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Volumn 119, Issue 39, 1997, Pages 9096-9102

Protein engineering using molecular assembly: Functional conversion of cytochrome C via noncovalent interactions

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHROME C;

EID: 0030800574     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9711775     Document Type: Article
Times cited : (64)

References (61)
  • 21
    • 0342752276 scopus 로고    scopus 로고
    • Scott, R. A., Mauk, A. G., Eds.; University Science Book: Sausalito, CA
    • (f) Hildebrandt, P. In Cytochrome c-A multidisciplinary approach; Scott, R. A., Mauk, A. G., Eds.; University Science Book: Sausalito, CA, 1996; p 285.
    • (1996) Cytochrome c-A Multidisciplinary Approach , pp. 285
    • Hildebrandt, P.1
  • 27
    • 1842325166 scopus 로고    scopus 로고
    • note
    • In the mixed lipid membrane of the lower content of 1a (less than 10%), it is reasonable to consider that 1a is homogeneously dispersed in the DMPC lipid. With increase of the 1a ratio, the lipid 1a easily aggregates to form a cluster in the mixed membrane. The sigmoidal curve for the binding affinity study suggests that such a cluster structure of 1a is needed for the Cyt-c binding. The N-demethylase activity was also found to be sigmoidal in the presence of the mixed lipid bilayer membrane.
  • 31
    • 70449234614 scopus 로고
    • The molecular extinction coefficient of native Cyt-c (reduced form) was reported to be 129.1/mM cm. Margoliash, E.; Frohwirt, N. J. Biochem. 1959, 71, 570.
    • (1959) J. Biochem. , vol.71 , pp. 570
    • Margoliash, E.1    Frohwirt, N.2
  • 34
    • 1842333386 scopus 로고    scopus 로고
    • note
    • For 1d-bound Cyt-c, the low spin state became predominant at 20 K, instead of the spin-state equilibrium at room temperature.
  • 37
    • 1842402386 scopus 로고    scopus 로고
    • note
    • Unfortunately, a strong CD band due to the chiral amide residue of the lipids 1a and 1d prevented us from estimating the α-helix region by CD spectroscopy.
  • 39
    • 12044249571 scopus 로고
    • ⊥ components are in the heme plane. The g signal is intensified when the direction of g tensor is parallel to the direction of the applied magnetic field. The simulation was conducted according to the same manner reported by us. (a) Hamachi, I.; Noda, S.; Kunitake, T. J. Am. Chem. Soc. 1991, 113, 9625.
    • (1991) J. Am. Chem. Soc. , vol.113 , pp. 9625
    • Hamachi, I.1    Noda, S.2    Kunitake, T.3
  • 44
    • 1842363991 scopus 로고    scopus 로고
    • note
    • Since the phosphate lipid 1d did not form a stable cast film, we could not evaluate the orientation of Cyt-c against the membrane comprising of 1d.
  • 47
    • 1842407319 scopus 로고    scopus 로고
    • note
    • 2 was consumed by 1a-bound Cyt-c in the absence of NNDA.
  • 51
    • 1842325675 scopus 로고    scopus 로고
    • note
    • The order of the magnitude of the heme-degradation rate was in good correspondence with that of the net N-demethylase activity.
  • 54
    • 1842401326 scopus 로고    scopus 로고
    • note
    • The phase-transition temperature of the lipid bilayer membranes was determined by differential scanning calorimeter (DSC). Although Figure 9 displayed the plot of the reaction rate at 38°C, similar dependence was obtained at the reaction temperature of 30°C.
  • 60
    • 1842402897 scopus 로고    scopus 로고
    • note
    • The morphology of all of the lipid bilayer membranes was a mixture of tube, disk, and stringlike structures.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.