The α-gliadin gene family. I. Characterization of ten new wheat α-gliadin genomic clones, evidence for limited sequence conservation of flanking dna, and Southern analysis of the gene family

Theoretical and Applied Genetics

Volumn 95, Issue 1-2, 1997, Pages 50-58

  Anderson, O D ^a   Litts, J C ^a,b   Greene, F C ^a,c  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

^b ONYX PHARMACEUTICALS   (United States)

^c AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

Gliadins; Gene family; Prolamines; Sequence conservation; Southern analysis; Storage proteins; Wheat

Indexed keywords

EID: 0030799308     PISSN: 00405752     EISSN: None     Source Type: Journal    
DOI: 10.1007/s001220050531     Document Type: Article

References (25)

1. Anderson OD (1991) Characterization of members of a pseudogene subfamily of the wheat α-gliadin storage protein genes. Plant Mol Biol 16:335-337
2. Anderson OD, Greene (1997) The α-gliadin gene family. II. Microsatellites within the coding sequence are responsible for protein size variation and contribute to pseudogene generation. Theor Appl Genet 95:59-65
3. Anderson OD, Litts JC, Gautier M-F, and Greene FC (1994) Nucleic acid sequence and chromosome assignment of a wheat storage-protein gene. Nucleic Acids Res 12:8129-8144
4. Anderson OD, Halford NG, Forde J, Yip R, Shewry PR, Greene FC (1988) Structure and analysis of the high-molecular-weight glutenin genes from Triticum aestirum cv Cheyenne. Proc 7th Int Wheat Genet Symp, pp 699-704. Institute of Plant Science Research (Pub.), Cambridge. Bable Press, Avon
5. Anderson OD, Greene FC, Litts JC (1991) Structure of the α-gliadin gene family from the bread wheat cultivar Cheyenne. In: Bushuk, Tkachuk (eds) Gluten proteins 1990. pp 640-645
6. D'Ovidio R, Lafiandra D, Tanzarella OA, Anderson OD, Greene FC (1991) Molecular characterization of bread wheat mutants lacking the entire cluster of chromosome 6A-controlled gliadin components. J Cereal Sci 14:125-129
7. D'Ovidio R, Tanzarella O, Masci S, Lafiandra D, Porceddu E (1992) RFLP and PCR analyses at Gli-1, Gli-2, Glu-1 and Glu-3 loci in cultivated and wild wheats. Hereditas 116:79-85
8. Dale RMK, McClure BA, Houchins JP (1985) A rapid single-stranded cloning strategy for producing a sequential series of overlapping clones for use in DNA sequencing: application to sequencing the corn mitochondrial 18srDNA. Plasmid 13:31-40
9. Garcia-Maroto F, Marana C, Garcio-Olmedo F, Carbonero P (1990) Nucleotide sequence of a cDNA encoding an α/β-type gliadin from hexaploid wheat (Triticum aestivum). Plant Mol Bio 14:867-868
10. Harberd NP, Bartels D, Thompson RD (1985) Analysis of the gliadin multigene loci in bread wheat using nullisomic-tetrasomic lines. Mol Gen Genet 198:234-242
11. Kasarda DD, Okita TW, Bernardin JE, Baecker PA, Nimmo CC, Lew EJ-L, Dietler MD, Greene FC (1984) Nucleic acid (cDNA) and amino-acid sequences of α-type gliadins from wheat (Triticum aestivum). Proc Natl Acad Sci USA 81:4712-4716
12. Lassner M, Dvorak J (1986) Preferential homogenization between adjacent and alternate subrepeats in wheat rDNA. Nucleic Acids Res 5499-5512
13. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
14. Okita TW, Cheesbrough V, Reeves CD (1985) Evolution and heterogeneity of the α-/β-type and γ-type gliadin DNA sequences. J Biol Chem 260:8203-8213
15. Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annu Rev Genet 38:141-153
16. Pomeranz Y (1988) Composition and functionality of wheat flour components. In: Pomeranz Y (ed) Wheat chemistry and technology, vol II. Am Assoc Cereal Chem Inc, St Paul, USA, pp 97-158
17. Rafalski JA, Scheets K, Metzler M, Peterson DM, Hedgcoth C, Söll D (1984) Developmentally regulated plant genes: the nucleotide sequence of a wheat gliadin genomic clone. EMBO J 3: 1409-1415
18. Reeves CD, Okita TW (1987) Analyses of α/β-gliadin genes from diploid and hexaploid wheats. Gene 52:257-266
19. Sabelli PA, Shewry PR (1991) Characterization and organization of gene families at the Gli-1 loci of bread and durum wheats by restriction-fragment analysis. Theor Appl Genet 83: 209-216
20. Sanchez-Pescador R, Urdea MS (1984) Use of unpurified primers in sequencing. DNA 3:339-343
21. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USa 74: 5463-5467
22. Shewry PR, Tatham AS, Kasarda DD (1992) Cereal proteins and coeliac disease. In: Marsh MN (ed) Coeliac disease. Blackwell Scientific Publications, London, pp 305-348
23. Shewry PR, Tatham As (1990) The prolamin storage proteins of cereal seeds: structure and evolution. Biochem J 267:1-12
24. Sumner-Smith M, Rafalski JA, Sugiyama T, Stoll M, Soll D (1985) Conservation and variability of wheat α/β-gliadin genes. Nucleic Acids Res 13:3905-3916
25. Weeks JT, Anderson OD, Blechl AE (1993) Rapid production of multiple independent lines of fertile transgenic wheat (Triticum aestivum). Plant Physiol 102:1077-1084