslU (ClpQ ClpY) proteins involved in overall proteolysis of misfolded proteins in Escherichia coli, EMBO J. 15, 6899-6909.
30. Seemüller, E., Lupas, A., Stock, D., Löwe, J., Huber, R. & Baumeister, W. (1995) Proteasome from Thermoplasma acidophilum: a threonine protease, Science 268, 579-582.
31. Fenteany, G., Stanaert, R. F., Lane, W. S., Choi, S., Corey, E. J. & Schreiber, S. L. (1995) Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin, Science 268, 726-731.
32. Markwell, M. A. K. (1982) Protein determination in membrane and lipoprotein samples: manual and automated procedures, Anal. Biochem. 125, 427-432.
33. Hirs, C. H. W. (1967) Performic acid oxidation, Methods Enzymol. 11, 197-199.
34. Woo, K. M., Chung, W. J., Ha, D. B., Goldberg, A. L. & Chung, C. H. (1989) Protease Ti from Escherichia coli requires ATP hydrolysis for protein breakdown but not for hydrolysis of small peptides, J. Biol. Chem. 264, 2088-2091.
35. Kirn, K. I., Back, S. H., Hong, Y. M., Kang, M. S., Ha, D. B., Goldberg, A. L. & Chung, C. H. (1995) Purification and characterization of protease Ci, a cytoplasmic metalloendoprotease in Escherichia coli, J. Biol. Chem. 270, 29799-29805.
36. Ames, B. (1966) Assays of inorganic phosphate, total phosphate and phosphatase, Methods Enzymol, 8, 115-118.
37. Bradford, M. M. (1976) A rapid and sensitive method of quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72, 248-254.
38. Hwang, B. J., Park, W. J., Chung, C. H. & Goldberg, A. L. (1987) Escherichia coli contains a soluble ATP-dependent protease (Ti) distinct from protease La, Proc. Natl Acad. Sci. USA 84, 5550-5554.
39. Seol, J. H., Yoo, S. J., Kim, K. I., Kang, M. S., Ha, D. B. & Chung, C. H. (1994) The 65-kDa protein derived from the internal translational site of the clpA gene inhibits the ATP-dependent protease Ti in Escherichia coli, J. Biol. Chem. 269, 29468-29473.
40. Woo, K. M., Kim, K. I., Goldberg, A. L., Ha, D. B. & Chung, C. H. (1992) The heat-shock protein ClpB in Escherichia coli is a protein-activated ATPase, J. Biol. Chem. 267, 20429-20434.
41. Kessel, M., Wu, W., Gottesman, S., Kocsis, E., Stevens, A. C. & Maurizi, M. R. (1996) Six-fold rotational symmetry of ClpQ, the E. coli homolog of the 20S proteasome, and its ATP-dependent activator, ClpY, FEBS Lett. 398, 274-278.
42. Rohrwild, M., Pfeifer, G., Santarius, U., Müller, S. A., Huang, H.-C., Engel, A., Baumeister, W. & Goldberg, A. L. (1997) The ATP-dependent HslVU protease from Escherichia coli is a four-ring structure resembling the proteasome, Nature Struct. Biol. 4, 133-139.
43. Levchenko, I., Luo, L. & Baker, T. A. (1995) Dissembly of the Mu transposase tetramer by the ClpX chaperone, Genes & Dev. 9, 2399-2408.
44. Wawrzynow, A., Wojtkowiak, D., Marszalek, J., Banecki, B., Jonsen, M., Graves, B., Georgopoulos, C. & Zylicz, M. (1995) The ClpX heat-shock protein of Escherichia coli, the ATP-dependent substrate specificity component of the ClpP-ClpX protease, is a novel molecular chaperone, EMBO J. 14, 1867-1877.
45. Wickner, S., Gottesman, S., Skowyra, D., Hoskins, J., McKenney, K. & Maurizi, M. R. (1994) A molecular chaperone, ClpA, functions like DnaK and DnaJ, Proc. Natl Acad. Sci. USA 91, 12218-12222.
46. Waxman, L. & Goldberg, A. L. (1986) Selectivity of intracellular proteolysis: protein substrates activate the ATP-dependent protease (La), Science 232, 500-503.
47. Akopian, T. N., Kisselev, A. F. & Goldberg, A. L. (1997) Processive degradation of proteins and other catalytic properties of the proteasome from Thermoplasma acidophilum, J. Biol. Chem. 272, 1791-1798.
48. Goldberg, A. L., Moerschell, R. P., Chung, C. H. & Maurizi, M. R. (1994) ATP-dependent protease La (Lon) from Escherichia coli, Methods Enzymol. 244, 350-375.
49. Maurizi, M. R., Thompson, M. W., Singh, S. K. & Kim, S. H. (1994) Endopeptidase Clp: ATP-dependent Clp protease from Escherichia coli, Methods Enzymol. 244, 314-331.