The yeast Prp3 protein is a U4/U6 snRNP protein necessary for integrity of the U4/U6 snRNP and the U4/U6.U5 tri-snRNP

RNA

Volumn 3, Issue 10, 1997, Pages 1143-1152

  Anthony, James G ^a,b   Weidenhammer, Elaine M ^a,c   Woolford Jr , John L ^a  

^a CARNEGIE MELLON UNIVERSITY   (United States)

^b Digene Corporation   (United States)

^c UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

Pre mRNA splicing; Prp3p; S. cerevisiae; Spliceosome assembly; U4 U6 snRNP protein

Indexed keywords

ARTICLE; FUNGUS MUTATION; HEAT STRESS; IMMUNOPRECIPITATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; RNA SPLICING; SEQUENCE HOMOLOGY; SPLICEOSOME; STRAIN DIFFERENCE;

BLOTTING, NORTHERN; CENTRIFUGATION, DENSITY GRADIENT; DNA PROBES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FUNGAL PROTEINS; MUTATION; NUCLEAR PROTEINS; NUCLEIC ACID CONFORMATION; PRECIPITIN TESTS; RIBONUCLEOPROTEIN, U4-U6 SMALL NUCLEAR; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; RNA, MESSENGER; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SODIUM CHLORIDE; SPLICEOSOMES;

FUNGI; METAZOA;

EID: 0030801280     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (57)

