Efficient 5'-end labeling of oligonucleotides containing self- complementary sequences

BioTechniques

Volumn 23, Issue 4, 1997, Pages 658-660

  Quijada, L ^a   Moreira, D ^a   Soto, M ^a   Alonso, C ^a   Requena, J M ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DNA LIBRARY; DNA SEQUENCE; ENZYME ACTIVITY; GENE AMPLIFICATION; HYBRIDIZATION; LABELING INDEX; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; RNA SEQUENCE;

EID: 0030799077     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97234bm25     Document Type: Article

References (5)

1. m reduction: a method for analyzing the role of renaturation temperature in the polymerase chain reaction. Anal. Biochem. 209:284-290.
2. Deveraux, J., P. Haeberli and O. Smithies. 1984. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 12:387-395.
3. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. CSH Laboratory Press, Cold Spring Harbor, NY.
4. Varadaraj, K. and D.M. Skinner. 1994. Denaturants or cosolvents improve the specificity of PCR amplification of a G+C rich DNA using genetically engineered DNA polymerases. Gene 140:1-5.
5. Winship, P.R. 1989. An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide. Nucleic Acids Res. 17:1266.