1. Abovich N, Legrain P, Rosbash M. 1990. The yeast PRP6 gene encodes a U4/U6 small nuclear ribonucleoprotein particle (snRNP) protein, and the PRP9 gene encodes a protein required for U2 snRNP binding. Mol Cell Biol 10:6417-6425.
2. Arenas JE, Abelson JN. 1993. The Saccharomyces cerevisiae PRP21 gene product is an integral component of the prespliceosme. Proc Natl Acad Sci USA 90:6771-6775.
3. Ares M Jr, Weiser B. 1995. Rearrangement of snRNA structure during assembly and function of the spliceosome. Prog Nucleic Acid Res Mol Biol 50:131-159.
4. Ayadi L, Miller M, Banroques J. 1997. Mutations within the yeast U4/L6 snRNP protein Prp4 affect a late stage of spliceosome assembly. RNA 3:197-209.
5. Bantroques J, Abelson JN. 1989. PRP4: A protein of the yeast U4/U6 small nuclear ribonucleoprotein particle. Mol Cell Biol 9:3710-3719.
6. Behrens SE, Lührmann R. 1991. Immunoaffinity purification of a [U4/U6.U5] tri-snRNP from human cells. Genes & Dev 5:1439-1452.
7. Blanton S, Srinivasan A, Rymond BC. 1992. PRP38 encodes a yeast protein required for pre-mRNA splicing and maintenance of stable U6 small nuclear RNA levels. Mol Cell Biol 12:3939-3947.
8. Bordonné R, Banroques J. Abelson J, Guthrie C. 1990. Domains of yeast U4 spliceosomal RNA required for PRP4 protein binding, snRNP-snRNP interactions, and pre-mRNA splicing in vivo. Genes & Dev 4:1185-1196.
9. Brow DA, Guthrie C. 1988. Spliceosomal RNA U6 snRNA is remarkably conserved from yeast to mammals. Nature 334:213-218.
10. Brown JD, Beggs JD. 1992. Roles of PRP8 protein the the assembly of splicing complexes. EMBO J 11:3721-3729.
11. Cheng SC, Abelson J. 1987. Spliceosome assembly in yeast. Genes & Dev 1:1014-1027.
12. Dale RMK, McClure BA, Houchino JP. 1985. A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: Application to sequencing the corn mitochondrial 18S mDNA. Plasmid 13:31-40.
13. Dalrymple MA, Petersen-Bjorn S, Friesen JD, Beggs JD. 1989. The product of the PRP4 gene of S. cerevisiae shows homology to β subunits of G proteins. Cell 58:811-812.
14. Dieckman CL, Tzagoloff A. 1985. Assembly of the mitochondrial membrane system. J Biol Chem 260:1513-1530.
15. Fortner DM, Troy RG, Brow DA. 1994. A stem/loop in U6 RNA defines a conformational switch required for pre-mRNA splicing. Genes & Dev 8:221-233.
16. Galisson F, Legrain P. 1993 The biochemical defects of prp4-1 and prp6-1 yeast splicing mutants reveal that the PRP6 protein is required for the accumulation of the [U4/U6.U5] tri-snRNP. Nucleic Acids Res 21:1555-1562
17. Gozani O, Patton JG, Reed R. 1994. A novel set of spliceosomeassociated proteins and the essential splicing factor PSF bind stably to pre-mRNA prior to catalytic step II of the splicing reaction. EMBO J 13:3356-3367.
18. Guthrie C. 1996. The spliceosome is a dynamic ribonudeoprotein machine. The Harvey Lecture Series 90:59-80.
19. Hartwell LH. 1967. Macromolecular synthesis in temperature-sensitive mutants of yeast. J Bacteriol 93:1662-1670.
20. Hartwell LH, McLaughlin CS, Warner JR. 1970. Identification of ten genes that control ribosome formation in yeast. Mol Gen Genetics 109:42-56.
21. Hodges PE, Plumpton M, Beggs JD. 1993. Splicing factors in the yeast Saccharomyces cerevisiae. In: Krainer A, ed. Eukaryotic mRNA processing: Frontiers in molecular biology. Oxford: Oxford University Press.
22. Horowitz DS, Krainer AR. 1997. A human protein required for the second step of pre-mRNA splicing is functionally related to a yeast splicing factor. Genes & Dev 11:139-151.
23. Hu J, Xu Y, Schappert K, Harrington T, Wang A, Braga R, Mogridge J, Friesen JD. 1994. Mutational analysis of the PRP4 protein of Saccharomyces ceremsiae suggests domain structure and snRNP interactions. Nucleic Acids Res 22:1724-1734.
24. Kim SH, Lin RJ. 1996. Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing. Mol Cell Biol 16:6810-6819.
25. Krämer AR. 1996. The structure and function of proteins involved in mammalian pre-mRNA splicing. Annu Rev Biochem 65:367-409.
26. Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
27. Lamond AI, Konarska MM, Grabowski PJ, Sharp PA. 1988. Spliceosome assembly involves the binding and the release of U4 small nuclear ribonucleoprotein. Proc Natl Acad Sci USA 85:411-415.
28. Larkin JC, Woolford JL Jr. 1983. Molecular cloning and analysis of the CRV1 gene: A yeast ribosomal protein gene. Nucleic Acids Res 11:403-426.
29. Last RL, Maddock JR, Woolford JL Jr. 1987. Evidence for related functions of the RNA genes of Saccharomyces cerevisiae. Genetics 117:619-631.
30. Last RL, Stavenhagen JB, Woolford JL Jr. 1984. Isolation and characterization of the RNA2, RNA3 and RNA11 genes of Saccharomyces cerevisiae. Mol Cell Biol 4:2396-2405.
31. Last RL, Woolford JL Jr. 1986. Identification and nuclear localization of yeast pre-mRNA processing components: RNA2 and RNA3 proteins. J Cell Biol 103:2103-2112.
32. Lauber J, Plessel G, Prehn S, Will CL, Fabrizio P, Gröning K, Lane WS, Lührmann R. 1997. The human U4/U6 snRNP contains 60 and 90kD proteins that are structurally homologous to the yeast splicing factors Prp4p and Prp3p. RNA 3:926-941.
33. Legrain P, Chanfreau G. 1994. Pre-mRNA splicing: From intron recognition to catalysis. Bull Inst Pasteur 92:153-179.
34. Lin RJ, Newman AJ, Cheng SC, Abelson J. 1985. Yeast mRNA splicing in vitro. J Biol Chem 260:14780-14792.
35. Lossky M, Anderson GJ, Jackson SP, Beggs JD. 1987. Identification of yeast snRNP protein and the detection of snRNP-snRNP interactions. Cell 51:1019-1026.
36. Lührmann R, Kastner B, Bach M. 1990. Structure of spliceosomal snRNPs and their role in pre-mRNA splicing. Biochim Biophys Acta 1087:265-292.
37. Lustig AJ, Lin RJ, Abelson J. 1986. The yeast RNA gene products are essential for mRNA splicing in vitro. Cell 47:953-963.
38. Maddock JR, Roy J, Woolford JL Jr. 1996. Six novel genes necessary for pre-mRNA splicing in Saccharomyces cerevisiae. Nucleic Acids Res 24:1037-1044.
39. Maddock JR, Weidenhammer EM, Adams C, Lunz RL, Woolford JL Jr. 1994. Extragenic suppressors of Soccharomyces cerevisiac prp4 mutations identify a negative regulator of PRP genes. Genetics 136:833-547.
40. Moehle CM, Dixon CK, Jones EW. 1989. Processing pathway for protease B of Soccharomyces cerevisiae. J Cell Biol 108:309-325.
41. Moore MJ, Query CC, Sharp PA. 1993. Splicing of precursors to messenger RNAs by the spliceosome. In: Gesteland RF, Gesteland RF, Atkins, JF, eds. The RNA world. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press, pp 303-358.
42. Neubauer G, Gottschalk A, Fabrizio P, Séraphin B, Lührmann R, Mann M. 1997. Identification of the proteins of the yeast U1 small nuclear ribonucleoprotein complex by mass spectrometry. Proc Natl Acad Sci USA 94:385-390.
43. Newman A. 1994. Small nuclear RNAs and pre-mRNA splicing. Curr Opin Cell Biol 6:360-367.
44. Nilsen TW. 1994. RNA-RNA interactions in the spliceosome: Unraveling the ties that bind. Cell 78:1-4.
45. Norrander J, Kempe T, Messing J. 1983. Construction of improved M13 using oligonucleotide-directed mutagenesis. Gene 26:101-106.
46. Peterson-Björn S, Soltyk A, Beggs JD, Friesen JD. 1989. PRP4 (RNA4) from Saccharomyces cerevisiae: Its gene product is associated with the U4/U6 small nuclear ribonucleoprotein particle. Mol Cell Biol 9:3698-3709.
47. Rinke J, Appel B, Digweed M, Lührmann RL. 1985. Localization of a base-paired interaction between small nuclear RNAs U4 and C6 in intact U4/U6 ribonucleoprotein particles by psoralen crosslinking. J Mol Biol 185:721-731.
48. Rosbash M, Harris PKW, Woolford JL Jr, Teem JL. 1981. The effect of temperature-sensitive RNA mutants on the transcription products from cloned ribosomal protein genes of yeast. Cell 24:679-686.
49. Roy J, Kim K, Maddock JR, Anthony JG, Woolford JL Jr. 1995a. The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing. RNA 1:375-390.
50. Ruby SW, Abelson J. 1991. Pre-mRNA splicing in yeast. Trends Genet 7:79-85.
51. Ruby SW, Chang TS, Abelson J. 1993. Four yeast spliceosomal proteins (PRP5, PRP9, PRP11, and PRP21) interact to promote U2 snRNP binding to pre-mRNA. Gene & Dev 7:1909-1925.
52. Rymond BC, Rosbash M. 1992. Yeast pre-mRNA splicing. In: Jones EW, Pringle JR, Broach JR, eds. The molecular and cellular biology of the yeast Soccharomyces: Gene expression. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press. pp 143-192.
53. Sanger F, Nicklen S, Coulson AR. 1977. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 83:735-739.
54. Shannon KW, Guthrie C. 1991. Suppressors of a U4 snRNA mutation define a novel U6 snRNP protein with RNA-binding motifs. Genes & Dev 5:773-785.
55. Siliciano PG, Brow DA, Roiha H, Guthrie C. 1987. An essential snRNA from S. cerevisiae has properties predicted for U4 including interaction with a U6 like snRNA. Cell 50:585-592.
56. Weidenhammer EM, Ruiz-Noriega M, Woolford JL, Jr. 1997. Prp31p promotes the association of U4/U6.U5 tri-snRNP with prespliceosomes to form spliceosomes in Saccharomyces cerevisiae. Mol Cell Biol 17:3580-3588.
57. Xu Y, Peterson-Björn S, Friesen JD. 1990. The PRP4 (RNA4) protein of Soccharomyces cerevisiae is associated with the 5′ portion of the U4 small nuclear RNA. Mol Cell Biol 10:1217-1225.